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Intermediate-Term Storage of Spotted Halibut (Verasper variegatus) Sperm: Effects of Storage Methods, Extenders Supplemented with Antibiotics and Antioxidants on Sperm Quality

Intermediate-term preservation of sperm assists the reproductive management of fish spermatozoa; however, no information is available on sperm of the spotted halibut, Verasper variegatus. We aimed to identify the optimum diluents, temperatures, dilution ratios, antibiotics, and antioxidants for sper...

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Autores principales: Zidni, Irfan, Lee, Hyo-Bin, Yoon, Ji-Hye, Park, Jung-Yeol, Jang, Hyun-Seok, Co, Youn-Su, Pratiwi, Dian Yuni, Lim, Han-Kyu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9854475/
https://www.ncbi.nlm.nih.gov/pubmed/36670984
http://dx.doi.org/10.3390/antiox12010122
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author Zidni, Irfan
Lee, Hyo-Bin
Yoon, Ji-Hye
Park, Jung-Yeol
Jang, Hyun-Seok
Co, Youn-Su
Pratiwi, Dian Yuni
Lim, Han-Kyu
author_facet Zidni, Irfan
Lee, Hyo-Bin
Yoon, Ji-Hye
Park, Jung-Yeol
Jang, Hyun-Seok
Co, Youn-Su
Pratiwi, Dian Yuni
Lim, Han-Kyu
author_sort Zidni, Irfan
collection PubMed
description Intermediate-term preservation of sperm assists the reproductive management of fish spermatozoa; however, no information is available on sperm of the spotted halibut, Verasper variegatus. We aimed to identify the optimum diluents, temperatures, dilution ratios, antibiotics, and antioxidants for sperm motility and cell viability. The diluents evaluated were marine fish Ringer’s solution (MFRS), Stein’s solution, 300 mM sucrose, and 300 mM glucose (diluted 1:1 [sperm: diluent], 1:2, 1:4, and 1:10 and stored at 0, 2, 4, and 6 °C). Neomycin and gentamycin (100, 200, 400, and 800 mg/L) and antioxidants (Mito-TEMPO [0, 25, 50, 75, 100, 125, 150, 175, and 200 µM], reduced glutathione [0, 2, 4, 6, 8, and 10 mM], and trehalose [0, 50, 100, 150, 200, and 250 mM]) were assessed in terms of sperm preservation. The most effective condition for cold storage of spotted halibut sperm was Stein’s solution at a dilution ratio of 1:4 at 2 °C, with a combination of neomycin 800 mg/L and 250 mM trehalose that showed spermatozoa motility of > 43% after 60 days. These storage conditions will be valuable for spotted halibut hatcheries.
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spelling pubmed-98544752023-01-21 Intermediate-Term Storage of Spotted Halibut (Verasper variegatus) Sperm: Effects of Storage Methods, Extenders Supplemented with Antibiotics and Antioxidants on Sperm Quality Zidni, Irfan Lee, Hyo-Bin Yoon, Ji-Hye Park, Jung-Yeol Jang, Hyun-Seok Co, Youn-Su Pratiwi, Dian Yuni Lim, Han-Kyu Antioxidants (Basel) Article Intermediate-term preservation of sperm assists the reproductive management of fish spermatozoa; however, no information is available on sperm of the spotted halibut, Verasper variegatus. We aimed to identify the optimum diluents, temperatures, dilution ratios, antibiotics, and antioxidants for sperm motility and cell viability. The diluents evaluated were marine fish Ringer’s solution (MFRS), Stein’s solution, 300 mM sucrose, and 300 mM glucose (diluted 1:1 [sperm: diluent], 1:2, 1:4, and 1:10 and stored at 0, 2, 4, and 6 °C). Neomycin and gentamycin (100, 200, 400, and 800 mg/L) and antioxidants (Mito-TEMPO [0, 25, 50, 75, 100, 125, 150, 175, and 200 µM], reduced glutathione [0, 2, 4, 6, 8, and 10 mM], and trehalose [0, 50, 100, 150, 200, and 250 mM]) were assessed in terms of sperm preservation. The most effective condition for cold storage of spotted halibut sperm was Stein’s solution at a dilution ratio of 1:4 at 2 °C, with a combination of neomycin 800 mg/L and 250 mM trehalose that showed spermatozoa motility of > 43% after 60 days. These storage conditions will be valuable for spotted halibut hatcheries. MDPI 2023-01-03 /pmc/articles/PMC9854475/ /pubmed/36670984 http://dx.doi.org/10.3390/antiox12010122 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zidni, Irfan
Lee, Hyo-Bin
Yoon, Ji-Hye
Park, Jung-Yeol
Jang, Hyun-Seok
Co, Youn-Su
Pratiwi, Dian Yuni
Lim, Han-Kyu
Intermediate-Term Storage of Spotted Halibut (Verasper variegatus) Sperm: Effects of Storage Methods, Extenders Supplemented with Antibiotics and Antioxidants on Sperm Quality
title Intermediate-Term Storage of Spotted Halibut (Verasper variegatus) Sperm: Effects of Storage Methods, Extenders Supplemented with Antibiotics and Antioxidants on Sperm Quality
title_full Intermediate-Term Storage of Spotted Halibut (Verasper variegatus) Sperm: Effects of Storage Methods, Extenders Supplemented with Antibiotics and Antioxidants on Sperm Quality
title_fullStr Intermediate-Term Storage of Spotted Halibut (Verasper variegatus) Sperm: Effects of Storage Methods, Extenders Supplemented with Antibiotics and Antioxidants on Sperm Quality
title_full_unstemmed Intermediate-Term Storage of Spotted Halibut (Verasper variegatus) Sperm: Effects of Storage Methods, Extenders Supplemented with Antibiotics and Antioxidants on Sperm Quality
title_short Intermediate-Term Storage of Spotted Halibut (Verasper variegatus) Sperm: Effects of Storage Methods, Extenders Supplemented with Antibiotics and Antioxidants on Sperm Quality
title_sort intermediate-term storage of spotted halibut (verasper variegatus) sperm: effects of storage methods, extenders supplemented with antibiotics and antioxidants on sperm quality
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9854475/
https://www.ncbi.nlm.nih.gov/pubmed/36670984
http://dx.doi.org/10.3390/antiox12010122
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