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Comprehensive whole genome sequencing with hybrid assembly of multi-drug resistant Candida albicans isolate causing cerebral abscess
Comprehensive whole genome sequencing (WGS) with hybrid assembly of a multi-drug resistant (MDR) Candida albicans (CA) isolate causing cerebral abscess was performed using Illumina paired end and Oxford Nanopore long read technologies. The innovative technologies utilized here enabled us to resolve...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9852921/ https://www.ncbi.nlm.nih.gov/pubmed/36685102 http://dx.doi.org/10.1016/j.crmicr.2023.100180 |
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author | Kumaraswamy, Monika Coady, Alison Szubin, Richard Martin, Thomas CS Palsson, Bernhard Nizet, Victor Monk, Jonathan M. |
author_facet | Kumaraswamy, Monika Coady, Alison Szubin, Richard Martin, Thomas CS Palsson, Bernhard Nizet, Victor Monk, Jonathan M. |
author_sort | Kumaraswamy, Monika |
collection | PubMed |
description | Comprehensive whole genome sequencing (WGS) with hybrid assembly of a multi-drug resistant (MDR) Candida albicans (CA) isolate causing cerebral abscess was performed using Illumina paired end and Oxford Nanopore long read technologies. The innovative technologies utilized here enabled us to resolve fragmented assemblies, and implement comprehensive and detailed genomic analyses involved in antifungal resistance of Candida spp. Functionally important genes (MDR1, CDR2 and SQN2) involved in antifungal resistance were identified and a phylogenetic analysis of the clinical isolate was performed. Additionally, our clinical isolate was found to share 4 single nucleotide polymorphisms with two other sequenced strains of MDR C. auris (381 and 386) including translation elongation factor EF1α and EF3, ATPase activity associated proteins, and the lysine tRNA ligase. |
format | Online Article Text |
id | pubmed-9852921 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-98529212023-01-21 Comprehensive whole genome sequencing with hybrid assembly of multi-drug resistant Candida albicans isolate causing cerebral abscess Kumaraswamy, Monika Coady, Alison Szubin, Richard Martin, Thomas CS Palsson, Bernhard Nizet, Victor Monk, Jonathan M. Curr Res Microb Sci Short Communication Comprehensive whole genome sequencing (WGS) with hybrid assembly of a multi-drug resistant (MDR) Candida albicans (CA) isolate causing cerebral abscess was performed using Illumina paired end and Oxford Nanopore long read technologies. The innovative technologies utilized here enabled us to resolve fragmented assemblies, and implement comprehensive and detailed genomic analyses involved in antifungal resistance of Candida spp. Functionally important genes (MDR1, CDR2 and SQN2) involved in antifungal resistance were identified and a phylogenetic analysis of the clinical isolate was performed. Additionally, our clinical isolate was found to share 4 single nucleotide polymorphisms with two other sequenced strains of MDR C. auris (381 and 386) including translation elongation factor EF1α and EF3, ATPase activity associated proteins, and the lysine tRNA ligase. Elsevier 2023-01-09 /pmc/articles/PMC9852921/ /pubmed/36685102 http://dx.doi.org/10.1016/j.crmicr.2023.100180 Text en Published by Elsevier B.V. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Short Communication Kumaraswamy, Monika Coady, Alison Szubin, Richard Martin, Thomas CS Palsson, Bernhard Nizet, Victor Monk, Jonathan M. Comprehensive whole genome sequencing with hybrid assembly of multi-drug resistant Candida albicans isolate causing cerebral abscess |
title | Comprehensive whole genome sequencing with hybrid assembly of multi-drug resistant Candida albicans isolate causing cerebral abscess |
title_full | Comprehensive whole genome sequencing with hybrid assembly of multi-drug resistant Candida albicans isolate causing cerebral abscess |
title_fullStr | Comprehensive whole genome sequencing with hybrid assembly of multi-drug resistant Candida albicans isolate causing cerebral abscess |
title_full_unstemmed | Comprehensive whole genome sequencing with hybrid assembly of multi-drug resistant Candida albicans isolate causing cerebral abscess |
title_short | Comprehensive whole genome sequencing with hybrid assembly of multi-drug resistant Candida albicans isolate causing cerebral abscess |
title_sort | comprehensive whole genome sequencing with hybrid assembly of multi-drug resistant candida albicans isolate causing cerebral abscess |
topic | Short Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9852921/ https://www.ncbi.nlm.nih.gov/pubmed/36685102 http://dx.doi.org/10.1016/j.crmicr.2023.100180 |
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