Cargando…

Characteristics of tet(X4)−Producing Escherichia coli in Chicken and Pig Farms in Hunan Province, China

Background: The plasmid−mediated tigecycline resistance gene tet(X4) confers a high level of resistance to tigecycline. The experiment aims to investigate the prevalence and characterization of tet(X4) in Escherichia coli isolates from chicken and pig farms in Hunan province, China. Methods: A total...

Descripción completa

Detalles Bibliográficos
Autores principales: Yang, Jie, Xiao, Gang, Xiao, Ning, Jiang, Zonghan, Jiang, Chao, Li, Yujuan, Chen, Wenxin, Lin, Hongguang, Sun, Zhiliang, Li, Jiyun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9854778/
https://www.ncbi.nlm.nih.gov/pubmed/36671348
http://dx.doi.org/10.3390/antibiotics12010147
_version_ 1784873207191306240
author Yang, Jie
Xiao, Gang
Xiao, Ning
Jiang, Zonghan
Jiang, Chao
Li, Yujuan
Chen, Wenxin
Lin, Hongguang
Sun, Zhiliang
Li, Jiyun
author_facet Yang, Jie
Xiao, Gang
Xiao, Ning
Jiang, Zonghan
Jiang, Chao
Li, Yujuan
Chen, Wenxin
Lin, Hongguang
Sun, Zhiliang
Li, Jiyun
author_sort Yang, Jie
collection PubMed
description Background: The plasmid−mediated tigecycline resistance gene tet(X4) confers a high level of resistance to tigecycline. The experiment aims to investigate the prevalence and characterization of tet(X4) in Escherichia coli isolates from chicken and pig farms in Hunan province, China. Methods: A total of six tet(X4) positive strains were identified in 257 E. coli derived from chicken samples in Xiangtan city (n = 2), pig samples in Xiangxiang city (n = 1), Chenzhou city (n = 2), and Zhuzhou city (n = 1). The presence of tet(X4) was directly detected by PCR assay, and then the broth dilution method determined the antimicrobial susceptibility profile of the tet(X4)−positive isolates. Genomic locations were identified by whole−genome sequencing (WGS) and bioinformatics. Results: Almost all tet(X4)−positive strains showed high resistance to multidrug, including tigecycline. Resistome analysis revealed many antibiotic resistance genes, including those with resistance to tetracyclines, β−lactams, phenicols, quinolones, lincosamides chloramphenicol, aminoglycosides and sulfamids. These tet(X4)−bearing strains exhibited six distract STs, such as ST10, 202, ST218, ST362, ST2077, ST7068. The plasmid replicon types carrying tet(X4) were the hybrid plasmid IncFIA(HI1)/IncHIA/IncHIB(R27) (5/6) and IncX1 (1/6). Conclusions: The presence of similar genetic environments in E. coli from different cities suggests there may be horizontal transmission pathways promoting the broad spread of drug−resistant genes in Hunan Province, putting great pressure on multidrug resistance monitoring.
format Online
Article
Text
id pubmed-9854778
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-98547782023-01-21 Characteristics of tet(X4)−Producing Escherichia coli in Chicken and Pig Farms in Hunan Province, China Yang, Jie Xiao, Gang Xiao, Ning Jiang, Zonghan Jiang, Chao Li, Yujuan Chen, Wenxin Lin, Hongguang Sun, Zhiliang Li, Jiyun Antibiotics (Basel) Article Background: The plasmid−mediated tigecycline resistance gene tet(X4) confers a high level of resistance to tigecycline. The experiment aims to investigate the prevalence and characterization of tet(X4) in Escherichia coli isolates from chicken and pig farms in Hunan province, China. Methods: A total of six tet(X4) positive strains were identified in 257 E. coli derived from chicken samples in Xiangtan city (n = 2), pig samples in Xiangxiang city (n = 1), Chenzhou city (n = 2), and Zhuzhou city (n = 1). The presence of tet(X4) was directly detected by PCR assay, and then the broth dilution method determined the antimicrobial susceptibility profile of the tet(X4)−positive isolates. Genomic locations were identified by whole−genome sequencing (WGS) and bioinformatics. Results: Almost all tet(X4)−positive strains showed high resistance to multidrug, including tigecycline. Resistome analysis revealed many antibiotic resistance genes, including those with resistance to tetracyclines, β−lactams, phenicols, quinolones, lincosamides chloramphenicol, aminoglycosides and sulfamids. These tet(X4)−bearing strains exhibited six distract STs, such as ST10, 202, ST218, ST362, ST2077, ST7068. The plasmid replicon types carrying tet(X4) were the hybrid plasmid IncFIA(HI1)/IncHIA/IncHIB(R27) (5/6) and IncX1 (1/6). Conclusions: The presence of similar genetic environments in E. coli from different cities suggests there may be horizontal transmission pathways promoting the broad spread of drug−resistant genes in Hunan Province, putting great pressure on multidrug resistance monitoring. MDPI 2023-01-11 /pmc/articles/PMC9854778/ /pubmed/36671348 http://dx.doi.org/10.3390/antibiotics12010147 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yang, Jie
Xiao, Gang
Xiao, Ning
Jiang, Zonghan
Jiang, Chao
Li, Yujuan
Chen, Wenxin
Lin, Hongguang
Sun, Zhiliang
Li, Jiyun
Characteristics of tet(X4)−Producing Escherichia coli in Chicken and Pig Farms in Hunan Province, China
title Characteristics of tet(X4)−Producing Escherichia coli in Chicken and Pig Farms in Hunan Province, China
title_full Characteristics of tet(X4)−Producing Escherichia coli in Chicken and Pig Farms in Hunan Province, China
title_fullStr Characteristics of tet(X4)−Producing Escherichia coli in Chicken and Pig Farms in Hunan Province, China
title_full_unstemmed Characteristics of tet(X4)−Producing Escherichia coli in Chicken and Pig Farms in Hunan Province, China
title_short Characteristics of tet(X4)−Producing Escherichia coli in Chicken and Pig Farms in Hunan Province, China
title_sort characteristics of tet(x4)−producing escherichia coli in chicken and pig farms in hunan province, china
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9854778/
https://www.ncbi.nlm.nih.gov/pubmed/36671348
http://dx.doi.org/10.3390/antibiotics12010147
work_keys_str_mv AT yangjie characteristicsoftetx4producingescherichiacoliinchickenandpigfarmsinhunanprovincechina
AT xiaogang characteristicsoftetx4producingescherichiacoliinchickenandpigfarmsinhunanprovincechina
AT xiaoning characteristicsoftetx4producingescherichiacoliinchickenandpigfarmsinhunanprovincechina
AT jiangzonghan characteristicsoftetx4producingescherichiacoliinchickenandpigfarmsinhunanprovincechina
AT jiangchao characteristicsoftetx4producingescherichiacoliinchickenandpigfarmsinhunanprovincechina
AT liyujuan characteristicsoftetx4producingescherichiacoliinchickenandpigfarmsinhunanprovincechina
AT chenwenxin characteristicsoftetx4producingescherichiacoliinchickenandpigfarmsinhunanprovincechina
AT linhongguang characteristicsoftetx4producingescherichiacoliinchickenandpigfarmsinhunanprovincechina
AT sunzhiliang characteristicsoftetx4producingescherichiacoliinchickenandpigfarmsinhunanprovincechina
AT lijiyun characteristicsoftetx4producingescherichiacoliinchickenandpigfarmsinhunanprovincechina