Semi-quantitative detection of pseudouridine modifications and type I/II hypermodifications in human mRNAs using direct long-read sequencing

Here, we develop and apply a semi-quantitative method for the high-confidence identification of pseudouridylated sites on mammalian mRNAs via direct long-read nanopore sequencing. A comparative analysis of a modification-free transcriptome reveals that the depth of coverage and specific k-mer sequen...

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Detalles Bibliográficos
Autores principales: Tavakoli, Sepideh, Nabizadeh, Mohammad, Makhamreh, Amr, Gamper, Howard, McCormick, Caroline A., Rezapour, Neda K., Hou, Ya-Ming, Wanunu, Meni, Rouhanifard, Sara H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9852470/
https://www.ncbi.nlm.nih.gov/pubmed/36658122
http://dx.doi.org/10.1038/s41467-023-35858-w

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