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Sesamin Attenuates VEGFA-Induced Angiogenesis via Inhibition of Src and FAK Signaling in Chick Chorioallantoic Membrane Model and Human Endothelial EA.hy926 Cells

Sesamin, a major phytochemical in sesame seeds and oil, has been reported to have effects on physiological and pathological angiogenesis in several studies. Nevertheless, the underlying mechanisms of sesamin’s effect on angiogenesis are not understood well enough. This study aimed to investigate its...

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Autores principales: Keratibumrungpong, Tanyaporn, Srisuthtayanont, Warunee, Wanachewin, Orawan, Klangjorhor, Jeerawan, Phitak, Thanyaluck, Pothacharoen, Peraphan, Shwe, Thuzar Hla, Kongtawelert, Prachya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9855418/
https://www.ncbi.nlm.nih.gov/pubmed/36672695
http://dx.doi.org/10.3390/biomedicines11010188
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author Keratibumrungpong, Tanyaporn
Srisuthtayanont, Warunee
Wanachewin, Orawan
Klangjorhor, Jeerawan
Phitak, Thanyaluck
Pothacharoen, Peraphan
Shwe, Thuzar Hla
Kongtawelert, Prachya
author_facet Keratibumrungpong, Tanyaporn
Srisuthtayanont, Warunee
Wanachewin, Orawan
Klangjorhor, Jeerawan
Phitak, Thanyaluck
Pothacharoen, Peraphan
Shwe, Thuzar Hla
Kongtawelert, Prachya
author_sort Keratibumrungpong, Tanyaporn
collection PubMed
description Sesamin, a major phytochemical in sesame seeds and oil, has been reported to have effects on physiological and pathological angiogenesis in several studies. Nevertheless, the underlying mechanisms of sesamin’s effect on angiogenesis are not understood well enough. This study aimed to investigate its effect on both physiological and pathological angiogenesis using the in vivo chick chorioallantoic membrane (CAM) model and the in vitro human endothelial cell line, EA.hy926, model. Sesamin inhibited the VEGFA-induced pathological angiogenesis significantly, although no effect was seen on angiogenesis without induction. It reduced the formation of vascular branches in the VEGFA-treated CAMs and also the proliferation and migration of EA.hy926 endothelial cells induced by VEGFA. Sesamin impeded the VEGF-mediated activation of Src and FAK signaling proteins, which may be responsible for sesamin-mediated reduction of pathological angiogenesis. Moreover, the effect of sesamin on the expressions of angiogenesis-related genes was then investigated and it was found that both mRNA and protein expressions of Notch1, the key pathway in vascular development, induced by VEGFA, were significantly reduced by sesamin. Our results altogether suggested that sesamin, by inhibiting pathological angiogenesis, has the potential to be employed in the prevention or treatment of diseases with over-angiogenesis, such as cancers.
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spelling pubmed-98554182023-01-21 Sesamin Attenuates VEGFA-Induced Angiogenesis via Inhibition of Src and FAK Signaling in Chick Chorioallantoic Membrane Model and Human Endothelial EA.hy926 Cells Keratibumrungpong, Tanyaporn Srisuthtayanont, Warunee Wanachewin, Orawan Klangjorhor, Jeerawan Phitak, Thanyaluck Pothacharoen, Peraphan Shwe, Thuzar Hla Kongtawelert, Prachya Biomedicines Article Sesamin, a major phytochemical in sesame seeds and oil, has been reported to have effects on physiological and pathological angiogenesis in several studies. Nevertheless, the underlying mechanisms of sesamin’s effect on angiogenesis are not understood well enough. This study aimed to investigate its effect on both physiological and pathological angiogenesis using the in vivo chick chorioallantoic membrane (CAM) model and the in vitro human endothelial cell line, EA.hy926, model. Sesamin inhibited the VEGFA-induced pathological angiogenesis significantly, although no effect was seen on angiogenesis without induction. It reduced the formation of vascular branches in the VEGFA-treated CAMs and also the proliferation and migration of EA.hy926 endothelial cells induced by VEGFA. Sesamin impeded the VEGF-mediated activation of Src and FAK signaling proteins, which may be responsible for sesamin-mediated reduction of pathological angiogenesis. Moreover, the effect of sesamin on the expressions of angiogenesis-related genes was then investigated and it was found that both mRNA and protein expressions of Notch1, the key pathway in vascular development, induced by VEGFA, were significantly reduced by sesamin. Our results altogether suggested that sesamin, by inhibiting pathological angiogenesis, has the potential to be employed in the prevention or treatment of diseases with over-angiogenesis, such as cancers. MDPI 2023-01-11 /pmc/articles/PMC9855418/ /pubmed/36672695 http://dx.doi.org/10.3390/biomedicines11010188 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Keratibumrungpong, Tanyaporn
Srisuthtayanont, Warunee
Wanachewin, Orawan
Klangjorhor, Jeerawan
Phitak, Thanyaluck
Pothacharoen, Peraphan
Shwe, Thuzar Hla
Kongtawelert, Prachya
Sesamin Attenuates VEGFA-Induced Angiogenesis via Inhibition of Src and FAK Signaling in Chick Chorioallantoic Membrane Model and Human Endothelial EA.hy926 Cells
title Sesamin Attenuates VEGFA-Induced Angiogenesis via Inhibition of Src and FAK Signaling in Chick Chorioallantoic Membrane Model and Human Endothelial EA.hy926 Cells
title_full Sesamin Attenuates VEGFA-Induced Angiogenesis via Inhibition of Src and FAK Signaling in Chick Chorioallantoic Membrane Model and Human Endothelial EA.hy926 Cells
title_fullStr Sesamin Attenuates VEGFA-Induced Angiogenesis via Inhibition of Src and FAK Signaling in Chick Chorioallantoic Membrane Model and Human Endothelial EA.hy926 Cells
title_full_unstemmed Sesamin Attenuates VEGFA-Induced Angiogenesis via Inhibition of Src and FAK Signaling in Chick Chorioallantoic Membrane Model and Human Endothelial EA.hy926 Cells
title_short Sesamin Attenuates VEGFA-Induced Angiogenesis via Inhibition of Src and FAK Signaling in Chick Chorioallantoic Membrane Model and Human Endothelial EA.hy926 Cells
title_sort sesamin attenuates vegfa-induced angiogenesis via inhibition of src and fak signaling in chick chorioallantoic membrane model and human endothelial ea.hy926 cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9855418/
https://www.ncbi.nlm.nih.gov/pubmed/36672695
http://dx.doi.org/10.3390/biomedicines11010188
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