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Apiaceae FNS I originated from F3H through tandem gene duplication

BACKGROUND: Flavonoids are specialized metabolites with numerous biological functions in stress response and reproduction of plants. Flavones are one subgroup that is produced by the flavone synthase (FNS). Two distinct enzyme families evolved that can catalyze the biosynthesis of flavones. While th...

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Detalles Bibliográficos
Autores principales: Pucker, Boas, Iorizzo, Massimo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9851555/
https://www.ncbi.nlm.nih.gov/pubmed/36656808
http://dx.doi.org/10.1371/journal.pone.0280155
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author Pucker, Boas
Iorizzo, Massimo
author_facet Pucker, Boas
Iorizzo, Massimo
author_sort Pucker, Boas
collection PubMed
description BACKGROUND: Flavonoids are specialized metabolites with numerous biological functions in stress response and reproduction of plants. Flavones are one subgroup that is produced by the flavone synthase (FNS). Two distinct enzyme families evolved that can catalyze the biosynthesis of flavones. While the membrane-bound FNS II is widely distributed in seed plants, one lineage of soluble FNS I appeared to be unique to Apiaceae species. RESULTS: We show through phylogenetic and comparative genomic analyses that Apiaceae FNS I evolved through tandem gene duplication of flavanone 3-hydroxylase (F3H) followed by neofunctionalization. Currently available datasets suggest that this event happened within the Apiaceae in a common ancestor of Daucus carota and Apium graveolens. The results also support previous findings that FNS I in the Apiaceae evolved independent of FNS I in other plant species. CONCLUSION: We validated a long standing hypothesis about the evolution of Apiaceae FNS I and predicted the phylogenetic position of this event. Our results explain how an Apiaceae-specific FNS I lineage evolved and confirm independence from other FNS I lineages reported in non-Apiaceae species.
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spelling pubmed-98515552023-01-20 Apiaceae FNS I originated from F3H through tandem gene duplication Pucker, Boas Iorizzo, Massimo PLoS One Research Article BACKGROUND: Flavonoids are specialized metabolites with numerous biological functions in stress response and reproduction of plants. Flavones are one subgroup that is produced by the flavone synthase (FNS). Two distinct enzyme families evolved that can catalyze the biosynthesis of flavones. While the membrane-bound FNS II is widely distributed in seed plants, one lineage of soluble FNS I appeared to be unique to Apiaceae species. RESULTS: We show through phylogenetic and comparative genomic analyses that Apiaceae FNS I evolved through tandem gene duplication of flavanone 3-hydroxylase (F3H) followed by neofunctionalization. Currently available datasets suggest that this event happened within the Apiaceae in a common ancestor of Daucus carota and Apium graveolens. The results also support previous findings that FNS I in the Apiaceae evolved independent of FNS I in other plant species. CONCLUSION: We validated a long standing hypothesis about the evolution of Apiaceae FNS I and predicted the phylogenetic position of this event. Our results explain how an Apiaceae-specific FNS I lineage evolved and confirm independence from other FNS I lineages reported in non-Apiaceae species. Public Library of Science 2023-01-19 /pmc/articles/PMC9851555/ /pubmed/36656808 http://dx.doi.org/10.1371/journal.pone.0280155 Text en © 2023 Pucker, Iorizzo https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Pucker, Boas
Iorizzo, Massimo
Apiaceae FNS I originated from F3H through tandem gene duplication
title Apiaceae FNS I originated from F3H through tandem gene duplication
title_full Apiaceae FNS I originated from F3H through tandem gene duplication
title_fullStr Apiaceae FNS I originated from F3H through tandem gene duplication
title_full_unstemmed Apiaceae FNS I originated from F3H through tandem gene duplication
title_short Apiaceae FNS I originated from F3H through tandem gene duplication
title_sort apiaceae fns i originated from f3h through tandem gene duplication
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9851555/
https://www.ncbi.nlm.nih.gov/pubmed/36656808
http://dx.doi.org/10.1371/journal.pone.0280155
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