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Qualitative hepatitis C virus RNA assay identifies active infection with sufficient viral load for treatment among Phetchabun residents in Thailand

The World Health Organization envisions the elimination of viral hepatitis by 2030 through reducing prevalence and transmission, increasing diagnostic screening, and expanding treatment coverage. Efforts to micro-eliminate hepatitis in Phetchabun province in Thailand, a region where the prevalence o...

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Autores principales: Pratedrat, Pornpitra, Nilyanimit, Pornjarim, Wasitthankasem, Rujipat, Posuwan, Nawarat, Auphimai, Chompoonut, Hansoongnern, Payuda, Pimsing, Napaporn, Ngamnimit, Saranya, Thongmai, Chaiwat, Phaengkha, Wijittra, Wanlapakorn, Nasamon, Vongpunsawad, Sompong, Poovorawan, Yong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9851543/
https://www.ncbi.nlm.nih.gov/pubmed/36656832
http://dx.doi.org/10.1371/journal.pone.0268728
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author Pratedrat, Pornpitra
Nilyanimit, Pornjarim
Wasitthankasem, Rujipat
Posuwan, Nawarat
Auphimai, Chompoonut
Hansoongnern, Payuda
Pimsing, Napaporn
Ngamnimit, Saranya
Thongmai, Chaiwat
Phaengkha, Wijittra
Wanlapakorn, Nasamon
Vongpunsawad, Sompong
Poovorawan, Yong
author_facet Pratedrat, Pornpitra
Nilyanimit, Pornjarim
Wasitthankasem, Rujipat
Posuwan, Nawarat
Auphimai, Chompoonut
Hansoongnern, Payuda
Pimsing, Napaporn
Ngamnimit, Saranya
Thongmai, Chaiwat
Phaengkha, Wijittra
Wanlapakorn, Nasamon
Vongpunsawad, Sompong
Poovorawan, Yong
author_sort Pratedrat, Pornpitra
collection PubMed
description The World Health Organization envisions the elimination of viral hepatitis by 2030 through reducing prevalence and transmission, increasing diagnostic screening, and expanding treatment coverage. Efforts to micro-eliminate hepatitis in Phetchabun province in Thailand, a region where the prevalence of hepatitis C virus (HCV) infection and liver cancer is higher than elsewhere in the country, began with evaluating the province-wide burden of HCV. Here, we describe a feasibility study to assess active HCV infection by screening Phetchabun residents ages 35 to 69 years for anti-HCV antibodies by using a rapid diagnostic test (RDT) at the point of care. Positive anti-HCV results were further evaluated for active infection using qualitative HCV RNA assay, followed by quantitative HCV viral load determination in a subset of samples. Currently, we have identified 6.2% (10,621/170,163) anti-HCV positive individuals, of whom 74.9% (3,930/5,246) demonstrated detectable viral RNA. Quantitative test found that 97.5% (1,001/1,027) had HCV viral load ≥5,000 IU/mL. Thus, primary screening with anti-HCV RDT followed by qualitative HCV RNA evaluation could identify active and chronic HCV infection in almost all individuals with a viral load ≥5,000 IU/mL, which is the current threshold for treatment dictated by Thailand’s National Health Security Office. Our data suggest that qualitative HCV RNA evaluation may obviate the need for the more expensive quantitative HCV viral load test and reduce a significant barrier toward HCV elimination in a middle-income country.
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spelling pubmed-98515432023-01-20 Qualitative hepatitis C virus RNA assay identifies active infection with sufficient viral load for treatment among Phetchabun residents in Thailand Pratedrat, Pornpitra Nilyanimit, Pornjarim Wasitthankasem, Rujipat Posuwan, Nawarat Auphimai, Chompoonut Hansoongnern, Payuda Pimsing, Napaporn Ngamnimit, Saranya Thongmai, Chaiwat Phaengkha, Wijittra Wanlapakorn, Nasamon Vongpunsawad, Sompong Poovorawan, Yong PLoS One Research Article The World Health Organization envisions the elimination of viral hepatitis by 2030 through reducing prevalence and transmission, increasing diagnostic screening, and expanding treatment coverage. Efforts to micro-eliminate hepatitis in Phetchabun province in Thailand, a region where the prevalence of hepatitis C virus (HCV) infection and liver cancer is higher than elsewhere in the country, began with evaluating the province-wide burden of HCV. Here, we describe a feasibility study to assess active HCV infection by screening Phetchabun residents ages 35 to 69 years for anti-HCV antibodies by using a rapid diagnostic test (RDT) at the point of care. Positive anti-HCV results were further evaluated for active infection using qualitative HCV RNA assay, followed by quantitative HCV viral load determination in a subset of samples. Currently, we have identified 6.2% (10,621/170,163) anti-HCV positive individuals, of whom 74.9% (3,930/5,246) demonstrated detectable viral RNA. Quantitative test found that 97.5% (1,001/1,027) had HCV viral load ≥5,000 IU/mL. Thus, primary screening with anti-HCV RDT followed by qualitative HCV RNA evaluation could identify active and chronic HCV infection in almost all individuals with a viral load ≥5,000 IU/mL, which is the current threshold for treatment dictated by Thailand’s National Health Security Office. Our data suggest that qualitative HCV RNA evaluation may obviate the need for the more expensive quantitative HCV viral load test and reduce a significant barrier toward HCV elimination in a middle-income country. Public Library of Science 2023-01-19 /pmc/articles/PMC9851543/ /pubmed/36656832 http://dx.doi.org/10.1371/journal.pone.0268728 Text en © 2023 Pratedrat et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Pratedrat, Pornpitra
Nilyanimit, Pornjarim
Wasitthankasem, Rujipat
Posuwan, Nawarat
Auphimai, Chompoonut
Hansoongnern, Payuda
Pimsing, Napaporn
Ngamnimit, Saranya
Thongmai, Chaiwat
Phaengkha, Wijittra
Wanlapakorn, Nasamon
Vongpunsawad, Sompong
Poovorawan, Yong
Qualitative hepatitis C virus RNA assay identifies active infection with sufficient viral load for treatment among Phetchabun residents in Thailand
title Qualitative hepatitis C virus RNA assay identifies active infection with sufficient viral load for treatment among Phetchabun residents in Thailand
title_full Qualitative hepatitis C virus RNA assay identifies active infection with sufficient viral load for treatment among Phetchabun residents in Thailand
title_fullStr Qualitative hepatitis C virus RNA assay identifies active infection with sufficient viral load for treatment among Phetchabun residents in Thailand
title_full_unstemmed Qualitative hepatitis C virus RNA assay identifies active infection with sufficient viral load for treatment among Phetchabun residents in Thailand
title_short Qualitative hepatitis C virus RNA assay identifies active infection with sufficient viral load for treatment among Phetchabun residents in Thailand
title_sort qualitative hepatitis c virus rna assay identifies active infection with sufficient viral load for treatment among phetchabun residents in thailand
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9851543/
https://www.ncbi.nlm.nih.gov/pubmed/36656832
http://dx.doi.org/10.1371/journal.pone.0268728
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