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Western diet augments metabolic and arterial dysfunction in a sex-specific manner in outbred, genetically diverse mice

Western diet (WD), characterized by excess saturated fat and sugar intake, is a major contributor to obesity and metabolic and arterial dysfunction in humans. However, these phenotypes are not consistently observed in traditional inbred, genetically identical mice. Therefore, we sought to determine...

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Autores principales: Zheng, Xiangyu, Li, Zhuoxin, Berg Sen, Jennifer, Samarah, Luaye, Deacon, Christina S., Bernardo, Joseph, Machin, Daniel R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9853899/
https://www.ncbi.nlm.nih.gov/pubmed/36687716
http://dx.doi.org/10.3389/fnut.2022.1090023
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author Zheng, Xiangyu
Li, Zhuoxin
Berg Sen, Jennifer
Samarah, Luaye
Deacon, Christina S.
Bernardo, Joseph
Machin, Daniel R.
author_facet Zheng, Xiangyu
Li, Zhuoxin
Berg Sen, Jennifer
Samarah, Luaye
Deacon, Christina S.
Bernardo, Joseph
Machin, Daniel R.
author_sort Zheng, Xiangyu
collection PubMed
description Western diet (WD), characterized by excess saturated fat and sugar intake, is a major contributor to obesity and metabolic and arterial dysfunction in humans. However, these phenotypes are not consistently observed in traditional inbred, genetically identical mice. Therefore, we sought to determine the effects of WD on visceral adiposity and metabolic/arterial function in UM-HET3 mice, an outbred, genetically diverse strain of mice. Male and female UM-HET3 mice underwent normal chow (NC) or WD for 12 weeks. Body mass and visceral adiposity were higher in WD compared to NC (P < 0.05). Female WD mice had greater visceral adiposity than male WD mice (P < 0.05). The results of glucose and insulin tolerance tests demonstrated that metabolic function was lower in WD compared to NC mice (P < 0.05). Metabolic dysfunction in WD as was driven by male mice, as metabolic function in female WD mice was unchanged (P > 0.05). Systolic blood pressure (BP) and aortic stiffness were increased in WD after 2 weeks compared to baseline and continued to increase through week 12 (P < 0.05). Systolic BP and aortic stiffness were higher from weeks 2-12 in WD compared to NC (P < 0.05). Aortic collagen content was higher in WD compared to NC (P < 0.05). Carotid artery endothelium-dependent dilation was lower in WD compared to NC (P < 0.05). These data suggest sex-related differences in visceral adiposity and metabolic dysfunction in response to WD. Despite this, arterial dysfunction was similar in male and female WD mice, indicating this model may provide unique translational insight into similar sex-related observations in humans that consume WD.
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spelling pubmed-98538992023-01-21 Western diet augments metabolic and arterial dysfunction in a sex-specific manner in outbred, genetically diverse mice Zheng, Xiangyu Li, Zhuoxin Berg Sen, Jennifer Samarah, Luaye Deacon, Christina S. Bernardo, Joseph Machin, Daniel R. Front Nutr Nutrition Western diet (WD), characterized by excess saturated fat and sugar intake, is a major contributor to obesity and metabolic and arterial dysfunction in humans. However, these phenotypes are not consistently observed in traditional inbred, genetically identical mice. Therefore, we sought to determine the effects of WD on visceral adiposity and metabolic/arterial function in UM-HET3 mice, an outbred, genetically diverse strain of mice. Male and female UM-HET3 mice underwent normal chow (NC) or WD for 12 weeks. Body mass and visceral adiposity were higher in WD compared to NC (P < 0.05). Female WD mice had greater visceral adiposity than male WD mice (P < 0.05). The results of glucose and insulin tolerance tests demonstrated that metabolic function was lower in WD compared to NC mice (P < 0.05). Metabolic dysfunction in WD as was driven by male mice, as metabolic function in female WD mice was unchanged (P > 0.05). Systolic blood pressure (BP) and aortic stiffness were increased in WD after 2 weeks compared to baseline and continued to increase through week 12 (P < 0.05). Systolic BP and aortic stiffness were higher from weeks 2-12 in WD compared to NC (P < 0.05). Aortic collagen content was higher in WD compared to NC (P < 0.05). Carotid artery endothelium-dependent dilation was lower in WD compared to NC (P < 0.05). These data suggest sex-related differences in visceral adiposity and metabolic dysfunction in response to WD. Despite this, arterial dysfunction was similar in male and female WD mice, indicating this model may provide unique translational insight into similar sex-related observations in humans that consume WD. Frontiers Media S.A. 2023-01-06 /pmc/articles/PMC9853899/ /pubmed/36687716 http://dx.doi.org/10.3389/fnut.2022.1090023 Text en Copyright © 2023 Zheng, Li, Berg Sen, Samarah, Deacon, Bernardo and Machin. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Nutrition
Zheng, Xiangyu
Li, Zhuoxin
Berg Sen, Jennifer
Samarah, Luaye
Deacon, Christina S.
Bernardo, Joseph
Machin, Daniel R.
Western diet augments metabolic and arterial dysfunction in a sex-specific manner in outbred, genetically diverse mice
title Western diet augments metabolic and arterial dysfunction in a sex-specific manner in outbred, genetically diverse mice
title_full Western diet augments metabolic and arterial dysfunction in a sex-specific manner in outbred, genetically diverse mice
title_fullStr Western diet augments metabolic and arterial dysfunction in a sex-specific manner in outbred, genetically diverse mice
title_full_unstemmed Western diet augments metabolic and arterial dysfunction in a sex-specific manner in outbred, genetically diverse mice
title_short Western diet augments metabolic and arterial dysfunction in a sex-specific manner in outbred, genetically diverse mice
title_sort western diet augments metabolic and arterial dysfunction in a sex-specific manner in outbred, genetically diverse mice
topic Nutrition
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9853899/
https://www.ncbi.nlm.nih.gov/pubmed/36687716
http://dx.doi.org/10.3389/fnut.2022.1090023
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