Cargando…

Optimizing the Seeding Density of Human Mononuclear Cells to Improve the Purity of Highly Proliferative Mesenchymal Stem Cells

Mesenchymal stem cells (MSCs) hold considerable promise for regenerative medicine. Optimization of the seeding density of mononuclear cells (MNCs) improves the proliferative and differentiation potential of isolated MSCs. However, the underlying mechanism is unclear. We cultured human bone marrow MN...

Descripción completa

Detalles Bibliográficos
Autores principales:	Nagai, Hiroyuki, Miwa, Akihiro, Yoneda, Kenji, Fujisawa, Koichi, Takami, Taro
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2023
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9855129/ https://www.ncbi.nlm.nih.gov/pubmed/36671674 http://dx.doi.org/10.3390/bioengineering10010102

Ejemplares similares

Evaluation of the effects of ascorbic acid on metabolism of human mesenchymal stem cells
por: Fujisawa, Koichi, et al.
Publicado: (2018)

Trans-portal hepatic infusion of cultured bone marrow-derived mesenchymal stem cells in a steatohepatitis murine model
por: Sasaki, Ryo, et al.
Publicado: (2020)

The proliferative effects of asbestos-exposed peripheral blood mononuclear cells on mesothelial cells
por: MAKI, YUHO, et al.
Publicado: (2016)

NUPR1 acts as a pro-survival factor in human bone marrow-derived mesenchymal stem cells and is induced by the hypoxia mimetic reagent deferoxamine
por: Matsunaga, Kazuhito, et al.
Publicado: (2019)

Metabolic Analysis of DFO-Resistant Huh7 Cells and Identification of Targets for Combination Therapy
por: Fujisawa, Koichi, et al.
Publicado: (2023)

Metabolic Alterations in Spheroid-Cultured Hepatic Stellate Cells
por: Fujisawa, Koichi, et al.
Publicado: (2020)

Effects of cell seeding density on real-time monitoring of anti-proliferative effects of transient gene silencing
por: Selli, Cigdem, et al.
Publicado: (2016)

Purity Determines the Effect of Extracellular Vesicles Derived from Mesenchymal Stromal Cells
por: Forteza-Genestra, Maria Antònia, et al.
Publicado: (2020)

An iron chelation-based combinatorial anticancer therapy comprising deferoxamine and a lactate excretion inhibitor inhibits the proliferation of cancer cells
por: Fujisawa, Koichi, et al.
Publicado: (2022)

Effects of anticancer agents on cell viability, proliferative activity and cytokine production of peripheral blood mononuclear cells
por: Sakai, Hiromi, et al.
Publicado: (2013)

Adenylate Kinase Isozyme 3 Regulates Mitochondrial Energy Metabolism and Knockout Alters HeLa Cell Metabolism
por: Fujisawa, Koichi, et al.
Publicado: (2022)

Bone marrow-derived cells for cardiovascular cell therapy: an optimized GMP method based on low-density gradient improves cell purity and function
por: Radrizzani, Marina, et al.
Publicado: (2014)

Iodine Absorption Cells Purity Testing
por: Hrabina, Jan, et al.
Publicado: (2017)

Novel low shear 3D bioreactor for high purity mesenchymal stem cell production
por: Burns, Andrew B., et al.
Publicado: (2021)

Extended bioreactor conditioning of mononuclear cell–seeded heart valve scaffolds
por: VeDepo, Mitchell, et al.
Publicado: (2018)

Cytokine Secretion of Peripheral Blood Mononuclear Cells by Hydnocarpus anthelminthicus Seeds
por: Leelawat, Surang, et al.
Publicado: (2018)

Profiling of the circadian metabolome in thioacetamide‐induced liver cirrhosis in mice
por: Fujisawa, Koichi, et al.
Publicado: (2017)

Initial cell density encodes proliferative potential in cancer cell populations
por: Enrico Bena, Chiara, et al.
Publicado: (2021)

A TNFR2-Agonist Facilitates High Purity Expansion of Human Low Purity Treg Cells
por: He, Xuehui, et al.
Publicado: (2016)

Liver regeneration therapy through the hepatic artery-infusion of cultured bone marrow cells in a canine liver fibrosis model
por: Nishimura, Tatsuro, et al.
Publicado: (2019)

Response of Human Mesenchymal Stromal Cells from Periodontal Tissue to LPS Depends on the Purity but Not on the LPS Source
por: Behm, Christian, et al.
Publicado: (2020)

Isolation of High Purity Mouse Mesenchymal Stem Cells through Depleting Macrophages Using Liposomal Clodronate
por: Song, Ju Han, et al.
Publicado: (2022)

The Purity of Milk
Publicado: (1898)

Evaluation of the Effects of Microgravity on Activated Primary Human Hepatic Stellate Cells
por: Fujisawa, Koichi, et al.
Publicado: (2022)

Detection of seed purity of hybrid wheat using reflectance and transmittance hyperspectral imaging technology
por: Zhang, Han, et al.
Publicado: (2022)

Effects of cell seeding technique and cell density on BMP‐2 production in transduced human mesenchymal stem cells
por: Collon, Kevin, et al.
Publicado: (2022)

Density Gradient Centrifugation Compromises Bone Marrow Mononuclear Cell Yield
por: Pösel, Claudia, et al.
Publicado: (2012)

Effectiveness of tolvaptan monotherapy and low-dose furosemide/tolvaptan combination therapy for hepatoprotection and diuresis in a rat cirrhotic model
por: Tanabe, Norikazu, et al.
Publicado: (2017)

Proliferative Cell Index in Endometrial Adenocarcinoma of Different Nuclear Grades
por: Mushika, Masafumi, et al.
Publicado: (1989)

Increased telomere length and proliferative potential in peripheral blood mononuclear cells of adults of different ages stimulated with concanavalin A
por: Murillo-Ortiz, Blanca, et al.
Publicado: (2013)

Efficacy of optimized in vitro predegeneration period on the cell count and purity of canine Schwann cell cultures
por: Niapour, Nazila, et al.
Publicado: (2015)

Supernatant of Bone Marrow Mesenchymal Stromal Cells Induces Peripheral Blood Mononuclear Cells Possessing Mesenchymal Features
por: Hu, Gang, et al.
Publicado: (2011)

Improvement in Purity of Healthy Tomato Seeds Using an Image-Based One-Class Classification Method
por: Yasmin, Jannat, et al.
Publicado: (2020)

Rice Seed Purity Identification Technology Using Hyperspectral Image with LASSO Logistic Regression Model
por: Liu, Weihua, et al.
Publicado: (2021)

Cell culture and purification process — purity and safety testing
por: Koplove, H. M.
Publicado: (1994)

ARTEMIS stabilizes the genome and modulates proliferative responses in multipotent mesenchymal cells
por: Maas, Sarah A, et al.
Publicado: (2010)

Gelatin Nanoparticle-Coated Silicon Beads for Density-Selective Capture and Release of Heterogeneous Circulating Tumor Cells with High Purity
por: Huang, Qinqin, et al.
Publicado: (2018)

Bone marrow non-mesenchymal mononuclear cells induce functional differentiation of neuroblastoma cells
por: Phruksaniyom, Chareerut, et al.
Publicado: (2013)

Comparative analysis of canine mesenchymal stem cells and bone marrow-derived mononuclear cells
por: Maeta, Noritaka, et al.
Publicado: (2021)

Purity, spectra and localisation
por: Prest, Mike
Publicado: (2009)

Cannot write session to /tmp/vufind_sessions/sess_g210k8br17s1gmqdmfr64s5ecc