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Multidrug-Resistant ESBL-Producing E. coli in Clinical Samples from the UK

Globally, cephalosporin therapy failure is a serious problem for infection control. One causative agent of cephalosporin-resistant infections is multidrug-resistant (MDR) E. coli producing extended-spectrum β-lactamases (ESBLs) and/or plasmid-encoded AmpC (pAmpC) β-lactamases. We evaluated the occur...

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Autores principales: Ibrahim, Delveen R., Dodd, Christine E. R., Stekel, Dov J., Meshioye, Remilekun T., Diggle, Mathew, Lister, Michelle, Hobman, Jon L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9854697/
https://www.ncbi.nlm.nih.gov/pubmed/36671370
http://dx.doi.org/10.3390/antibiotics12010169
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author Ibrahim, Delveen R.
Dodd, Christine E. R.
Stekel, Dov J.
Meshioye, Remilekun T.
Diggle, Mathew
Lister, Michelle
Hobman, Jon L.
author_facet Ibrahim, Delveen R.
Dodd, Christine E. R.
Stekel, Dov J.
Meshioye, Remilekun T.
Diggle, Mathew
Lister, Michelle
Hobman, Jon L.
author_sort Ibrahim, Delveen R.
collection PubMed
description Globally, cephalosporin therapy failure is a serious problem for infection control. One causative agent of cephalosporin-resistant infections is multidrug-resistant (MDR) E. coli producing extended-spectrum β-lactamases (ESBLs) and/or plasmid-encoded AmpC (pAmpC) β-lactamases. We evaluated the occurrence of ESBL/pAmpC genetic determinants in phenotypically MDR E. coli isolated from clinical samples of blood, faeces, ear effusion, urine and sputum from a UK hospital. Phenotypic resistance profiling for 18 antibiotics (from seven classes) showed that 32/35 isolates were MDR, with resistance to 4–16 of the tested antibiotics. Of the isolates, 97.1% showed resistance to ampicillin, 71.4% showed resistance to co-amoxiclav, cefotaxime, ceftazidime and ceftiofur, and 68.5% showed resistance to cefquinome. bla(CTX-M), bla(TEM) and bla(OXA-1) genes were detected in 23, 13 and 12 strains, respectively, and Intl1 was detected in 17 isolates. The most common subtypes among the definite sequence types were CTX-M-15 (40%) and TEM-1 (75%). No E. coli isolates carried pAmpC genes. Significant correlations were seen between CTX-M carriage and cefotaxime, ceftiofur, aztreonam, ceftazidime and cefquinome resistance; between bla(CTX-M), bla(TEM) and bla(OXA-1) carriage and ciprofloxacin resistance; and between Intl1 carriage and trimethoprim/sulfamethoxazole resistance. Thus, MDR phenotypes may be conferred by a relatively small number of genes. The level and pattern of antibiotic resistance highlight the need for better antibiotic therapy guidelines, including reduced use and improved surveillance.
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spelling pubmed-98546972023-01-21 Multidrug-Resistant ESBL-Producing E. coli in Clinical Samples from the UK Ibrahim, Delveen R. Dodd, Christine E. R. Stekel, Dov J. Meshioye, Remilekun T. Diggle, Mathew Lister, Michelle Hobman, Jon L. Antibiotics (Basel) Article Globally, cephalosporin therapy failure is a serious problem for infection control. One causative agent of cephalosporin-resistant infections is multidrug-resistant (MDR) E. coli producing extended-spectrum β-lactamases (ESBLs) and/or plasmid-encoded AmpC (pAmpC) β-lactamases. We evaluated the occurrence of ESBL/pAmpC genetic determinants in phenotypically MDR E. coli isolated from clinical samples of blood, faeces, ear effusion, urine and sputum from a UK hospital. Phenotypic resistance profiling for 18 antibiotics (from seven classes) showed that 32/35 isolates were MDR, with resistance to 4–16 of the tested antibiotics. Of the isolates, 97.1% showed resistance to ampicillin, 71.4% showed resistance to co-amoxiclav, cefotaxime, ceftazidime and ceftiofur, and 68.5% showed resistance to cefquinome. bla(CTX-M), bla(TEM) and bla(OXA-1) genes were detected in 23, 13 and 12 strains, respectively, and Intl1 was detected in 17 isolates. The most common subtypes among the definite sequence types were CTX-M-15 (40%) and TEM-1 (75%). No E. coli isolates carried pAmpC genes. Significant correlations were seen between CTX-M carriage and cefotaxime, ceftiofur, aztreonam, ceftazidime and cefquinome resistance; between bla(CTX-M), bla(TEM) and bla(OXA-1) carriage and ciprofloxacin resistance; and between Intl1 carriage and trimethoprim/sulfamethoxazole resistance. Thus, MDR phenotypes may be conferred by a relatively small number of genes. The level and pattern of antibiotic resistance highlight the need for better antibiotic therapy guidelines, including reduced use and improved surveillance. MDPI 2023-01-13 /pmc/articles/PMC9854697/ /pubmed/36671370 http://dx.doi.org/10.3390/antibiotics12010169 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ibrahim, Delveen R.
Dodd, Christine E. R.
Stekel, Dov J.
Meshioye, Remilekun T.
Diggle, Mathew
Lister, Michelle
Hobman, Jon L.
Multidrug-Resistant ESBL-Producing E. coli in Clinical Samples from the UK
title Multidrug-Resistant ESBL-Producing E. coli in Clinical Samples from the UK
title_full Multidrug-Resistant ESBL-Producing E. coli in Clinical Samples from the UK
title_fullStr Multidrug-Resistant ESBL-Producing E. coli in Clinical Samples from the UK
title_full_unstemmed Multidrug-Resistant ESBL-Producing E. coli in Clinical Samples from the UK
title_short Multidrug-Resistant ESBL-Producing E. coli in Clinical Samples from the UK
title_sort multidrug-resistant esbl-producing e. coli in clinical samples from the uk
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9854697/
https://www.ncbi.nlm.nih.gov/pubmed/36671370
http://dx.doi.org/10.3390/antibiotics12010169
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