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Disease–Gene Networks of Skin Pigmentation Disorders and Reconstruction of Protein–Protein Interaction Networks

Melanin, a light and free radical absorbing pigment, is produced in melanocyte cells that are found in skin, but also in hair follicles, eyes, the inner ear, heart, brain and other organs. Melanin synthesis is the result of a complex network of signaling and metabolic reactions. It therefore comes a...

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Autores principales: D’Arcy, Cian, Bass, Olivia, Junk, Philipp, Sevrin, Thomas, Oliviero, Giorgio, Wynne, Kieran, Halasz, Melinda, Kiel, Christina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9854651/
https://www.ncbi.nlm.nih.gov/pubmed/36671585
http://dx.doi.org/10.3390/bioengineering10010013
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author D’Arcy, Cian
Bass, Olivia
Junk, Philipp
Sevrin, Thomas
Oliviero, Giorgio
Wynne, Kieran
Halasz, Melinda
Kiel, Christina
author_facet D’Arcy, Cian
Bass, Olivia
Junk, Philipp
Sevrin, Thomas
Oliviero, Giorgio
Wynne, Kieran
Halasz, Melinda
Kiel, Christina
author_sort D’Arcy, Cian
collection PubMed
description Melanin, a light and free radical absorbing pigment, is produced in melanocyte cells that are found in skin, but also in hair follicles, eyes, the inner ear, heart, brain and other organs. Melanin synthesis is the result of a complex network of signaling and metabolic reactions. It therefore comes as no surprise that mutations in many of the genes involved are associated with various types of pigmentation diseases and phenotypes (‘pigmentation genes’). Here, we used bioinformatics tools to first reconstruct gene-disease/phenotype associations for all pigmentation genes. Next, we reconstructed protein–protein interaction (PPI) networks centered around pigmentation gene products (‘pigmentation proteins’) and supplemented the PPI networks with protein expression information obtained by mass spectrometry in a panel of melanoma cell lines (both pigment producing and non-pigment producing cells). The analysis provides a systems network representation of all genes/ proteins centered around pigmentation and melanin biosynthesis pathways (‘pigmentation network map’). Our work will enable the pigmentation research community to experimentally test new hypothesis arising from the pigmentation network map and to identify new targets for drug discovery.
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spelling pubmed-98546512023-01-21 Disease–Gene Networks of Skin Pigmentation Disorders and Reconstruction of Protein–Protein Interaction Networks D’Arcy, Cian Bass, Olivia Junk, Philipp Sevrin, Thomas Oliviero, Giorgio Wynne, Kieran Halasz, Melinda Kiel, Christina Bioengineering (Basel) Article Melanin, a light and free radical absorbing pigment, is produced in melanocyte cells that are found in skin, but also in hair follicles, eyes, the inner ear, heart, brain and other organs. Melanin synthesis is the result of a complex network of signaling and metabolic reactions. It therefore comes as no surprise that mutations in many of the genes involved are associated with various types of pigmentation diseases and phenotypes (‘pigmentation genes’). Here, we used bioinformatics tools to first reconstruct gene-disease/phenotype associations for all pigmentation genes. Next, we reconstructed protein–protein interaction (PPI) networks centered around pigmentation gene products (‘pigmentation proteins’) and supplemented the PPI networks with protein expression information obtained by mass spectrometry in a panel of melanoma cell lines (both pigment producing and non-pigment producing cells). The analysis provides a systems network representation of all genes/ proteins centered around pigmentation and melanin biosynthesis pathways (‘pigmentation network map’). Our work will enable the pigmentation research community to experimentally test new hypothesis arising from the pigmentation network map and to identify new targets for drug discovery. MDPI 2022-12-21 /pmc/articles/PMC9854651/ /pubmed/36671585 http://dx.doi.org/10.3390/bioengineering10010013 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
D’Arcy, Cian
Bass, Olivia
Junk, Philipp
Sevrin, Thomas
Oliviero, Giorgio
Wynne, Kieran
Halasz, Melinda
Kiel, Christina
Disease–Gene Networks of Skin Pigmentation Disorders and Reconstruction of Protein–Protein Interaction Networks
title Disease–Gene Networks of Skin Pigmentation Disorders and Reconstruction of Protein–Protein Interaction Networks
title_full Disease–Gene Networks of Skin Pigmentation Disorders and Reconstruction of Protein–Protein Interaction Networks
title_fullStr Disease–Gene Networks of Skin Pigmentation Disorders and Reconstruction of Protein–Protein Interaction Networks
title_full_unstemmed Disease–Gene Networks of Skin Pigmentation Disorders and Reconstruction of Protein–Protein Interaction Networks
title_short Disease–Gene Networks of Skin Pigmentation Disorders and Reconstruction of Protein–Protein Interaction Networks
title_sort disease–gene networks of skin pigmentation disorders and reconstruction of protein–protein interaction networks
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9854651/
https://www.ncbi.nlm.nih.gov/pubmed/36671585
http://dx.doi.org/10.3390/bioengineering10010013
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