Protocol for the induction of hindlimb ischemia and isolation of muscle endothelial cells in mice

A mouse model of hindlimb ischemia is an important tool for studying diverse therapeutic approaches for vascularization with high surgical success and low mortality rates. Here, we present a protocol for the induction of hindlimb ischemia in mice, including the surgery procedure and steps to analyze...

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Autores principales: Kim, Min Seong, Vital, Shantel, Park, Changwon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9852691/
https://www.ncbi.nlm.nih.gov/pubmed/36638013
http://dx.doi.org/10.1016/j.xpro.2022.102017
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author Kim, Min Seong
Vital, Shantel
Park, Changwon
author_facet Kim, Min Seong
Vital, Shantel
Park, Changwon
author_sort Kim, Min Seong
collection PubMed
description A mouse model of hindlimb ischemia is an important tool for studying diverse therapeutic approaches for vascularization with high surgical success and low mortality rates. Here, we present a protocol for the induction of hindlimb ischemia in mice, including the surgery procedure and steps to analyze blood perfusion in the ischemic area using a laser speckle contrast analyzer. We also detail the isolation of endothelial cells from thigh muscles using flow cytometry after ischemic surgery. For complete details on the use and execution of this protocol, please refer to Park et al. (2016).(1)
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spelling pubmed-98526912023-01-21 Protocol for the induction of hindlimb ischemia and isolation of muscle endothelial cells in mice Kim, Min Seong Vital, Shantel Park, Changwon STAR Protoc Protocol A mouse model of hindlimb ischemia is an important tool for studying diverse therapeutic approaches for vascularization with high surgical success and low mortality rates. Here, we present a protocol for the induction of hindlimb ischemia in mice, including the surgery procedure and steps to analyze blood perfusion in the ischemic area using a laser speckle contrast analyzer. We also detail the isolation of endothelial cells from thigh muscles using flow cytometry after ischemic surgery. For complete details on the use and execution of this protocol, please refer to Park et al. (2016).(1) Elsevier 2023-01-12 /pmc/articles/PMC9852691/ /pubmed/36638013 http://dx.doi.org/10.1016/j.xpro.2022.102017 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Kim, Min Seong
Vital, Shantel
Park, Changwon
Protocol for the induction of hindlimb ischemia and isolation of muscle endothelial cells in mice
title Protocol for the induction of hindlimb ischemia and isolation of muscle endothelial cells in mice
title_full Protocol for the induction of hindlimb ischemia and isolation of muscle endothelial cells in mice
title_fullStr Protocol for the induction of hindlimb ischemia and isolation of muscle endothelial cells in mice
title_full_unstemmed Protocol for the induction of hindlimb ischemia and isolation of muscle endothelial cells in mice
title_short Protocol for the induction of hindlimb ischemia and isolation of muscle endothelial cells in mice
title_sort protocol for the induction of hindlimb ischemia and isolation of muscle endothelial cells in mice
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9852691/
https://www.ncbi.nlm.nih.gov/pubmed/36638013
http://dx.doi.org/10.1016/j.xpro.2022.102017
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