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Simultaneous quantification method for eleutheroside B, eleutheroside E, chiisanoside, and sesamin using reverse-phase high-performance liquid chromatography coupled with ultraviolet detection and integrated pulsed amperometric detection

We developed a method combining ultraviolet (UV) detection and integrated pulsed amperometric detection (IPAD) to simultaneously analyze eleutheroside B, eleutheroside E, chiisanoside, and sesamin. The gradient elution system allowed complete separation of all target components within 35 min, and sh...

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Autores principales: Kim, YeaIn, Jung, Young-Ju, Yoon, Hae-Jee, Kwon, Ha-Jeong, Hong, Seon-Pyo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9852659/
https://www.ncbi.nlm.nih.gov/pubmed/36685467
http://dx.doi.org/10.1016/j.heliyon.2022.e12684
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author Kim, YeaIn
Jung, Young-Ju
Yoon, Hae-Jee
Kwon, Ha-Jeong
Hong, Seon-Pyo
author_facet Kim, YeaIn
Jung, Young-Ju
Yoon, Hae-Jee
Kwon, Ha-Jeong
Hong, Seon-Pyo
author_sort Kim, YeaIn
collection PubMed
description We developed a method combining ultraviolet (UV) detection and integrated pulsed amperometric detection (IPAD) to simultaneously analyze eleutheroside B, eleutheroside E, chiisanoside, and sesamin. The gradient elution system allowed complete separation of all target components within 35 min, and showed limits of detection of 0.006–0.020 μg/mL and limits of quantification of 0.018–0.050 μg/mL. The linear regression coefficients of determination were 0.9990–0.9998. All inter− and intra-day precision values were below 4.89%, and the average recoveries were 97.79–104.40%. The developed approach exhibits excellent reproducibility, sensitivity, and selectivity without requiring any complicated pre-treatment, and is therefore expected to be helpful as a tool for establishing appropriate content criteria for Acanthopanax species.
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spelling pubmed-98526592023-01-21 Simultaneous quantification method for eleutheroside B, eleutheroside E, chiisanoside, and sesamin using reverse-phase high-performance liquid chromatography coupled with ultraviolet detection and integrated pulsed amperometric detection Kim, YeaIn Jung, Young-Ju Yoon, Hae-Jee Kwon, Ha-Jeong Hong, Seon-Pyo Heliyon Research Article We developed a method combining ultraviolet (UV) detection and integrated pulsed amperometric detection (IPAD) to simultaneously analyze eleutheroside B, eleutheroside E, chiisanoside, and sesamin. The gradient elution system allowed complete separation of all target components within 35 min, and showed limits of detection of 0.006–0.020 μg/mL and limits of quantification of 0.018–0.050 μg/mL. The linear regression coefficients of determination were 0.9990–0.9998. All inter− and intra-day precision values were below 4.89%, and the average recoveries were 97.79–104.40%. The developed approach exhibits excellent reproducibility, sensitivity, and selectivity without requiring any complicated pre-treatment, and is therefore expected to be helpful as a tool for establishing appropriate content criteria for Acanthopanax species. Elsevier 2023-01-03 /pmc/articles/PMC9852659/ /pubmed/36685467 http://dx.doi.org/10.1016/j.heliyon.2022.e12684 Text en © 2023 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Research Article
Kim, YeaIn
Jung, Young-Ju
Yoon, Hae-Jee
Kwon, Ha-Jeong
Hong, Seon-Pyo
Simultaneous quantification method for eleutheroside B, eleutheroside E, chiisanoside, and sesamin using reverse-phase high-performance liquid chromatography coupled with ultraviolet detection and integrated pulsed amperometric detection
title Simultaneous quantification method for eleutheroside B, eleutheroside E, chiisanoside, and sesamin using reverse-phase high-performance liquid chromatography coupled with ultraviolet detection and integrated pulsed amperometric detection
title_full Simultaneous quantification method for eleutheroside B, eleutheroside E, chiisanoside, and sesamin using reverse-phase high-performance liquid chromatography coupled with ultraviolet detection and integrated pulsed amperometric detection
title_fullStr Simultaneous quantification method for eleutheroside B, eleutheroside E, chiisanoside, and sesamin using reverse-phase high-performance liquid chromatography coupled with ultraviolet detection and integrated pulsed amperometric detection
title_full_unstemmed Simultaneous quantification method for eleutheroside B, eleutheroside E, chiisanoside, and sesamin using reverse-phase high-performance liquid chromatography coupled with ultraviolet detection and integrated pulsed amperometric detection
title_short Simultaneous quantification method for eleutheroside B, eleutheroside E, chiisanoside, and sesamin using reverse-phase high-performance liquid chromatography coupled with ultraviolet detection and integrated pulsed amperometric detection
title_sort simultaneous quantification method for eleutheroside b, eleutheroside e, chiisanoside, and sesamin using reverse-phase high-performance liquid chromatography coupled with ultraviolet detection and integrated pulsed amperometric detection
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9852659/
https://www.ncbi.nlm.nih.gov/pubmed/36685467
http://dx.doi.org/10.1016/j.heliyon.2022.e12684
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