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A Novel Microfluidic-Based OMC-PEDOT-PSS Composite Electrochemical Sensor for Continuous Dopamine Monitoring

Fast and precise analysis techniques using small sample volumes are required for next-generation clinical monitoring at the patient’s bedside, so as to provide the clinician with relevant chemical data in real-time. The integration of an electrochemical sensor into a microfluidic chip allows for the...

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Autores principales: Nuh, Sofwan, Numnuam, Apon, Thavarungkul, Panote, Phairatana, Tonghathai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9855352/
https://www.ncbi.nlm.nih.gov/pubmed/36671903
http://dx.doi.org/10.3390/bios13010068
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author Nuh, Sofwan
Numnuam, Apon
Thavarungkul, Panote
Phairatana, Tonghathai
author_facet Nuh, Sofwan
Numnuam, Apon
Thavarungkul, Panote
Phairatana, Tonghathai
author_sort Nuh, Sofwan
collection PubMed
description Fast and precise analysis techniques using small sample volumes are required for next-generation clinical monitoring at the patient’s bedside, so as to provide the clinician with relevant chemical data in real-time. The integration of an electrochemical sensor into a microfluidic chip allows for the achievement of real-time chemical monitoring due to the low consumption of analytes, short analysis time, low cost, and compact size. In this work, dopamine, used as a model, is an important neurotransmitter responsible for controlling various vital life functions. The aim is to develop a novel serpentine microfluidic-based electrochemical sensor, using a screen-printed electrode for continuous dopamine detection. The developed sensor employed the composite of ordered mesoporous carbon (OMC) and poly (3,4 ethylenedioxythiophene)-poly (styrene sulfonate) (PEDOT-PSS). The performance of a microfluidic, integrated with the sensor, was amperometrically evaluated using a computer-controlled microfluidic platform. The microfluidic-based dopamine sensor exhibited a sensitivity of 20.2 ± 0.6 μA μmol L(−1), and a detection limit (LOD) of 21.6 ± 0.002 nmol L(−1), with high selectivity. This microfluidic-based electrochemical sensor was successfully employed to determine dopamine continuously, which could overcome the problem of sensor fouling with more than 90% stability for over 24 h. This novel microfluidic sensor platform provides a powerful tool for the development of a continuous dopamine detection system for human clinical application.
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spelling pubmed-98553522023-01-21 A Novel Microfluidic-Based OMC-PEDOT-PSS Composite Electrochemical Sensor for Continuous Dopamine Monitoring Nuh, Sofwan Numnuam, Apon Thavarungkul, Panote Phairatana, Tonghathai Biosensors (Basel) Article Fast and precise analysis techniques using small sample volumes are required for next-generation clinical monitoring at the patient’s bedside, so as to provide the clinician with relevant chemical data in real-time. The integration of an electrochemical sensor into a microfluidic chip allows for the achievement of real-time chemical monitoring due to the low consumption of analytes, short analysis time, low cost, and compact size. In this work, dopamine, used as a model, is an important neurotransmitter responsible for controlling various vital life functions. The aim is to develop a novel serpentine microfluidic-based electrochemical sensor, using a screen-printed electrode for continuous dopamine detection. The developed sensor employed the composite of ordered mesoporous carbon (OMC) and poly (3,4 ethylenedioxythiophene)-poly (styrene sulfonate) (PEDOT-PSS). The performance of a microfluidic, integrated with the sensor, was amperometrically evaluated using a computer-controlled microfluidic platform. The microfluidic-based dopamine sensor exhibited a sensitivity of 20.2 ± 0.6 μA μmol L(−1), and a detection limit (LOD) of 21.6 ± 0.002 nmol L(−1), with high selectivity. This microfluidic-based electrochemical sensor was successfully employed to determine dopamine continuously, which could overcome the problem of sensor fouling with more than 90% stability for over 24 h. This novel microfluidic sensor platform provides a powerful tool for the development of a continuous dopamine detection system for human clinical application. MDPI 2022-12-31 /pmc/articles/PMC9855352/ /pubmed/36671903 http://dx.doi.org/10.3390/bios13010068 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Nuh, Sofwan
Numnuam, Apon
Thavarungkul, Panote
Phairatana, Tonghathai
A Novel Microfluidic-Based OMC-PEDOT-PSS Composite Electrochemical Sensor for Continuous Dopamine Monitoring
title A Novel Microfluidic-Based OMC-PEDOT-PSS Composite Electrochemical Sensor for Continuous Dopamine Monitoring
title_full A Novel Microfluidic-Based OMC-PEDOT-PSS Composite Electrochemical Sensor for Continuous Dopamine Monitoring
title_fullStr A Novel Microfluidic-Based OMC-PEDOT-PSS Composite Electrochemical Sensor for Continuous Dopamine Monitoring
title_full_unstemmed A Novel Microfluidic-Based OMC-PEDOT-PSS Composite Electrochemical Sensor for Continuous Dopamine Monitoring
title_short A Novel Microfluidic-Based OMC-PEDOT-PSS Composite Electrochemical Sensor for Continuous Dopamine Monitoring
title_sort novel microfluidic-based omc-pedot-pss composite electrochemical sensor for continuous dopamine monitoring
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9855352/
https://www.ncbi.nlm.nih.gov/pubmed/36671903
http://dx.doi.org/10.3390/bios13010068
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