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Analyzing trogocytosis of T lymphocytes by flow cytometry and confocal microscopy
Here, we present a protocol to examine the mechanisms underlying the intercellular transfer of transmembrane molecules, termed trogocytosis, and the fate of transferred molecules. We describe the steps needed from T lymphocyte isolation, via co-culture with cells expressing the ligand of interest, t...
Autores principales: | Zink, Alicia, Zenke, Simon, Wiese, Teresa, Beyersdorf, Niklas, Lämmermann, Tim, Rohr, Jan C. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9852653/ https://www.ncbi.nlm.nih.gov/pubmed/36638014 http://dx.doi.org/10.1016/j.xpro.2022.102013 |
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