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Association of SRXN1 Receptor Gene Polymorphism with Susceptibility to Periodontitis

BACKGROUND: Emerging evidence suggests that oxidative stress forms a key component in the etiopathogenesis of periodontitis. Literature evidence have shown potential antioxidants responsible for combating the pro-oxidants which stress the periodontium, but the peroxiredoxin-sulfiredoxin system is ex...

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Autores principales: Murthykumar, Karthikeyan, Varghese, Sheeja, Jayaseelan, Vijayashree Priyadharsini
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wolters Kluwer - Medknow 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9855261/
https://www.ncbi.nlm.nih.gov/pubmed/36686993
http://dx.doi.org/10.4103/ccd.ccd_309_21
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author Murthykumar, Karthikeyan
Varghese, Sheeja
Jayaseelan, Vijayashree Priyadharsini
author_facet Murthykumar, Karthikeyan
Varghese, Sheeja
Jayaseelan, Vijayashree Priyadharsini
author_sort Murthykumar, Karthikeyan
collection PubMed
description BACKGROUND: Emerging evidence suggests that oxidative stress forms a key component in the etiopathogenesis of periodontitis. Literature evidence have shown potential antioxidants responsible for combating the pro-oxidants which stress the periodontium, but the peroxiredoxin-sulfiredoxin system is explored very minimally in periodontal disease. Thus, the present study was aimed to evaluate the genetic association of SRXN1 receptor gene polymorphism (rs6053666). MATERIALS AND METHODS: A total of 100 subjects were recruited for this study, which included 50 Periodontitis patients (Stage II and above based on the criteria of American Association of Periodontology-2018) and 50 periodontally healthy or mild gingivitis. Genomic DNA was extracted from the whole blood collected from the subjects. DNA was amplified using specific primers flanking the BtgI region of the SRXN1 receptor gene. The amplicon was further subjected to genotyping using restriction fragment length using BtgI enzyme. The genotype obtained based on the restriction fragment length polymorphism pattern was recorded and used for statistical analysis. The distribution of genotypes and allele frequencies in the periodontitis and control groups were compared using the Chi-square test. The risk associated with individual alleles or genotypes was calculated as the odds ratio with 95% confidence intervals. Statistical significance in all tests was determined at P < 0.05. RESULTS: The genotype frequency and distributions of SRXN1 receptor BtgI polymorphism did not differ significantly at ꭕ2df (P = 0.557). Our study results showed that homozygous and heterozygous mutant genotypes had no significant difference (CC vs. CT + TT) between the periodontitis patients and control group with a P = 0.4266. The detected frequency of CT (38% vs. 34%) and TT (42% vs. 52%) genotype showed no significant difference between control and test group. There was no significant difference in C allele (39% vs. 31%) and T allele (61% vs. 69%) between the test and control group. CONCLUSION: The present study denotes that SRXN1 receptor gene polymorphism is not associated with periodontitis in the study group analyzed.
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spelling pubmed-98552612023-01-21 Association of SRXN1 Receptor Gene Polymorphism with Susceptibility to Periodontitis Murthykumar, Karthikeyan Varghese, Sheeja Jayaseelan, Vijayashree Priyadharsini Contemp Clin Dent Original Article BACKGROUND: Emerging evidence suggests that oxidative stress forms a key component in the etiopathogenesis of periodontitis. Literature evidence have shown potential antioxidants responsible for combating the pro-oxidants which stress the periodontium, but the peroxiredoxin-sulfiredoxin system is explored very minimally in periodontal disease. Thus, the present study was aimed to evaluate the genetic association of SRXN1 receptor gene polymorphism (rs6053666). MATERIALS AND METHODS: A total of 100 subjects were recruited for this study, which included 50 Periodontitis patients (Stage II and above based on the criteria of American Association of Periodontology-2018) and 50 periodontally healthy or mild gingivitis. Genomic DNA was extracted from the whole blood collected from the subjects. DNA was amplified using specific primers flanking the BtgI region of the SRXN1 receptor gene. The amplicon was further subjected to genotyping using restriction fragment length using BtgI enzyme. The genotype obtained based on the restriction fragment length polymorphism pattern was recorded and used for statistical analysis. The distribution of genotypes and allele frequencies in the periodontitis and control groups were compared using the Chi-square test. The risk associated with individual alleles or genotypes was calculated as the odds ratio with 95% confidence intervals. Statistical significance in all tests was determined at P < 0.05. RESULTS: The genotype frequency and distributions of SRXN1 receptor BtgI polymorphism did not differ significantly at ꭕ2df (P = 0.557). Our study results showed that homozygous and heterozygous mutant genotypes had no significant difference (CC vs. CT + TT) between the periodontitis patients and control group with a P = 0.4266. The detected frequency of CT (38% vs. 34%) and TT (42% vs. 52%) genotype showed no significant difference between control and test group. There was no significant difference in C allele (39% vs. 31%) and T allele (61% vs. 69%) between the test and control group. CONCLUSION: The present study denotes that SRXN1 receptor gene polymorphism is not associated with periodontitis in the study group analyzed. Wolters Kluwer - Medknow 2022 2022-11-03 /pmc/articles/PMC9855261/ /pubmed/36686993 http://dx.doi.org/10.4103/ccd.ccd_309_21 Text en Copyright: © 2022 Contemporary Clinical Dentistry https://creativecommons.org/licenses/by-nc-sa/4.0/This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.
spellingShingle Original Article
Murthykumar, Karthikeyan
Varghese, Sheeja
Jayaseelan, Vijayashree Priyadharsini
Association of SRXN1 Receptor Gene Polymorphism with Susceptibility to Periodontitis
title Association of SRXN1 Receptor Gene Polymorphism with Susceptibility to Periodontitis
title_full Association of SRXN1 Receptor Gene Polymorphism with Susceptibility to Periodontitis
title_fullStr Association of SRXN1 Receptor Gene Polymorphism with Susceptibility to Periodontitis
title_full_unstemmed Association of SRXN1 Receptor Gene Polymorphism with Susceptibility to Periodontitis
title_short Association of SRXN1 Receptor Gene Polymorphism with Susceptibility to Periodontitis
title_sort association of srxn1 receptor gene polymorphism with susceptibility to periodontitis
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9855261/
https://www.ncbi.nlm.nih.gov/pubmed/36686993
http://dx.doi.org/10.4103/ccd.ccd_309_21
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