Comprehensive whole genome sequencing with hybrid assembly of multi-drug resistant Candida albicans isolate causing cerebral abscess

Comprehensive whole genome sequencing (WGS) with hybrid assembly of a multi-drug resistant (MDR) Candida albicans (CA) isolate causing cerebral abscess was performed using Illumina paired end and Oxford Nanopore long read technologies. The innovative technologies utilized here enabled us to resolve...

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Detalles Bibliográficos
Autores principales: Kumaraswamy, Monika, Coady, Alison, Szubin, Richard, Martin, Thomas CS, Palsson, Bernhard, Nizet, Victor, Monk, Jonathan M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9852921/
https://www.ncbi.nlm.nih.gov/pubmed/36685102
http://dx.doi.org/10.1016/j.crmicr.2023.100180
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author Kumaraswamy, Monika
Coady, Alison
Szubin, Richard
Martin, Thomas CS
Palsson, Bernhard
Nizet, Victor
Monk, Jonathan M.
author_facet Kumaraswamy, Monika
Coady, Alison
Szubin, Richard
Martin, Thomas CS
Palsson, Bernhard
Nizet, Victor
Monk, Jonathan M.
author_sort Kumaraswamy, Monika
collection PubMed
description Comprehensive whole genome sequencing (WGS) with hybrid assembly of a multi-drug resistant (MDR) Candida albicans (CA) isolate causing cerebral abscess was performed using Illumina paired end and Oxford Nanopore long read technologies. The innovative technologies utilized here enabled us to resolve fragmented assemblies, and implement comprehensive and detailed genomic analyses involved in antifungal resistance of Candida spp. Functionally important genes (MDR1, CDR2 and SQN2) involved in antifungal resistance were identified and a phylogenetic analysis of the clinical isolate was performed. Additionally, our clinical isolate was found to share 4 single nucleotide polymorphisms with two other sequenced strains of MDR C. auris (381 and 386) including translation elongation factor EF1α and EF3, ATPase activity associated proteins, and the lysine tRNA ligase.
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spelling pubmed-98529212023-01-21 Comprehensive whole genome sequencing with hybrid assembly of multi-drug resistant Candida albicans isolate causing cerebral abscess Kumaraswamy, Monika Coady, Alison Szubin, Richard Martin, Thomas CS Palsson, Bernhard Nizet, Victor Monk, Jonathan M. Curr Res Microb Sci Short Communication Comprehensive whole genome sequencing (WGS) with hybrid assembly of a multi-drug resistant (MDR) Candida albicans (CA) isolate causing cerebral abscess was performed using Illumina paired end and Oxford Nanopore long read technologies. The innovative technologies utilized here enabled us to resolve fragmented assemblies, and implement comprehensive and detailed genomic analyses involved in antifungal resistance of Candida spp. Functionally important genes (MDR1, CDR2 and SQN2) involved in antifungal resistance were identified and a phylogenetic analysis of the clinical isolate was performed. Additionally, our clinical isolate was found to share 4 single nucleotide polymorphisms with two other sequenced strains of MDR C. auris (381 and 386) including translation elongation factor EF1α and EF3, ATPase activity associated proteins, and the lysine tRNA ligase. Elsevier 2023-01-09 /pmc/articles/PMC9852921/ /pubmed/36685102 http://dx.doi.org/10.1016/j.crmicr.2023.100180 Text en Published by Elsevier B.V. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Short Communication
Kumaraswamy, Monika
Coady, Alison
Szubin, Richard
Martin, Thomas CS
Palsson, Bernhard
Nizet, Victor
Monk, Jonathan M.
Comprehensive whole genome sequencing with hybrid assembly of multi-drug resistant Candida albicans isolate causing cerebral abscess
title Comprehensive whole genome sequencing with hybrid assembly of multi-drug resistant Candida albicans isolate causing cerebral abscess
title_full Comprehensive whole genome sequencing with hybrid assembly of multi-drug resistant Candida albicans isolate causing cerebral abscess
title_fullStr Comprehensive whole genome sequencing with hybrid assembly of multi-drug resistant Candida albicans isolate causing cerebral abscess
title_full_unstemmed Comprehensive whole genome sequencing with hybrid assembly of multi-drug resistant Candida albicans isolate causing cerebral abscess
title_short Comprehensive whole genome sequencing with hybrid assembly of multi-drug resistant Candida albicans isolate causing cerebral abscess
title_sort comprehensive whole genome sequencing with hybrid assembly of multi-drug resistant candida albicans isolate causing cerebral abscess
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9852921/
https://www.ncbi.nlm.nih.gov/pubmed/36685102
http://dx.doi.org/10.1016/j.crmicr.2023.100180
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