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Anti-Osteoarthritic Effects of Prunella Vulgaris and Gentiana Lutea In Vitro and In Vivo

Osteoarthritis (OA) is the progressive destruction of articular cartilage with severe symptoms, including pain and stiffness. We investigated the anti-osteoarthritic effects of Prunella vulgaris (PV) and Gentiana lutea (GL) extract in primary cultured chondrocytes RAW 264.7 cells in vitro and destab...

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Autores principales: Kim, Jeonghyun, Lee, Chang-Gun, Hwang, Seokjin, Yun, Seung-Hee, Uprety, Laxmi Prasad, Oh, Kang-Il, Singh, Shivani, Yoo, Jisu, Jeong, Hyesoo, Yong, Yoonjoong, Yeo, Subin, Park, Eunkuk, Jeong, Seon-Yong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9854930/
https://www.ncbi.nlm.nih.gov/pubmed/36670908
http://dx.doi.org/10.3390/antiox12010047
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author Kim, Jeonghyun
Lee, Chang-Gun
Hwang, Seokjin
Yun, Seung-Hee
Uprety, Laxmi Prasad
Oh, Kang-Il
Singh, Shivani
Yoo, Jisu
Jeong, Hyesoo
Yong, Yoonjoong
Yeo, Subin
Park, Eunkuk
Jeong, Seon-Yong
author_facet Kim, Jeonghyun
Lee, Chang-Gun
Hwang, Seokjin
Yun, Seung-Hee
Uprety, Laxmi Prasad
Oh, Kang-Il
Singh, Shivani
Yoo, Jisu
Jeong, Hyesoo
Yong, Yoonjoong
Yeo, Subin
Park, Eunkuk
Jeong, Seon-Yong
author_sort Kim, Jeonghyun
collection PubMed
description Osteoarthritis (OA) is the progressive destruction of articular cartilage with severe symptoms, including pain and stiffness. We investigated the anti-osteoarthritic effects of Prunella vulgaris (PV) and Gentiana lutea (GL) extract in primary cultured chondrocytes RAW 264.7 cells in vitro and destabilization of the medial meniscus (DMM)-induced OA mice in vivo. Primary chondrocytes were induced with IL-1β, and RAW 264.7 cells were treated with LPS and co-incubated with either individual extracts of PV and GL or different ratios of PV and GL mixture. For the OA animal model, the medial meniscus (DMM) was destabilized in 9-week-old male C57BL/6 mice. Treatment of individual PV and GL and combination of PV and GL extracts inhibited the mRNA expression level of COX2 in chondrocytes and RAW 264.7 cells. The optimized inhibitory effect was attained with a PV and GL combination at an 8:2 ratio (PG) without cytotoxic effects. PG extracts prevented the expression of catabolic factors (COX2, Mmp3, Mmp9, and Mmp13) and inflammatory mediator levels (PGE2 and collagenase). In addition, PG decreased subchondral sclerosis and increased BMD in the subchondral region of DMM-induced OA mice with protection of articular cartilage destruction by inhibiting inflammatory processes. This study suggests that PG may be an alternative medicinal herb for treatment of OA.
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spelling pubmed-98549302023-01-21 Anti-Osteoarthritic Effects of Prunella Vulgaris and Gentiana Lutea In Vitro and In Vivo Kim, Jeonghyun Lee, Chang-Gun Hwang, Seokjin Yun, Seung-Hee Uprety, Laxmi Prasad Oh, Kang-Il Singh, Shivani Yoo, Jisu Jeong, Hyesoo Yong, Yoonjoong Yeo, Subin Park, Eunkuk Jeong, Seon-Yong Antioxidants (Basel) Article Osteoarthritis (OA) is the progressive destruction of articular cartilage with severe symptoms, including pain and stiffness. We investigated the anti-osteoarthritic effects of Prunella vulgaris (PV) and Gentiana lutea (GL) extract in primary cultured chondrocytes RAW 264.7 cells in vitro and destabilization of the medial meniscus (DMM)-induced OA mice in vivo. Primary chondrocytes were induced with IL-1β, and RAW 264.7 cells were treated with LPS and co-incubated with either individual extracts of PV and GL or different ratios of PV and GL mixture. For the OA animal model, the medial meniscus (DMM) was destabilized in 9-week-old male C57BL/6 mice. Treatment of individual PV and GL and combination of PV and GL extracts inhibited the mRNA expression level of COX2 in chondrocytes and RAW 264.7 cells. The optimized inhibitory effect was attained with a PV and GL combination at an 8:2 ratio (PG) without cytotoxic effects. PG extracts prevented the expression of catabolic factors (COX2, Mmp3, Mmp9, and Mmp13) and inflammatory mediator levels (PGE2 and collagenase). In addition, PG decreased subchondral sclerosis and increased BMD in the subchondral region of DMM-induced OA mice with protection of articular cartilage destruction by inhibiting inflammatory processes. This study suggests that PG may be an alternative medicinal herb for treatment of OA. MDPI 2022-12-26 /pmc/articles/PMC9854930/ /pubmed/36670908 http://dx.doi.org/10.3390/antiox12010047 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kim, Jeonghyun
Lee, Chang-Gun
Hwang, Seokjin
Yun, Seung-Hee
Uprety, Laxmi Prasad
Oh, Kang-Il
Singh, Shivani
Yoo, Jisu
Jeong, Hyesoo
Yong, Yoonjoong
Yeo, Subin
Park, Eunkuk
Jeong, Seon-Yong
Anti-Osteoarthritic Effects of Prunella Vulgaris and Gentiana Lutea In Vitro and In Vivo
title Anti-Osteoarthritic Effects of Prunella Vulgaris and Gentiana Lutea In Vitro and In Vivo
title_full Anti-Osteoarthritic Effects of Prunella Vulgaris and Gentiana Lutea In Vitro and In Vivo
title_fullStr Anti-Osteoarthritic Effects of Prunella Vulgaris and Gentiana Lutea In Vitro and In Vivo
title_full_unstemmed Anti-Osteoarthritic Effects of Prunella Vulgaris and Gentiana Lutea In Vitro and In Vivo
title_short Anti-Osteoarthritic Effects of Prunella Vulgaris and Gentiana Lutea In Vitro and In Vivo
title_sort anti-osteoarthritic effects of prunella vulgaris and gentiana lutea in vitro and in vivo
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9854930/
https://www.ncbi.nlm.nih.gov/pubmed/36670908
http://dx.doi.org/10.3390/antiox12010047
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