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Microbial Community Structure among Honey Samples of Different Pollen Origin
Honey’s antibacterial activity has been recently linked to the inhibitory effects of honey microbiota against a range of foodborne and human pathogens. In the current study, the microbial community structure of honey samples exerting pronounced antimicrobial activity was examined. The honey samples...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9855004/ https://www.ncbi.nlm.nih.gov/pubmed/36671302 http://dx.doi.org/10.3390/antibiotics12010101 |
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author | Stavropoulou, Elisavet Remmas, Nikolaos Voidarou, Chrysoula (Chrysa) Vrioni, Georgia Konstantinidis, Theodoros Ntougias, Spyridon Tsakris, Athanasios |
author_facet | Stavropoulou, Elisavet Remmas, Nikolaos Voidarou, Chrysoula (Chrysa) Vrioni, Georgia Konstantinidis, Theodoros Ntougias, Spyridon Tsakris, Athanasios |
author_sort | Stavropoulou, Elisavet |
collection | PubMed |
description | Honey’s antibacterial activity has been recently linked to the inhibitory effects of honey microbiota against a range of foodborne and human pathogens. In the current study, the microbial community structure of honey samples exerting pronounced antimicrobial activity was examined. The honey samples were obtained from different geographical locations in Greece and had diverse pollen origin (fir, cotton, fir–oak, and Arbutus unedo honeys). Identification of honey microbiota was performed by high-throughput amplicon sequencing analysis, detecting 335 distinct taxa in the analyzed samples. Regarding ecological indices, the fir and cotton honeys possessed greater diversity than the fir–oak and Arbutus unedo ones. Lactobacillus kunkeei (basionym of Apilactobacillus kun-keei) was the predominant taxon in the fir honey examined. Lactobacillus spp. appeared to be favored in honey from fir-originated pollen and nectar since lactobacilli were more pronounced in fir compared to fir–oak honey. Pseudomonas, Streptococcus, Lysobacter and Meiothermus were the predominant taxa in cotton honey, whereas Lonsdalea, the causing agent of acute oak decline, and Zymobacter, an osmotolerant facultative anaerobic fermenter, were the dominant taxa in fir–oak honey. Moreover, methylotrophic bacteria represented 1.3–3% of the total relative abundance, independently of the geographical and pollen origin, indicating that methylotrophy plays an important role in honeybee ecology and functionality. A total of 14 taxa were identified in all examined honey samples, including bacilli/anoxybacilli, paracocci, lysobacters, pseudomonads, and sphingomonads. It is concluded that microbial constituents of the honey samples examined were native gut microbiota of melliferous bees and microbiota of their flowering plants, including both beneficial bacteria, such as potential probiotic strains, and animal and plant pathogens, e.g., Staphylococcus spp. and Lonsdalea spp. Further experimentation will elucidate aspects of potential application of microbial bioindicators in identifying the authenticity of honey and honeybee-derived products. |
format | Online Article Text |
id | pubmed-9855004 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-98550042023-01-21 Microbial Community Structure among Honey Samples of Different Pollen Origin Stavropoulou, Elisavet Remmas, Nikolaos Voidarou, Chrysoula (Chrysa) Vrioni, Georgia Konstantinidis, Theodoros Ntougias, Spyridon Tsakris, Athanasios Antibiotics (Basel) Article Honey’s antibacterial activity has been recently linked to the inhibitory effects of honey microbiota against a range of foodborne and human pathogens. In the current study, the microbial community structure of honey samples exerting pronounced antimicrobial activity was examined. The honey samples were obtained from different geographical locations in Greece and had diverse pollen origin (fir, cotton, fir–oak, and Arbutus unedo honeys). Identification of honey microbiota was performed by high-throughput amplicon sequencing analysis, detecting 335 distinct taxa in the analyzed samples. Regarding ecological indices, the fir and cotton honeys possessed greater diversity than the fir–oak and Arbutus unedo ones. Lactobacillus kunkeei (basionym of Apilactobacillus kun-keei) was the predominant taxon in the fir honey examined. Lactobacillus spp. appeared to be favored in honey from fir-originated pollen and nectar since lactobacilli were more pronounced in fir compared to fir–oak honey. Pseudomonas, Streptococcus, Lysobacter and Meiothermus were the predominant taxa in cotton honey, whereas Lonsdalea, the causing agent of acute oak decline, and Zymobacter, an osmotolerant facultative anaerobic fermenter, were the dominant taxa in fir–oak honey. Moreover, methylotrophic bacteria represented 1.3–3% of the total relative abundance, independently of the geographical and pollen origin, indicating that methylotrophy plays an important role in honeybee ecology and functionality. A total of 14 taxa were identified in all examined honey samples, including bacilli/anoxybacilli, paracocci, lysobacters, pseudomonads, and sphingomonads. It is concluded that microbial constituents of the honey samples examined were native gut microbiota of melliferous bees and microbiota of their flowering plants, including both beneficial bacteria, such as potential probiotic strains, and animal and plant pathogens, e.g., Staphylococcus spp. and Lonsdalea spp. Further experimentation will elucidate aspects of potential application of microbial bioindicators in identifying the authenticity of honey and honeybee-derived products. MDPI 2023-01-06 /pmc/articles/PMC9855004/ /pubmed/36671302 http://dx.doi.org/10.3390/antibiotics12010101 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Stavropoulou, Elisavet Remmas, Nikolaos Voidarou, Chrysoula (Chrysa) Vrioni, Georgia Konstantinidis, Theodoros Ntougias, Spyridon Tsakris, Athanasios Microbial Community Structure among Honey Samples of Different Pollen Origin |
title | Microbial Community Structure among Honey Samples of Different Pollen Origin |
title_full | Microbial Community Structure among Honey Samples of Different Pollen Origin |
title_fullStr | Microbial Community Structure among Honey Samples of Different Pollen Origin |
title_full_unstemmed | Microbial Community Structure among Honey Samples of Different Pollen Origin |
title_short | Microbial Community Structure among Honey Samples of Different Pollen Origin |
title_sort | microbial community structure among honey samples of different pollen origin |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9855004/ https://www.ncbi.nlm.nih.gov/pubmed/36671302 http://dx.doi.org/10.3390/antibiotics12010101 |
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