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Analyzing trogocytosis of T lymphocytes by flow cytometry and confocal microscopy

Here, we present a protocol to examine the mechanisms underlying the intercellular transfer of transmembrane molecules, termed trogocytosis, and the fate of transferred molecules. We describe the steps needed from T lymphocyte isolation, via co-culture with cells expressing the ligand of interest, t...

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Detalles Bibliográficos
Autores principales: Zink, Alicia, Zenke, Simon, Wiese, Teresa, Beyersdorf, Niklas, Lämmermann, Tim, Rohr, Jan C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9852653/
https://www.ncbi.nlm.nih.gov/pubmed/36638014
http://dx.doi.org/10.1016/j.xpro.2022.102013