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Application of Tissue Engineering in Manufacturing Absorbable Membranes to Improve the Osteopromoting Potential of Collagen
The membranes are an important biomaterial that contribute to osteopromotion. This study aimed to evaluate the osteopromotive potential of collagen membranes associated with Hydroxyapatite (HA) in critical size calvaria rat’s defects. Ninety-six Albinus Wistar rats were divided into four groups: (CG...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9855111/ https://www.ncbi.nlm.nih.gov/pubmed/36671587 http://dx.doi.org/10.3390/bioengineering10010015 |
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author | de Oliveira, Júlio César Silva Baggio, Ana Maira Pereira Benetti, Luan Pier Delamura, Izabela Fornazari Ramos, Edith Umasi Bizelli, Vinícius Ferreira Bassi, Ana Paula Farnezi |
author_facet | de Oliveira, Júlio César Silva Baggio, Ana Maira Pereira Benetti, Luan Pier Delamura, Izabela Fornazari Ramos, Edith Umasi Bizelli, Vinícius Ferreira Bassi, Ana Paula Farnezi |
author_sort | de Oliveira, Júlio César Silva |
collection | PubMed |
description | The membranes are an important biomaterial that contribute to osteopromotion. This study aimed to evaluate the osteopromotive potential of collagen membranes associated with Hydroxyapatite (HA) in critical size calvaria rat’s defects. Ninety-six Albinus Wistar rats were divided into four groups: (CG) negative control: clot only (CG); positive control: porcine collagen membrane (BG); fish collagen membrane associated with HA (CP); bovine collagen membrane associated with HA (CB), analyzed at 7, 15, 30, and 60 postoperative days. At 30 days, membrane integrity was observed in the CB and fragments in the CP and BG groups were dispersed in the center of the defect. At 60 days, BG demonstrated better results with no statistical difference for the CP group (p = 0.199) and a statistically significant difference for the CB group (p = 0.013). The inflammatory profiles of the BG and CP groups were similar. Immunohistochemistry demonstrated at 60 days moderate osteopontin staining for the BG and CP groups, light staining for the CB, and intense osteocalcin staining for the BG, while the CB and CP groups demonstrated moderate staining. Microtomography revealed the highest mean bone volume (14.247 mm(3)) in the BG, followed by the CB (11.850 mm(3)), and CP (9.560 mm(3)) group. The collagen membranes associated with HA demonstrated an osteopromotive potential. |
format | Online Article Text |
id | pubmed-9855111 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-98551112023-01-21 Application of Tissue Engineering in Manufacturing Absorbable Membranes to Improve the Osteopromoting Potential of Collagen de Oliveira, Júlio César Silva Baggio, Ana Maira Pereira Benetti, Luan Pier Delamura, Izabela Fornazari Ramos, Edith Umasi Bizelli, Vinícius Ferreira Bassi, Ana Paula Farnezi Bioengineering (Basel) Article The membranes are an important biomaterial that contribute to osteopromotion. This study aimed to evaluate the osteopromotive potential of collagen membranes associated with Hydroxyapatite (HA) in critical size calvaria rat’s defects. Ninety-six Albinus Wistar rats were divided into four groups: (CG) negative control: clot only (CG); positive control: porcine collagen membrane (BG); fish collagen membrane associated with HA (CP); bovine collagen membrane associated with HA (CB), analyzed at 7, 15, 30, and 60 postoperative days. At 30 days, membrane integrity was observed in the CB and fragments in the CP and BG groups were dispersed in the center of the defect. At 60 days, BG demonstrated better results with no statistical difference for the CP group (p = 0.199) and a statistically significant difference for the CB group (p = 0.013). The inflammatory profiles of the BG and CP groups were similar. Immunohistochemistry demonstrated at 60 days moderate osteopontin staining for the BG and CP groups, light staining for the CB, and intense osteocalcin staining for the BG, while the CB and CP groups demonstrated moderate staining. Microtomography revealed the highest mean bone volume (14.247 mm(3)) in the BG, followed by the CB (11.850 mm(3)), and CP (9.560 mm(3)) group. The collagen membranes associated with HA demonstrated an osteopromotive potential. MDPI 2022-12-21 /pmc/articles/PMC9855111/ /pubmed/36671587 http://dx.doi.org/10.3390/bioengineering10010015 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article de Oliveira, Júlio César Silva Baggio, Ana Maira Pereira Benetti, Luan Pier Delamura, Izabela Fornazari Ramos, Edith Umasi Bizelli, Vinícius Ferreira Bassi, Ana Paula Farnezi Application of Tissue Engineering in Manufacturing Absorbable Membranes to Improve the Osteopromoting Potential of Collagen |
title | Application of Tissue Engineering in Manufacturing Absorbable Membranes to Improve the Osteopromoting Potential of Collagen |
title_full | Application of Tissue Engineering in Manufacturing Absorbable Membranes to Improve the Osteopromoting Potential of Collagen |
title_fullStr | Application of Tissue Engineering in Manufacturing Absorbable Membranes to Improve the Osteopromoting Potential of Collagen |
title_full_unstemmed | Application of Tissue Engineering in Manufacturing Absorbable Membranes to Improve the Osteopromoting Potential of Collagen |
title_short | Application of Tissue Engineering in Manufacturing Absorbable Membranes to Improve the Osteopromoting Potential of Collagen |
title_sort | application of tissue engineering in manufacturing absorbable membranes to improve the osteopromoting potential of collagen |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9855111/ https://www.ncbi.nlm.nih.gov/pubmed/36671587 http://dx.doi.org/10.3390/bioengineering10010015 |
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