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Simultaneous quantification method for eleutheroside B, eleutheroside E, chiisanoside, and sesamin using reverse-phase high-performance liquid chromatography coupled with ultraviolet detection and integrated pulsed amperometric detection
We developed a method combining ultraviolet (UV) detection and integrated pulsed amperometric detection (IPAD) to simultaneously analyze eleutheroside B, eleutheroside E, chiisanoside, and sesamin. The gradient elution system allowed complete separation of all target components within 35 min, and sh...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9852659/ https://www.ncbi.nlm.nih.gov/pubmed/36685467 http://dx.doi.org/10.1016/j.heliyon.2022.e12684 |
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author | Kim, YeaIn Jung, Young-Ju Yoon, Hae-Jee Kwon, Ha-Jeong Hong, Seon-Pyo |
author_facet | Kim, YeaIn Jung, Young-Ju Yoon, Hae-Jee Kwon, Ha-Jeong Hong, Seon-Pyo |
author_sort | Kim, YeaIn |
collection | PubMed |
description | We developed a method combining ultraviolet (UV) detection and integrated pulsed amperometric detection (IPAD) to simultaneously analyze eleutheroside B, eleutheroside E, chiisanoside, and sesamin. The gradient elution system allowed complete separation of all target components within 35 min, and showed limits of detection of 0.006–0.020 μg/mL and limits of quantification of 0.018–0.050 μg/mL. The linear regression coefficients of determination were 0.9990–0.9998. All inter− and intra-day precision values were below 4.89%, and the average recoveries were 97.79–104.40%. The developed approach exhibits excellent reproducibility, sensitivity, and selectivity without requiring any complicated pre-treatment, and is therefore expected to be helpful as a tool for establishing appropriate content criteria for Acanthopanax species. |
format | Online Article Text |
id | pubmed-9852659 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-98526592023-01-21 Simultaneous quantification method for eleutheroside B, eleutheroside E, chiisanoside, and sesamin using reverse-phase high-performance liquid chromatography coupled with ultraviolet detection and integrated pulsed amperometric detection Kim, YeaIn Jung, Young-Ju Yoon, Hae-Jee Kwon, Ha-Jeong Hong, Seon-Pyo Heliyon Research Article We developed a method combining ultraviolet (UV) detection and integrated pulsed amperometric detection (IPAD) to simultaneously analyze eleutheroside B, eleutheroside E, chiisanoside, and sesamin. The gradient elution system allowed complete separation of all target components within 35 min, and showed limits of detection of 0.006–0.020 μg/mL and limits of quantification of 0.018–0.050 μg/mL. The linear regression coefficients of determination were 0.9990–0.9998. All inter− and intra-day precision values were below 4.89%, and the average recoveries were 97.79–104.40%. The developed approach exhibits excellent reproducibility, sensitivity, and selectivity without requiring any complicated pre-treatment, and is therefore expected to be helpful as a tool for establishing appropriate content criteria for Acanthopanax species. Elsevier 2023-01-03 /pmc/articles/PMC9852659/ /pubmed/36685467 http://dx.doi.org/10.1016/j.heliyon.2022.e12684 Text en © 2023 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Research Article Kim, YeaIn Jung, Young-Ju Yoon, Hae-Jee Kwon, Ha-Jeong Hong, Seon-Pyo Simultaneous quantification method for eleutheroside B, eleutheroside E, chiisanoside, and sesamin using reverse-phase high-performance liquid chromatography coupled with ultraviolet detection and integrated pulsed amperometric detection |
title | Simultaneous quantification method for eleutheroside B, eleutheroside E, chiisanoside, and sesamin using reverse-phase high-performance liquid chromatography coupled with ultraviolet detection and integrated pulsed amperometric detection |
title_full | Simultaneous quantification method for eleutheroside B, eleutheroside E, chiisanoside, and sesamin using reverse-phase high-performance liquid chromatography coupled with ultraviolet detection and integrated pulsed amperometric detection |
title_fullStr | Simultaneous quantification method for eleutheroside B, eleutheroside E, chiisanoside, and sesamin using reverse-phase high-performance liquid chromatography coupled with ultraviolet detection and integrated pulsed amperometric detection |
title_full_unstemmed | Simultaneous quantification method for eleutheroside B, eleutheroside E, chiisanoside, and sesamin using reverse-phase high-performance liquid chromatography coupled with ultraviolet detection and integrated pulsed amperometric detection |
title_short | Simultaneous quantification method for eleutheroside B, eleutheroside E, chiisanoside, and sesamin using reverse-phase high-performance liquid chromatography coupled with ultraviolet detection and integrated pulsed amperometric detection |
title_sort | simultaneous quantification method for eleutheroside b, eleutheroside e, chiisanoside, and sesamin using reverse-phase high-performance liquid chromatography coupled with ultraviolet detection and integrated pulsed amperometric detection |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9852659/ https://www.ncbi.nlm.nih.gov/pubmed/36685467 http://dx.doi.org/10.1016/j.heliyon.2022.e12684 |
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