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Increased Myosin light chain 9 expression during Kawasaki disease vasculitis

INTRODUCTION: Kawasaki disease (KD) is an acute systemic vasculitis that predominantly afflicts children. KD development is known to be associated with an aberrant immune response and abnormal platelet activation, however its etiology is still largely unknown. Myosin light chain 9 (Myl9) is known to...

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Autores principales: Kobayashi, Hironobu, Kimura, Motoko Y., Hasegawa, Ichita, Suganuma, Eisuke, Ikehara, Yuzuru, Azuma, Kazuhiko, Ito, Toshihiro, Ebata, Ryota, Kurashima, Yosuke, Kawasaki, Yohei, Shiko, Yuki, Saito, Naoki, Iwase, Hirotaro, Lee, Youngho, Noval Rivas, Magali, Arditi, Moshe, Zuka, Masahiko, Hamada, Hiromichi, Nakayama, Toshinori
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9853906/
https://www.ncbi.nlm.nih.gov/pubmed/36685558
http://dx.doi.org/10.3389/fimmu.2022.1036672
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author Kobayashi, Hironobu
Kimura, Motoko Y.
Hasegawa, Ichita
Suganuma, Eisuke
Ikehara, Yuzuru
Azuma, Kazuhiko
Ito, Toshihiro
Ebata, Ryota
Kurashima, Yosuke
Kawasaki, Yohei
Shiko, Yuki
Saito, Naoki
Iwase, Hirotaro
Lee, Youngho
Noval Rivas, Magali
Arditi, Moshe
Zuka, Masahiko
Hamada, Hiromichi
Nakayama, Toshinori
author_facet Kobayashi, Hironobu
Kimura, Motoko Y.
Hasegawa, Ichita
Suganuma, Eisuke
Ikehara, Yuzuru
Azuma, Kazuhiko
Ito, Toshihiro
Ebata, Ryota
Kurashima, Yosuke
Kawasaki, Yohei
Shiko, Yuki
Saito, Naoki
Iwase, Hirotaro
Lee, Youngho
Noval Rivas, Magali
Arditi, Moshe
Zuka, Masahiko
Hamada, Hiromichi
Nakayama, Toshinori
author_sort Kobayashi, Hironobu
collection PubMed
description INTRODUCTION: Kawasaki disease (KD) is an acute systemic vasculitis that predominantly afflicts children. KD development is known to be associated with an aberrant immune response and abnormal platelet activation, however its etiology is still largely unknown. Myosin light chain 9 (Myl9) is known to regulate cellular contractility of both non-muscle and smooth muscle cells, and can be released from platelets, whereas any relations of Myl9 expression to KD vasculitis have not been examined. METHODS: Plasma Myl9 concentrations in KD patients and children with febrile illness were measured and associated with KD clinical course and prognosis. Myl9 release from platelets in KD patients was also evaluated in vitro. Myl9 expression was determined in coronary arteries from Lactobacillus casei cell wall extract (LCWE)-injected mice that develop experimental KD vasculitis, as well as in cardiac tissues obtained at autopsy from KD patients. RESULTS AND DISCUSSION: Plasma Myl9 levels were significantly higher in KD patients during the acute phase compared with healthy controls or patients with other febrile illnesses, declined following IVIG therapy in IVIG-responders but not in non-responders. In vitro, platelets from KD patients released Myl9 independently of thrombin stimulation. In the LCWE-injected mice, Myl9 was detected in cardiac tissue at an early stage before inflammatory cell infiltration was observed. In tissues obtained at autopsy from KD patients, the highest Myl9 expression was observed in thrombi during the acute phase and in the intima and adventitia of coronary arteries during the chronic phase. Thus, our studies show that Myl9 expression is significantly increased during KD vasculitis and that Myl9 levels may be a useful biomarker to estimate inflammation and IVIG responsiveness to KD.
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spelling pubmed-98539062023-01-21 Increased Myosin light chain 9 expression during Kawasaki disease vasculitis Kobayashi, Hironobu Kimura, Motoko Y. Hasegawa, Ichita Suganuma, Eisuke Ikehara, Yuzuru Azuma, Kazuhiko Ito, Toshihiro Ebata, Ryota Kurashima, Yosuke Kawasaki, Yohei Shiko, Yuki Saito, Naoki Iwase, Hirotaro Lee, Youngho Noval Rivas, Magali Arditi, Moshe Zuka, Masahiko Hamada, Hiromichi Nakayama, Toshinori Front Immunol Immunology INTRODUCTION: Kawasaki disease (KD) is an acute systemic vasculitis that predominantly afflicts children. KD development is known to be associated with an aberrant immune response and abnormal platelet activation, however its etiology is still largely unknown. Myosin light chain 9 (Myl9) is known to regulate cellular contractility of both non-muscle and smooth muscle cells, and can be released from platelets, whereas any relations of Myl9 expression to KD vasculitis have not been examined. METHODS: Plasma Myl9 concentrations in KD patients and children with febrile illness were measured and associated with KD clinical course and prognosis. Myl9 release from platelets in KD patients was also evaluated in vitro. Myl9 expression was determined in coronary arteries from Lactobacillus casei cell wall extract (LCWE)-injected mice that develop experimental KD vasculitis, as well as in cardiac tissues obtained at autopsy from KD patients. RESULTS AND DISCUSSION: Plasma Myl9 levels were significantly higher in KD patients during the acute phase compared with healthy controls or patients with other febrile illnesses, declined following IVIG therapy in IVIG-responders but not in non-responders. In vitro, platelets from KD patients released Myl9 independently of thrombin stimulation. In the LCWE-injected mice, Myl9 was detected in cardiac tissue at an early stage before inflammatory cell infiltration was observed. In tissues obtained at autopsy from KD patients, the highest Myl9 expression was observed in thrombi during the acute phase and in the intima and adventitia of coronary arteries during the chronic phase. Thus, our studies show that Myl9 expression is significantly increased during KD vasculitis and that Myl9 levels may be a useful biomarker to estimate inflammation and IVIG responsiveness to KD. Frontiers Media S.A. 2023-01-06 /pmc/articles/PMC9853906/ /pubmed/36685558 http://dx.doi.org/10.3389/fimmu.2022.1036672 Text en Copyright © 2023 Kobayashi, Kimura, Hasegawa, Suganuma, Ikehara, Azuma, Ito, Ebata, Kurashima, Kawasaki, Shiko, Saito, Iwase, Lee, Noval Rivas, Arditi, Zuka, Hamada and Nakayama https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Kobayashi, Hironobu
Kimura, Motoko Y.
Hasegawa, Ichita
Suganuma, Eisuke
Ikehara, Yuzuru
Azuma, Kazuhiko
Ito, Toshihiro
Ebata, Ryota
Kurashima, Yosuke
Kawasaki, Yohei
Shiko, Yuki
Saito, Naoki
Iwase, Hirotaro
Lee, Youngho
Noval Rivas, Magali
Arditi, Moshe
Zuka, Masahiko
Hamada, Hiromichi
Nakayama, Toshinori
Increased Myosin light chain 9 expression during Kawasaki disease vasculitis
title Increased Myosin light chain 9 expression during Kawasaki disease vasculitis
title_full Increased Myosin light chain 9 expression during Kawasaki disease vasculitis
title_fullStr Increased Myosin light chain 9 expression during Kawasaki disease vasculitis
title_full_unstemmed Increased Myosin light chain 9 expression during Kawasaki disease vasculitis
title_short Increased Myosin light chain 9 expression during Kawasaki disease vasculitis
title_sort increased myosin light chain 9 expression during kawasaki disease vasculitis
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9853906/
https://www.ncbi.nlm.nih.gov/pubmed/36685558
http://dx.doi.org/10.3389/fimmu.2022.1036672
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