Effect of Additives Inclusion in Gilthead Seabream (Sparus aurata L.) Diets on Growth, Enzyme Activity, Digestibility and Gut Histology Fed with Vegetable Meals

SIMPLE SUMMARY: Aquaculture is currently directing its efforts towards the principles of sustainability, especially in the reduction in the use of fishmeal, which is why it faces a constant search for alternative sources. In the current work, it has been found that the use of two different feed additives, hydrolyzed porcine mucosa and nucleotide concentrate, in different percentages in a plant-based diet for gilthead seabream, improves growth and feed efficiency. ABSTRACT: The fishmeal replacement by vegetable meals or other alternative sources, without affecting fish performance and productivity, is one of the principal challenges in aquaculture. The use of hydrolyzed porcine mucosa (HPM) and nucleotide (NT) concentrates, as feed additives in gilthead seabream (Sparus aurata L.) non-fishmeal diets was assessed in order to determine the possible effects on growth, feed efficiency, protein digestion, and gut histology when these were included in a plant-based diet (HPM 1% and 2%, P1 and P2; NT 250 and 500 ppm, N250 and N500), in comparison with two control diets, AA0 (100% plant-protein-based diet) and FM100 (100% fishmeal-protein-based diet). Diets were assayed in triplicate and the growth assay lasted 134 days. Results showed a significant improvement in all groups in terms of final weight and specific growth rate in comparison with the AA0 group. An improvement in the feed conversion ratio and the protein efficiency ratio was also observed when the additives were included in lower percentages (P1 and N250) compared to the FM100 group. Significant differences were found in hepatosomatic index, villi thickness, and goblet cells. Thus, the inclusion of NT and HPM was tested as beneficial for the improvement of efficiency of plant feed in seabream.