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Structure and metabolic potential of the prokaryotic communities from the hydrothermal system of Paleochori Bay, Milos, Greece

INTRODUCTION: Shallow hydrothermal systems share many characteristics with their deep-sea counterparts, but their accessibility facilitates their study. One of the most studied shallow hydrothermal vent fields lies at Paleochori Bay off the coast of Milos in the Aegean Sea (Greece). It has been stud...

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Autores principales: Le Moine Bauer, Sven, Lu, Guang-Sin, Goulaouic, Steven, Puzenat, Valentine, Schouw, Anders, Barreyre, Thibaut, Pawlowsky-Glahn, Vera, Egozcue, Juan José, Martelat, Jean-Emmanuel, Escartin, Javier, Amend, Jan P., Nomikou, Paraskevi, Vlasopoulos, Othonas, Polymenakou, Paraskevi, Jørgensen, Steffen Leth
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9852839/
https://www.ncbi.nlm.nih.gov/pubmed/36687571
http://dx.doi.org/10.3389/fmicb.2022.1060168
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author Le Moine Bauer, Sven
Lu, Guang-Sin
Goulaouic, Steven
Puzenat, Valentine
Schouw, Anders
Barreyre, Thibaut
Pawlowsky-Glahn, Vera
Egozcue, Juan José
Martelat, Jean-Emmanuel
Escartin, Javier
Amend, Jan P.
Nomikou, Paraskevi
Vlasopoulos, Othonas
Polymenakou, Paraskevi
Jørgensen, Steffen Leth
author_facet Le Moine Bauer, Sven
Lu, Guang-Sin
Goulaouic, Steven
Puzenat, Valentine
Schouw, Anders
Barreyre, Thibaut
Pawlowsky-Glahn, Vera
Egozcue, Juan José
Martelat, Jean-Emmanuel
Escartin, Javier
Amend, Jan P.
Nomikou, Paraskevi
Vlasopoulos, Othonas
Polymenakou, Paraskevi
Jørgensen, Steffen Leth
author_sort Le Moine Bauer, Sven
collection PubMed
description INTRODUCTION: Shallow hydrothermal systems share many characteristics with their deep-sea counterparts, but their accessibility facilitates their study. One of the most studied shallow hydrothermal vent fields lies at Paleochori Bay off the coast of Milos in the Aegean Sea (Greece). It has been studied through extensive mapping and its physical and chemical processes have been characterized over the past decades. However, a thorough description of the microbial communities inhabiting the bay is still missing. METHODS: We present the first in-depth characterization of the prokaryotic communities of Paleochori Bay by sampling eight different seafloor types that are distributed along the entire gradient of hydrothermal influence. We used deep sequencing of the 16S rRNA marker gene and complemented the analysis with qPCR quantification of the 16S rRNA gene and several functional genes to gain insights into the metabolic potential of the communities. RESULTS: We found that the microbiome of the bay is strongly influenced by the hydrothermal venting, with a succession of various groups dominating the sediments from the coldest to the warmest zones. Prokaryotic diversity and abundance decrease with increasing temperature, and thermophilic archaea overtake the community. DISCUSSION: Relevant geochemical cycles of the Bay are discussed. This study expands our limited understanding of subsurface microbial communities in acidic shallow-sea hydrothermal systems and the contribution of their microbial activity to biogeochemical cycling.
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spelling pubmed-98528392023-01-21 Structure and metabolic potential of the prokaryotic communities from the hydrothermal system of Paleochori Bay, Milos, Greece Le Moine Bauer, Sven Lu, Guang-Sin Goulaouic, Steven Puzenat, Valentine Schouw, Anders Barreyre, Thibaut Pawlowsky-Glahn, Vera Egozcue, Juan José Martelat, Jean-Emmanuel Escartin, Javier Amend, Jan P. Nomikou, Paraskevi Vlasopoulos, Othonas Polymenakou, Paraskevi Jørgensen, Steffen Leth Front Microbiol Microbiology INTRODUCTION: Shallow hydrothermal systems share many characteristics with their deep-sea counterparts, but their accessibility facilitates their study. One of the most studied shallow hydrothermal vent fields lies at Paleochori Bay off the coast of Milos in the Aegean Sea (Greece). It has been studied through extensive mapping and its physical and chemical processes have been characterized over the past decades. However, a thorough description of the microbial communities inhabiting the bay is still missing. METHODS: We present the first in-depth characterization of the prokaryotic communities of Paleochori Bay by sampling eight different seafloor types that are distributed along the entire gradient of hydrothermal influence. We used deep sequencing of the 16S rRNA marker gene and complemented the analysis with qPCR quantification of the 16S rRNA gene and several functional genes to gain insights into the metabolic potential of the communities. RESULTS: We found that the microbiome of the bay is strongly influenced by the hydrothermal venting, with a succession of various groups dominating the sediments from the coldest to the warmest zones. Prokaryotic diversity and abundance decrease with increasing temperature, and thermophilic archaea overtake the community. DISCUSSION: Relevant geochemical cycles of the Bay are discussed. This study expands our limited understanding of subsurface microbial communities in acidic shallow-sea hydrothermal systems and the contribution of their microbial activity to biogeochemical cycling. Frontiers Media S.A. 2023-01-06 /pmc/articles/PMC9852839/ /pubmed/36687571 http://dx.doi.org/10.3389/fmicb.2022.1060168 Text en Copyright © 2023 Le Moine Bauer, Lu, Goulaouic, Puzenat, Schouw, Barreyre, Pawlowsky-Glahn, Egozcue, Martelat, Escartin, Amend, Nomikou, Vlasopoulos, Polymenakou and Jørgensen. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Le Moine Bauer, Sven
Lu, Guang-Sin
Goulaouic, Steven
Puzenat, Valentine
Schouw, Anders
Barreyre, Thibaut
Pawlowsky-Glahn, Vera
Egozcue, Juan José
Martelat, Jean-Emmanuel
Escartin, Javier
Amend, Jan P.
Nomikou, Paraskevi
Vlasopoulos, Othonas
Polymenakou, Paraskevi
Jørgensen, Steffen Leth
Structure and metabolic potential of the prokaryotic communities from the hydrothermal system of Paleochori Bay, Milos, Greece
title Structure and metabolic potential of the prokaryotic communities from the hydrothermal system of Paleochori Bay, Milos, Greece
title_full Structure and metabolic potential of the prokaryotic communities from the hydrothermal system of Paleochori Bay, Milos, Greece
title_fullStr Structure and metabolic potential of the prokaryotic communities from the hydrothermal system of Paleochori Bay, Milos, Greece
title_full_unstemmed Structure and metabolic potential of the prokaryotic communities from the hydrothermal system of Paleochori Bay, Milos, Greece
title_short Structure and metabolic potential of the prokaryotic communities from the hydrothermal system of Paleochori Bay, Milos, Greece
title_sort structure and metabolic potential of the prokaryotic communities from the hydrothermal system of paleochori bay, milos, greece
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9852839/
https://www.ncbi.nlm.nih.gov/pubmed/36687571
http://dx.doi.org/10.3389/fmicb.2022.1060168
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