Cargando…

Comparative transcriptome, digital gene expression and proteome profiling analyses provide insights into the brachyurization from the megalopa to the first juvenile in Eriocheir sinensis

Eriocheir sinensis larva normally experiences 11 stages. The reduced abdomen folded beneath the thorax is the most prominent characteristic of morphological alteration from megalopa to juvenile crab. Up to date, the molecular mechanisms of brachyurization remain a mystery. Here, transcriptome librar...

Descripción completa

Detalles Bibliográficos
Autores principales: Yang, Yunxia, Jin, Fangcao, Liu, Wanyi, Huo, Guangming, Zhou, Feng, Yan, Jie, Zhou, Kaiya, Li, Peng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9853305/
https://www.ncbi.nlm.nih.gov/pubmed/36685450
http://dx.doi.org/10.1016/j.heliyon.2022.e12736
_version_ 1784872865682685952
author Yang, Yunxia
Jin, Fangcao
Liu, Wanyi
Huo, Guangming
Zhou, Feng
Yan, Jie
Zhou, Kaiya
Li, Peng
author_facet Yang, Yunxia
Jin, Fangcao
Liu, Wanyi
Huo, Guangming
Zhou, Feng
Yan, Jie
Zhou, Kaiya
Li, Peng
author_sort Yang, Yunxia
collection PubMed
description Eriocheir sinensis larva normally experiences 11 stages. The reduced abdomen folded beneath the thorax is the most prominent characteristic of morphological alteration from megalopa to juvenile crab. Up to date, the molecular mechanisms of brachyurization remain a mystery. Here, transcriptome library, digital gene expression (DGE) libraries and proteome libraries at two developmental stages [the megalopa stage of E. sinensis (stage M) and the first stage of juvenile crab (stage J1)] of the Chinese mitten crab larva were constructed for RNA sequencing and iTRAQ approaches followed by bioinformatics analysis, respectively. In total, 1106 genes and 871 proteins were differentially expressed between the stage M and stage J1. Moreover, several important pathways were identified, including biosynthesis of secondary metabolites, metabolic pathways, focal adhesion, and some disease pathways. Besides, muscle contraction, oxidative phosphorylation, calcium signaling, PI3K-Akt, DNA replication pathway, and integrin signaling pathway also had important functions in brachyurization process. Furthermore, the components, actin, actin-related protein, collagens, filamin-A/B, laminin, integrins, paxillin, and fibronectin had up-regulated expression levels in M stage compared to J1 stage.
format Online
Article
Text
id pubmed-9853305
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-98533052023-01-21 Comparative transcriptome, digital gene expression and proteome profiling analyses provide insights into the brachyurization from the megalopa to the first juvenile in Eriocheir sinensis Yang, Yunxia Jin, Fangcao Liu, Wanyi Huo, Guangming Zhou, Feng Yan, Jie Zhou, Kaiya Li, Peng Heliyon Research Article Eriocheir sinensis larva normally experiences 11 stages. The reduced abdomen folded beneath the thorax is the most prominent characteristic of morphological alteration from megalopa to juvenile crab. Up to date, the molecular mechanisms of brachyurization remain a mystery. Here, transcriptome library, digital gene expression (DGE) libraries and proteome libraries at two developmental stages [the megalopa stage of E. sinensis (stage M) and the first stage of juvenile crab (stage J1)] of the Chinese mitten crab larva were constructed for RNA sequencing and iTRAQ approaches followed by bioinformatics analysis, respectively. In total, 1106 genes and 871 proteins were differentially expressed between the stage M and stage J1. Moreover, several important pathways were identified, including biosynthesis of secondary metabolites, metabolic pathways, focal adhesion, and some disease pathways. Besides, muscle contraction, oxidative phosphorylation, calcium signaling, PI3K-Akt, DNA replication pathway, and integrin signaling pathway also had important functions in brachyurization process. Furthermore, the components, actin, actin-related protein, collagens, filamin-A/B, laminin, integrins, paxillin, and fibronectin had up-regulated expression levels in M stage compared to J1 stage. Elsevier 2023-01-07 /pmc/articles/PMC9853305/ /pubmed/36685450 http://dx.doi.org/10.1016/j.heliyon.2022.e12736 Text en © 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Yang, Yunxia
Jin, Fangcao
Liu, Wanyi
Huo, Guangming
Zhou, Feng
Yan, Jie
Zhou, Kaiya
Li, Peng
Comparative transcriptome, digital gene expression and proteome profiling analyses provide insights into the brachyurization from the megalopa to the first juvenile in Eriocheir sinensis
title Comparative transcriptome, digital gene expression and proteome profiling analyses provide insights into the brachyurization from the megalopa to the first juvenile in Eriocheir sinensis
title_full Comparative transcriptome, digital gene expression and proteome profiling analyses provide insights into the brachyurization from the megalopa to the first juvenile in Eriocheir sinensis
title_fullStr Comparative transcriptome, digital gene expression and proteome profiling analyses provide insights into the brachyurization from the megalopa to the first juvenile in Eriocheir sinensis
title_full_unstemmed Comparative transcriptome, digital gene expression and proteome profiling analyses provide insights into the brachyurization from the megalopa to the first juvenile in Eriocheir sinensis
title_short Comparative transcriptome, digital gene expression and proteome profiling analyses provide insights into the brachyurization from the megalopa to the first juvenile in Eriocheir sinensis
title_sort comparative transcriptome, digital gene expression and proteome profiling analyses provide insights into the brachyurization from the megalopa to the first juvenile in eriocheir sinensis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9853305/
https://www.ncbi.nlm.nih.gov/pubmed/36685450
http://dx.doi.org/10.1016/j.heliyon.2022.e12736
work_keys_str_mv AT yangyunxia comparativetranscriptomedigitalgeneexpressionandproteomeprofilinganalysesprovideinsightsintothebrachyurizationfromthemegalopatothefirstjuvenileineriocheirsinensis
AT jinfangcao comparativetranscriptomedigitalgeneexpressionandproteomeprofilinganalysesprovideinsightsintothebrachyurizationfromthemegalopatothefirstjuvenileineriocheirsinensis
AT liuwanyi comparativetranscriptomedigitalgeneexpressionandproteomeprofilinganalysesprovideinsightsintothebrachyurizationfromthemegalopatothefirstjuvenileineriocheirsinensis
AT huoguangming comparativetranscriptomedigitalgeneexpressionandproteomeprofilinganalysesprovideinsightsintothebrachyurizationfromthemegalopatothefirstjuvenileineriocheirsinensis
AT zhoufeng comparativetranscriptomedigitalgeneexpressionandproteomeprofilinganalysesprovideinsightsintothebrachyurizationfromthemegalopatothefirstjuvenileineriocheirsinensis
AT yanjie comparativetranscriptomedigitalgeneexpressionandproteomeprofilinganalysesprovideinsightsintothebrachyurizationfromthemegalopatothefirstjuvenileineriocheirsinensis
AT zhoukaiya comparativetranscriptomedigitalgeneexpressionandproteomeprofilinganalysesprovideinsightsintothebrachyurizationfromthemegalopatothefirstjuvenileineriocheirsinensis
AT lipeng comparativetranscriptomedigitalgeneexpressionandproteomeprofilinganalysesprovideinsightsintothebrachyurizationfromthemegalopatothefirstjuvenileineriocheirsinensis