Cargando…

Characterisation of Methicillin-Resistant Staphylococcus aureus from Alexandria, Egypt

The present study aims to characterise clinical MRSA isolates from a tertiary care centre in Egypt’s second-largest city, Alexandria. Thirty isolates collected in 2020 were genotypically characterised by microarray to detect their resistance and virulence genes and assign them to clonal complexes (C...

Descripción completa

Detalles Bibliográficos
Autores principales: Monecke, Stefan, Bedewy, Amira K., Müller, Elke, Braun, Sascha D., Diezel, Celia, Elsheredy, Amel, Kader, Ola, Reinicke, Martin, Ghazal, Abeer, Rezk, Shahinda, Ehricht, Ralf
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9855118/
https://www.ncbi.nlm.nih.gov/pubmed/36671279
http://dx.doi.org/10.3390/antibiotics12010078
_version_ 1784873300639350784
author Monecke, Stefan
Bedewy, Amira K.
Müller, Elke
Braun, Sascha D.
Diezel, Celia
Elsheredy, Amel
Kader, Ola
Reinicke, Martin
Ghazal, Abeer
Rezk, Shahinda
Ehricht, Ralf
author_facet Monecke, Stefan
Bedewy, Amira K.
Müller, Elke
Braun, Sascha D.
Diezel, Celia
Elsheredy, Amel
Kader, Ola
Reinicke, Martin
Ghazal, Abeer
Rezk, Shahinda
Ehricht, Ralf
author_sort Monecke, Stefan
collection PubMed
description The present study aims to characterise clinical MRSA isolates from a tertiary care centre in Egypt’s second-largest city, Alexandria. Thirty isolates collected in 2020 were genotypically characterised by microarray to detect their resistance and virulence genes and assign them to clonal complexes (CC) and strains. Isolates belonged to 11 different CCs and 14 different strains. CC15-MRSA-[V+fus] (n = 6), CC1-MRSA-[V+fus+tir+ccrA/B-1] (PVL+) (n = 5) as well as CC1-MRSA-[V+fus+tir+ccrA/B-1] and CC1153-MRSA-[V+fus] (PVL+) (both with n = 3) were the most common strains. Most isolates (83%) harboured variant or composite SCCmec V or VI elements that included the fusidic acid resistance gene fusC. The SCCmec [V+fus+tir+ccrA/B-1] element of one of the CC1 isolates was sequenced, revealing a presence not only of fusC but also of blaZ, aacA-aphD and other resistance genes. PVL genes were also common (40%). The hospital-acquired MRSA CC239-III strain was only found twice. A comparison to data from a study on strains collected in 2015 (Montelongo et al., 2022) showed an increase in fusC and PVL carriage and a decreasing prevalence of the CC239 strain. These observations indicate a diffusion of community-acquired strains into hospital settings. The beta-lactam use in hospitals and the widespread fusidic acid consumption in the community might pose a selective pressure that favours MRSA strains with composite SCCmec elements comprising mecA and fusC. This is an unsettling trend, but more MRSA typing data from Egypt are required.
format Online
Article
Text
id pubmed-9855118
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-98551182023-01-21 Characterisation of Methicillin-Resistant Staphylococcus aureus from Alexandria, Egypt Monecke, Stefan Bedewy, Amira K. Müller, Elke Braun, Sascha D. Diezel, Celia Elsheredy, Amel Kader, Ola Reinicke, Martin Ghazal, Abeer Rezk, Shahinda Ehricht, Ralf Antibiotics (Basel) Article The present study aims to characterise clinical MRSA isolates from a tertiary care centre in Egypt’s second-largest city, Alexandria. Thirty isolates collected in 2020 were genotypically characterised by microarray to detect their resistance and virulence genes and assign them to clonal complexes (CC) and strains. Isolates belonged to 11 different CCs and 14 different strains. CC15-MRSA-[V+fus] (n = 6), CC1-MRSA-[V+fus+tir+ccrA/B-1] (PVL+) (n = 5) as well as CC1-MRSA-[V+fus+tir+ccrA/B-1] and CC1153-MRSA-[V+fus] (PVL+) (both with n = 3) were the most common strains. Most isolates (83%) harboured variant or composite SCCmec V or VI elements that included the fusidic acid resistance gene fusC. The SCCmec [V+fus+tir+ccrA/B-1] element of one of the CC1 isolates was sequenced, revealing a presence not only of fusC but also of blaZ, aacA-aphD and other resistance genes. PVL genes were also common (40%). The hospital-acquired MRSA CC239-III strain was only found twice. A comparison to data from a study on strains collected in 2015 (Montelongo et al., 2022) showed an increase in fusC and PVL carriage and a decreasing prevalence of the CC239 strain. These observations indicate a diffusion of community-acquired strains into hospital settings. The beta-lactam use in hospitals and the widespread fusidic acid consumption in the community might pose a selective pressure that favours MRSA strains with composite SCCmec elements comprising mecA and fusC. This is an unsettling trend, but more MRSA typing data from Egypt are required. MDPI 2023-01-01 /pmc/articles/PMC9855118/ /pubmed/36671279 http://dx.doi.org/10.3390/antibiotics12010078 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Monecke, Stefan
Bedewy, Amira K.
Müller, Elke
Braun, Sascha D.
Diezel, Celia
Elsheredy, Amel
Kader, Ola
Reinicke, Martin
Ghazal, Abeer
Rezk, Shahinda
Ehricht, Ralf
Characterisation of Methicillin-Resistant Staphylococcus aureus from Alexandria, Egypt
title Characterisation of Methicillin-Resistant Staphylococcus aureus from Alexandria, Egypt
title_full Characterisation of Methicillin-Resistant Staphylococcus aureus from Alexandria, Egypt
title_fullStr Characterisation of Methicillin-Resistant Staphylococcus aureus from Alexandria, Egypt
title_full_unstemmed Characterisation of Methicillin-Resistant Staphylococcus aureus from Alexandria, Egypt
title_short Characterisation of Methicillin-Resistant Staphylococcus aureus from Alexandria, Egypt
title_sort characterisation of methicillin-resistant staphylococcus aureus from alexandria, egypt
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9855118/
https://www.ncbi.nlm.nih.gov/pubmed/36671279
http://dx.doi.org/10.3390/antibiotics12010078
work_keys_str_mv AT moneckestefan characterisationofmethicillinresistantstaphylococcusaureusfromalexandriaegypt
AT bedewyamirak characterisationofmethicillinresistantstaphylococcusaureusfromalexandriaegypt
AT mullerelke characterisationofmethicillinresistantstaphylococcusaureusfromalexandriaegypt
AT braunsaschad characterisationofmethicillinresistantstaphylococcusaureusfromalexandriaegypt
AT diezelcelia characterisationofmethicillinresistantstaphylococcusaureusfromalexandriaegypt
AT elsheredyamel characterisationofmethicillinresistantstaphylococcusaureusfromalexandriaegypt
AT kaderola characterisationofmethicillinresistantstaphylococcusaureusfromalexandriaegypt
AT reinickemartin characterisationofmethicillinresistantstaphylococcusaureusfromalexandriaegypt
AT ghazalabeer characterisationofmethicillinresistantstaphylococcusaureusfromalexandriaegypt
AT rezkshahinda characterisationofmethicillinresistantstaphylococcusaureusfromalexandriaegypt
AT ehrichtralf characterisationofmethicillinresistantstaphylococcusaureusfromalexandriaegypt