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Hypergravity enhances RBM4 expression in human bone marrow-derived mesenchymal stem cells and accelerates their differentiation into neurons

INTRODUCTION: The regulation of stem cell differentiation is important in determining the quality of transplanted cells in regenerative medicine. Physical stimuli are involved in regulating stem cell differentiation, and in particular, research on the regulation of differentiation using gravity is a...

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Autores principales: Teranishi, Masataka, Kurose, Tomoyuki, Nakagawa, Kei, Kawahara, Yumi, Yuge, Louis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Japanese Society for Regenerative Medicine 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9851867/
https://www.ncbi.nlm.nih.gov/pubmed/36712961
http://dx.doi.org/10.1016/j.reth.2022.12.010
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author Teranishi, Masataka
Kurose, Tomoyuki
Nakagawa, Kei
Kawahara, Yumi
Yuge, Louis
author_facet Teranishi, Masataka
Kurose, Tomoyuki
Nakagawa, Kei
Kawahara, Yumi
Yuge, Louis
author_sort Teranishi, Masataka
collection PubMed
description INTRODUCTION: The regulation of stem cell differentiation is important in determining the quality of transplanted cells in regenerative medicine. Physical stimuli are involved in regulating stem cell differentiation, and in particular, research on the regulation of differentiation using gravity is an attractive choice. We have shown that microgravity is useful for maintaining undifferentiated mesenchymal stem cells (MSCs). However, the effects of hypergravity on the differentiation of MSCs, especially on neural differentiation related to neural regeneration, have not been elucidated. METHODS: We induced neural differentiation of human bone marrow-derived MSCs (hbMSCs) for 10 days under normal gravity (1G) or hypergravity (3G) conditions using a gravity controller, Gravite®. HbMSCs were collected, and cell number and viability were measured 3 and 10 days after induction. RNA was also extracted from the collected hbMSCs, and the expression of neuron-associated genes and regulator markers of neural differentiation was analyzed using real-time polymerase chain reaction (PCR). Additionally, we evaluated the NF-M-positive cell rate 10 days after induction using immunofluorescent staining. RESULTS: Neural gene expression and the NF-M-positive cell rate were increased in hbMSCs under the 3G condition 10 days after induction. mRNA expression of RNA binding motif protein 4 (RBM4) and pyruvate kinase M 1 (PKM1) in the 3G condition was also higher than that in the 1G group. CONCLUSIONS: Hypergravity can enhance RBM4 and PKM1, promoting the neural differentiation of hbMSCs.
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spelling pubmed-98518672023-01-26 Hypergravity enhances RBM4 expression in human bone marrow-derived mesenchymal stem cells and accelerates their differentiation into neurons Teranishi, Masataka Kurose, Tomoyuki Nakagawa, Kei Kawahara, Yumi Yuge, Louis Regen Ther Original Article INTRODUCTION: The regulation of stem cell differentiation is important in determining the quality of transplanted cells in regenerative medicine. Physical stimuli are involved in regulating stem cell differentiation, and in particular, research on the regulation of differentiation using gravity is an attractive choice. We have shown that microgravity is useful for maintaining undifferentiated mesenchymal stem cells (MSCs). However, the effects of hypergravity on the differentiation of MSCs, especially on neural differentiation related to neural regeneration, have not been elucidated. METHODS: We induced neural differentiation of human bone marrow-derived MSCs (hbMSCs) for 10 days under normal gravity (1G) or hypergravity (3G) conditions using a gravity controller, Gravite®. HbMSCs were collected, and cell number and viability were measured 3 and 10 days after induction. RNA was also extracted from the collected hbMSCs, and the expression of neuron-associated genes and regulator markers of neural differentiation was analyzed using real-time polymerase chain reaction (PCR). Additionally, we evaluated the NF-M-positive cell rate 10 days after induction using immunofluorescent staining. RESULTS: Neural gene expression and the NF-M-positive cell rate were increased in hbMSCs under the 3G condition 10 days after induction. mRNA expression of RNA binding motif protein 4 (RBM4) and pyruvate kinase M 1 (PKM1) in the 3G condition was also higher than that in the 1G group. CONCLUSIONS: Hypergravity can enhance RBM4 and PKM1, promoting the neural differentiation of hbMSCs. Japanese Society for Regenerative Medicine 2023-01-16 /pmc/articles/PMC9851867/ /pubmed/36712961 http://dx.doi.org/10.1016/j.reth.2022.12.010 Text en © 2022 The Japanese Society for Regenerative Medicine. Production and hosting by Elsevier B.V. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Article
Teranishi, Masataka
Kurose, Tomoyuki
Nakagawa, Kei
Kawahara, Yumi
Yuge, Louis
Hypergravity enhances RBM4 expression in human bone marrow-derived mesenchymal stem cells and accelerates their differentiation into neurons
title Hypergravity enhances RBM4 expression in human bone marrow-derived mesenchymal stem cells and accelerates their differentiation into neurons
title_full Hypergravity enhances RBM4 expression in human bone marrow-derived mesenchymal stem cells and accelerates their differentiation into neurons
title_fullStr Hypergravity enhances RBM4 expression in human bone marrow-derived mesenchymal stem cells and accelerates their differentiation into neurons
title_full_unstemmed Hypergravity enhances RBM4 expression in human bone marrow-derived mesenchymal stem cells and accelerates their differentiation into neurons
title_short Hypergravity enhances RBM4 expression in human bone marrow-derived mesenchymal stem cells and accelerates their differentiation into neurons
title_sort hypergravity enhances rbm4 expression in human bone marrow-derived mesenchymal stem cells and accelerates their differentiation into neurons
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9851867/
https://www.ncbi.nlm.nih.gov/pubmed/36712961
http://dx.doi.org/10.1016/j.reth.2022.12.010
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