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Antimicrobial Susceptibility and Genetic Prevalence of Extended-Spectrum β-Lactamases in Gram-Negative Rods Isolated from Clinical Specimens in Pakistan
The prevalence of extended-spectrum β-lactamase (ESBL) genes has increased remarkably, resulting in multidrug-resistant gram-negative rods (GNRs) in clinical specimens. This cross-sectional study aimed to determine the antimicrobial susceptibility of ESBL-producing GNRs and its correlation with corr...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9854846/ https://www.ncbi.nlm.nih.gov/pubmed/36671229 http://dx.doi.org/10.3390/antibiotics12010029 |
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author | Idrees, Muhammad Mubashar Rimsha, Rimsha Idrees, Muhammad Daoud Saeed, Ali |
author_facet | Idrees, Muhammad Mubashar Rimsha, Rimsha Idrees, Muhammad Daoud Saeed, Ali |
author_sort | Idrees, Muhammad Mubashar |
collection | PubMed |
description | The prevalence of extended-spectrum β-lactamase (ESBL) genes has increased remarkably, resulting in multidrug-resistant gram-negative rods (GNRs) in clinical specimens. This cross-sectional study aimed to determine the antimicrobial susceptibility of ESBL-producing GNRs and its correlation with corresponding genes. Two hundred and seventy-two (n = 272) samples were evaluated for the molecular identification of ESBL genes by polymerase chain reaction after confirmation with the modified double-disc synergy test. E. coli 64.0% (n = 174) was the most prevalent ESBL producer, followed by Klebsiella species 27.2% (n = seventy-four), Acinetobacter species 6.6% (n = eighteen) and others 2.2% (n = six). These ESBL-producing isolates showed resistance to β-lactam antibiotics, i.e., sulbactam/cefoperazone (41.5%), piperacillin/tazobactam (39.3%), meropenem (36.0%), imipenem (34.2%) and non- β-lactam antibiotics, i.e., nalidixic acid (89.0%), co-trimoxazole (84.9%), ciprofloxacin (82.4%), gentamicin (46.3%), nitrofurantoin (24.6%), amikacin (19.9%) and fosfomycin (19.9%). The incidences of the ESBLs-producing genes bla(CTX-M,) bla(TEM), bla(OXA) and bla(SHV) were 91.2%, 61.8%, 39.3% and 17.6%, respectively. Among nine multiple-gene combinations, bla(CTX-M) + bla(TEM) (30.5%) was the most prevalent combination, followed by bla(CTX-M) + bla(OXA) + bla(TEM) (14.0%), bla(CTX-M) + bla(OXA) (13.6%), bla(CTX-M) + bla(TEM) + bla(SHV) (7.0%), bla(CTX-M) + bla(SHV) (2.2%), bla(CTX-M) + bla(OXA) + bla(SHV) (2.2%) and bla(OXA) + bla(TEM) (1.8%). ESBLs producing GNRs carrying bla(CTX-M,) bla(TEM), bla(OXA) and bla(SHV) showed resistances to β-lactam antibiotics, i.e., ampicillin, amoxillin-clavulanic acid, cefotaxime and ceftazidime but were susceptible to carbapenems (meropenem and imipenem), β-lactam-β-lactamase inhibitor combination (piperacillin/tazobactam) and non-β-lactam antibiotics i.e., aminoglycoside (amikacin and gentamicin), nitrofurantoin and fosfomycin. These antibiotics that demonstrated activity may be used to treat infections in clinical settings. |
format | Online Article Text |
id | pubmed-9854846 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-98548462023-01-21 Antimicrobial Susceptibility and Genetic Prevalence of Extended-Spectrum β-Lactamases in Gram-Negative Rods Isolated from Clinical Specimens in Pakistan Idrees, Muhammad Mubashar Rimsha, Rimsha Idrees, Muhammad Daoud Saeed, Ali Antibiotics (Basel) Article The prevalence of extended-spectrum β-lactamase (ESBL) genes has increased remarkably, resulting in multidrug-resistant gram-negative rods (GNRs) in clinical specimens. This cross-sectional study aimed to determine the antimicrobial susceptibility of ESBL-producing GNRs and its correlation with corresponding genes. Two hundred and seventy-two (n = 272) samples were evaluated for the molecular identification of ESBL genes by polymerase chain reaction after confirmation with the modified double-disc synergy test. E. coli 64.0% (n = 174) was the most prevalent ESBL producer, followed by Klebsiella species 27.2% (n = seventy-four), Acinetobacter species 6.6% (n = eighteen) and others 2.2% (n = six). These ESBL-producing isolates showed resistance to β-lactam antibiotics, i.e., sulbactam/cefoperazone (41.5%), piperacillin/tazobactam (39.3%), meropenem (36.0%), imipenem (34.2%) and non- β-lactam antibiotics, i.e., nalidixic acid (89.0%), co-trimoxazole (84.9%), ciprofloxacin (82.4%), gentamicin (46.3%), nitrofurantoin (24.6%), amikacin (19.9%) and fosfomycin (19.9%). The incidences of the ESBLs-producing genes bla(CTX-M,) bla(TEM), bla(OXA) and bla(SHV) were 91.2%, 61.8%, 39.3% and 17.6%, respectively. Among nine multiple-gene combinations, bla(CTX-M) + bla(TEM) (30.5%) was the most prevalent combination, followed by bla(CTX-M) + bla(OXA) + bla(TEM) (14.0%), bla(CTX-M) + bla(OXA) (13.6%), bla(CTX-M) + bla(TEM) + bla(SHV) (7.0%), bla(CTX-M) + bla(SHV) (2.2%), bla(CTX-M) + bla(OXA) + bla(SHV) (2.2%) and bla(OXA) + bla(TEM) (1.8%). ESBLs producing GNRs carrying bla(CTX-M,) bla(TEM), bla(OXA) and bla(SHV) showed resistances to β-lactam antibiotics, i.e., ampicillin, amoxillin-clavulanic acid, cefotaxime and ceftazidime but were susceptible to carbapenems (meropenem and imipenem), β-lactam-β-lactamase inhibitor combination (piperacillin/tazobactam) and non-β-lactam antibiotics i.e., aminoglycoside (amikacin and gentamicin), nitrofurantoin and fosfomycin. These antibiotics that demonstrated activity may be used to treat infections in clinical settings. MDPI 2022-12-24 /pmc/articles/PMC9854846/ /pubmed/36671229 http://dx.doi.org/10.3390/antibiotics12010029 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Idrees, Muhammad Mubashar Rimsha, Rimsha Idrees, Muhammad Daoud Saeed, Ali Antimicrobial Susceptibility and Genetic Prevalence of Extended-Spectrum β-Lactamases in Gram-Negative Rods Isolated from Clinical Specimens in Pakistan |
title | Antimicrobial Susceptibility and Genetic Prevalence of Extended-Spectrum β-Lactamases in Gram-Negative Rods Isolated from Clinical Specimens in Pakistan |
title_full | Antimicrobial Susceptibility and Genetic Prevalence of Extended-Spectrum β-Lactamases in Gram-Negative Rods Isolated from Clinical Specimens in Pakistan |
title_fullStr | Antimicrobial Susceptibility and Genetic Prevalence of Extended-Spectrum β-Lactamases in Gram-Negative Rods Isolated from Clinical Specimens in Pakistan |
title_full_unstemmed | Antimicrobial Susceptibility and Genetic Prevalence of Extended-Spectrum β-Lactamases in Gram-Negative Rods Isolated from Clinical Specimens in Pakistan |
title_short | Antimicrobial Susceptibility and Genetic Prevalence of Extended-Spectrum β-Lactamases in Gram-Negative Rods Isolated from Clinical Specimens in Pakistan |
title_sort | antimicrobial susceptibility and genetic prevalence of extended-spectrum β-lactamases in gram-negative rods isolated from clinical specimens in pakistan |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9854846/ https://www.ncbi.nlm.nih.gov/pubmed/36671229 http://dx.doi.org/10.3390/antibiotics12010029 |
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