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CRISPR-dCas13-tracing reveals transcriptional memory and limited mRNA export in developing zebrafish embryos

BACKGROUND: Understanding gene transcription and mRNA-protein (mRNP) dynamics in single cells in a multicellular organism has been challenging. The catalytically dead CRISPR-Cas13 (dCas13) system has been used to visualize RNAs in live cells without genetic manipulation. We optimize this system to t...

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Detalles Bibliográficos
Autores principales:	Huang, Youkui, Gao, Bao-Qing, Meng, Quan, Yang, Liang-Zhong, Ma, Xu-Kai, Wu, Hao, Pan, Yu-Hang, Yang, Li, Li, Dong, Chen, Ling-Ling
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2023
Materias:	Research
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9854193/ https://www.ncbi.nlm.nih.gov/pubmed/36658633 http://dx.doi.org/10.1186/s13059-023-02848-6

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