Cargando…

Above the Invasive and Ornamental Attributes of the Traveler’s Palm: An In Vitro and In Silico Insight into the Anti-Oxidant, Anti-Enzymatic, Cytotoxic and Phytochemical Characterization of Ravenala madagascariensis

Ravenala madagascariensis is a widely known ornamental and medicinal plant, but with a dearth of scientific investigations regarding its phytochemical and pharmacological properties. Hence, these properties were appraised in this study. The DPPH (154.08 ± 2.43 mgTE/g), FRAP (249.40 ± 3.01 mgTE/g), C...

Descripción completa

Detalles Bibliográficos
Autores principales: Suroowan, Shanoo, Llorent-Martínez, Eulogio Jose, Zengin, Gokhan, Dall’Acqua, Stefano, Sut, Stefania, Buskaran, Kalaivani, Fakurazi, Sharida, Le Van, Bao, Abdalla, Mohnad, Abdalla, Ashraf N., Khalid, Asaad, Mahomoodally, Mohamad Fawzi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9854482/
https://www.ncbi.nlm.nih.gov/pubmed/36671049
http://dx.doi.org/10.3390/antiox12010184
_version_ 1784873131150671872
author Suroowan, Shanoo
Llorent-Martínez, Eulogio Jose
Zengin, Gokhan
Dall’Acqua, Stefano
Sut, Stefania
Buskaran, Kalaivani
Fakurazi, Sharida
Le Van, Bao
Abdalla, Mohnad
Abdalla, Ashraf N.
Khalid, Asaad
Mahomoodally, Mohamad Fawzi
author_facet Suroowan, Shanoo
Llorent-Martínez, Eulogio Jose
Zengin, Gokhan
Dall’Acqua, Stefano
Sut, Stefania
Buskaran, Kalaivani
Fakurazi, Sharida
Le Van, Bao
Abdalla, Mohnad
Abdalla, Ashraf N.
Khalid, Asaad
Mahomoodally, Mohamad Fawzi
author_sort Suroowan, Shanoo
collection PubMed
description Ravenala madagascariensis is a widely known ornamental and medicinal plant, but with a dearth of scientific investigations regarding its phytochemical and pharmacological properties. Hence, these properties were appraised in this study. The DPPH (154.08 ± 2.43 mgTE/g), FRAP (249.40 ± 3.01 mgTE/g), CUPRAC (384.57 ± 1.99 mgTE/g), metal chelating (29.68 ± 0.74 mgEDTAE/g) and phosphomolybdenum assay (2.38 ± 0.07 mmolTE/g) results demonstrated that the aqueous extract had the most prominent antioxidant activity, while the methanolic extract displayed the best antioxidant potential in the ABTS assay (438.46 ± 1.69 mgTE/g). The HPLC-ESI-Q-TOF-MS-MS analysis allowed the characterization of 41 metabolites. The methanolic extract was the most active against acetylcholinesterase. All extracts were active against the alpha-amylase and alpha-glucosidase enzymes, with the ethyl acetate extract being the most active against the alpha-amylase enzyme, while the methanolic extract showed the best alpha-glucosidase inhibition. A plethora of metabolites bonded more energetically with the assayed enzymes active sites based on the results of the in silico studies. R. madagascariensis extracts used in this study exhibited cytotoxicity against HT29 cells. The IC(50) of the methanolic extract was lower (506.99 ug/mL). Based on the heat map, whereby flavonoids were found to be in greater proportion in the extracts, it can be concluded that the flavonoid portion of the extracts contributed to the most activity.
format Online
Article
Text
id pubmed-9854482
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-98544822023-01-21 Above the Invasive and Ornamental Attributes of the Traveler’s Palm: An In Vitro and In Silico Insight into the Anti-Oxidant, Anti-Enzymatic, Cytotoxic and Phytochemical Characterization of Ravenala madagascariensis Suroowan, Shanoo Llorent-Martínez, Eulogio Jose Zengin, Gokhan Dall’Acqua, Stefano Sut, Stefania Buskaran, Kalaivani Fakurazi, Sharida Le Van, Bao Abdalla, Mohnad Abdalla, Ashraf N. Khalid, Asaad Mahomoodally, Mohamad Fawzi Antioxidants (Basel) Article Ravenala madagascariensis is a widely known ornamental and medicinal plant, but with a dearth of scientific investigations regarding its phytochemical and pharmacological properties. Hence, these properties were appraised in this study. The DPPH (154.08 ± 2.43 mgTE/g), FRAP (249.40 ± 3.01 mgTE/g), CUPRAC (384.57 ± 1.99 mgTE/g), metal chelating (29.68 ± 0.74 mgEDTAE/g) and phosphomolybdenum assay (2.38 ± 0.07 mmolTE/g) results demonstrated that the aqueous extract had the most prominent antioxidant activity, while the methanolic extract displayed the best antioxidant potential in the ABTS assay (438.46 ± 1.69 mgTE/g). The HPLC-ESI-Q-TOF-MS-MS analysis allowed the characterization of 41 metabolites. The methanolic extract was the most active against acetylcholinesterase. All extracts were active against the alpha-amylase and alpha-glucosidase enzymes, with the ethyl acetate extract being the most active against the alpha-amylase enzyme, while the methanolic extract showed the best alpha-glucosidase inhibition. A plethora of metabolites bonded more energetically with the assayed enzymes active sites based on the results of the in silico studies. R. madagascariensis extracts used in this study exhibited cytotoxicity against HT29 cells. The IC(50) of the methanolic extract was lower (506.99 ug/mL). Based on the heat map, whereby flavonoids were found to be in greater proportion in the extracts, it can be concluded that the flavonoid portion of the extracts contributed to the most activity. MDPI 2023-01-12 /pmc/articles/PMC9854482/ /pubmed/36671049 http://dx.doi.org/10.3390/antiox12010184 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Suroowan, Shanoo
Llorent-Martínez, Eulogio Jose
Zengin, Gokhan
Dall’Acqua, Stefano
Sut, Stefania
Buskaran, Kalaivani
Fakurazi, Sharida
Le Van, Bao
Abdalla, Mohnad
Abdalla, Ashraf N.
Khalid, Asaad
Mahomoodally, Mohamad Fawzi
Above the Invasive and Ornamental Attributes of the Traveler’s Palm: An In Vitro and In Silico Insight into the Anti-Oxidant, Anti-Enzymatic, Cytotoxic and Phytochemical Characterization of Ravenala madagascariensis
title Above the Invasive and Ornamental Attributes of the Traveler’s Palm: An In Vitro and In Silico Insight into the Anti-Oxidant, Anti-Enzymatic, Cytotoxic and Phytochemical Characterization of Ravenala madagascariensis
title_full Above the Invasive and Ornamental Attributes of the Traveler’s Palm: An In Vitro and In Silico Insight into the Anti-Oxidant, Anti-Enzymatic, Cytotoxic and Phytochemical Characterization of Ravenala madagascariensis
title_fullStr Above the Invasive and Ornamental Attributes of the Traveler’s Palm: An In Vitro and In Silico Insight into the Anti-Oxidant, Anti-Enzymatic, Cytotoxic and Phytochemical Characterization of Ravenala madagascariensis
title_full_unstemmed Above the Invasive and Ornamental Attributes of the Traveler’s Palm: An In Vitro and In Silico Insight into the Anti-Oxidant, Anti-Enzymatic, Cytotoxic and Phytochemical Characterization of Ravenala madagascariensis
title_short Above the Invasive and Ornamental Attributes of the Traveler’s Palm: An In Vitro and In Silico Insight into the Anti-Oxidant, Anti-Enzymatic, Cytotoxic and Phytochemical Characterization of Ravenala madagascariensis
title_sort above the invasive and ornamental attributes of the traveler’s palm: an in vitro and in silico insight into the anti-oxidant, anti-enzymatic, cytotoxic and phytochemical characterization of ravenala madagascariensis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9854482/
https://www.ncbi.nlm.nih.gov/pubmed/36671049
http://dx.doi.org/10.3390/antiox12010184
work_keys_str_mv AT suroowanshanoo abovetheinvasiveandornamentalattributesofthetravelerspalmaninvitroandinsilicoinsightintotheantioxidantantienzymaticcytotoxicandphytochemicalcharacterizationofravenalamadagascariensis
AT llorentmartinezeulogiojose abovetheinvasiveandornamentalattributesofthetravelerspalmaninvitroandinsilicoinsightintotheantioxidantantienzymaticcytotoxicandphytochemicalcharacterizationofravenalamadagascariensis
AT zengingokhan abovetheinvasiveandornamentalattributesofthetravelerspalmaninvitroandinsilicoinsightintotheantioxidantantienzymaticcytotoxicandphytochemicalcharacterizationofravenalamadagascariensis
AT dallacquastefano abovetheinvasiveandornamentalattributesofthetravelerspalmaninvitroandinsilicoinsightintotheantioxidantantienzymaticcytotoxicandphytochemicalcharacterizationofravenalamadagascariensis
AT sutstefania abovetheinvasiveandornamentalattributesofthetravelerspalmaninvitroandinsilicoinsightintotheantioxidantantienzymaticcytotoxicandphytochemicalcharacterizationofravenalamadagascariensis
AT buskarankalaivani abovetheinvasiveandornamentalattributesofthetravelerspalmaninvitroandinsilicoinsightintotheantioxidantantienzymaticcytotoxicandphytochemicalcharacterizationofravenalamadagascariensis
AT fakurazisharida abovetheinvasiveandornamentalattributesofthetravelerspalmaninvitroandinsilicoinsightintotheantioxidantantienzymaticcytotoxicandphytochemicalcharacterizationofravenalamadagascariensis
AT levanbao abovetheinvasiveandornamentalattributesofthetravelerspalmaninvitroandinsilicoinsightintotheantioxidantantienzymaticcytotoxicandphytochemicalcharacterizationofravenalamadagascariensis
AT abdallamohnad abovetheinvasiveandornamentalattributesofthetravelerspalmaninvitroandinsilicoinsightintotheantioxidantantienzymaticcytotoxicandphytochemicalcharacterizationofravenalamadagascariensis
AT abdallaashrafn abovetheinvasiveandornamentalattributesofthetravelerspalmaninvitroandinsilicoinsightintotheantioxidantantienzymaticcytotoxicandphytochemicalcharacterizationofravenalamadagascariensis
AT khalidasaad abovetheinvasiveandornamentalattributesofthetravelerspalmaninvitroandinsilicoinsightintotheantioxidantantienzymaticcytotoxicandphytochemicalcharacterizationofravenalamadagascariensis
AT mahomoodallymohamadfawzi abovetheinvasiveandornamentalattributesofthetravelerspalmaninvitroandinsilicoinsightintotheantioxidantantienzymaticcytotoxicandphytochemicalcharacterizationofravenalamadagascariensis