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A Two-Compartment Fermentation System to Quantify Strain-Specific Interactions in Microbial Co-Cultures

To fulfil the growing interest in investigating microbial interactions in co-cultures, a novel two-compartment bioreactor system was developed, characterised, and implemented. The system allowed for the exchange of amino acids and peptides via a polyethersulfone membrane that retained biomass. Furth...

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Autores principales: Ulmer, Andreas, Veit, Stefan, Erdemann, Florian, Freund, Andreas, Loesch, Maren, Teleki, Attila, Zeidan, Ahmad A., Takors, Ralf
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9854596/
https://www.ncbi.nlm.nih.gov/pubmed/36671675
http://dx.doi.org/10.3390/bioengineering10010103
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author Ulmer, Andreas
Veit, Stefan
Erdemann, Florian
Freund, Andreas
Loesch, Maren
Teleki, Attila
Zeidan, Ahmad A.
Takors, Ralf
author_facet Ulmer, Andreas
Veit, Stefan
Erdemann, Florian
Freund, Andreas
Loesch, Maren
Teleki, Attila
Zeidan, Ahmad A.
Takors, Ralf
author_sort Ulmer, Andreas
collection PubMed
description To fulfil the growing interest in investigating microbial interactions in co-cultures, a novel two-compartment bioreactor system was developed, characterised, and implemented. The system allowed for the exchange of amino acids and peptides via a polyethersulfone membrane that retained biomass. Further system characterisation revealed a Bodenstein number of 18, which hints at backmixing. Together with other physical settings, the existence of unwanted inner-compartment substrate gradients could be ruled out. Furthermore, the study of Damkoehler numbers indicated that a proper metabolite supply between compartments was enabled. Implementing the two-compartment system (2cs) for growing Streptococcus thermophilus and Lactobacillus delbrueckii subs. bulgaricus, which are microorganisms commonly used in yogurt starter cultures, revealed only a small variance between the one-compartment and two-compartment approaches. The 2cs enabled the quantification of the strain-specific production and consumption rates of amino acids in an interacting S. thermophilus–L. bulgaricus co-culture. Therefore, comparisons between mono- and co-culture performance could be achieved. Both species produce and release amino acids. Only alanine was produced de novo from glucose through potential transaminase activity by L. bulgaricus and consumed by S. thermophilus. Arginine availability in peptides was limited to S. thermophilus’ growth, indicating active biosynthesis and dependency on the proteolytic activity of L. bulgaricus. The application of the 2cs not only opens the door for the quantification of exchange fluxes between microbes but also enables continuous production modes, for example, for targeted evolution studies.
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spelling pubmed-98545962023-01-21 A Two-Compartment Fermentation System to Quantify Strain-Specific Interactions in Microbial Co-Cultures Ulmer, Andreas Veit, Stefan Erdemann, Florian Freund, Andreas Loesch, Maren Teleki, Attila Zeidan, Ahmad A. Takors, Ralf Bioengineering (Basel) Article To fulfil the growing interest in investigating microbial interactions in co-cultures, a novel two-compartment bioreactor system was developed, characterised, and implemented. The system allowed for the exchange of amino acids and peptides via a polyethersulfone membrane that retained biomass. Further system characterisation revealed a Bodenstein number of 18, which hints at backmixing. Together with other physical settings, the existence of unwanted inner-compartment substrate gradients could be ruled out. Furthermore, the study of Damkoehler numbers indicated that a proper metabolite supply between compartments was enabled. Implementing the two-compartment system (2cs) for growing Streptococcus thermophilus and Lactobacillus delbrueckii subs. bulgaricus, which are microorganisms commonly used in yogurt starter cultures, revealed only a small variance between the one-compartment and two-compartment approaches. The 2cs enabled the quantification of the strain-specific production and consumption rates of amino acids in an interacting S. thermophilus–L. bulgaricus co-culture. Therefore, comparisons between mono- and co-culture performance could be achieved. Both species produce and release amino acids. Only alanine was produced de novo from glucose through potential transaminase activity by L. bulgaricus and consumed by S. thermophilus. Arginine availability in peptides was limited to S. thermophilus’ growth, indicating active biosynthesis and dependency on the proteolytic activity of L. bulgaricus. The application of the 2cs not only opens the door for the quantification of exchange fluxes between microbes but also enables continuous production modes, for example, for targeted evolution studies. MDPI 2023-01-11 /pmc/articles/PMC9854596/ /pubmed/36671675 http://dx.doi.org/10.3390/bioengineering10010103 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ulmer, Andreas
Veit, Stefan
Erdemann, Florian
Freund, Andreas
Loesch, Maren
Teleki, Attila
Zeidan, Ahmad A.
Takors, Ralf
A Two-Compartment Fermentation System to Quantify Strain-Specific Interactions in Microbial Co-Cultures
title A Two-Compartment Fermentation System to Quantify Strain-Specific Interactions in Microbial Co-Cultures
title_full A Two-Compartment Fermentation System to Quantify Strain-Specific Interactions in Microbial Co-Cultures
title_fullStr A Two-Compartment Fermentation System to Quantify Strain-Specific Interactions in Microbial Co-Cultures
title_full_unstemmed A Two-Compartment Fermentation System to Quantify Strain-Specific Interactions in Microbial Co-Cultures
title_short A Two-Compartment Fermentation System to Quantify Strain-Specific Interactions in Microbial Co-Cultures
title_sort two-compartment fermentation system to quantify strain-specific interactions in microbial co-cultures
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9854596/
https://www.ncbi.nlm.nih.gov/pubmed/36671675
http://dx.doi.org/10.3390/bioengineering10010103
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