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New Data on the Features of Skin Barrier in Hidradenitis Suppurativa

Hidradenitis suppurativa (HS) is a Th1/17-driven inflammatory skin disease of the apocrine gland-rich (AGR) skin regions, where keratinocytes seem to be the crucial drivers of the initial pathogenic steps. However, the possible role of permeability barrier alteration in activating keratinocytes duri...

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Autores principales: Somogyi, Orsolya, Dajnoki, Zsolt, Szabó, Lilla, Gáspár, Krisztián, Hendrik, Zoltán, Zouboulis, Christos C., Dócs, Klaudia, Szücs, Péter, Dull, Katalin, Törőcsik, Dániel, Kapitány, Anikó, Szegedi, Andrea
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9855647/
https://www.ncbi.nlm.nih.gov/pubmed/36672635
http://dx.doi.org/10.3390/biomedicines11010127
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author Somogyi, Orsolya
Dajnoki, Zsolt
Szabó, Lilla
Gáspár, Krisztián
Hendrik, Zoltán
Zouboulis, Christos C.
Dócs, Klaudia
Szücs, Péter
Dull, Katalin
Törőcsik, Dániel
Kapitány, Anikó
Szegedi, Andrea
author_facet Somogyi, Orsolya
Dajnoki, Zsolt
Szabó, Lilla
Gáspár, Krisztián
Hendrik, Zoltán
Zouboulis, Christos C.
Dócs, Klaudia
Szücs, Péter
Dull, Katalin
Törőcsik, Dániel
Kapitány, Anikó
Szegedi, Andrea
author_sort Somogyi, Orsolya
collection PubMed
description Hidradenitis suppurativa (HS) is a Th1/17-driven inflammatory skin disease of the apocrine gland-rich (AGR) skin regions, where keratinocytes seem to be the crucial drivers of the initial pathogenic steps. However, the possible role of permeability barrier alteration in activating keratinocytes during HS development has not been clarified. We compared the major permeability barrier elements of non-lesional HS (HS-NL; n = 10) and lesional HS (HS-L; n = 10) skin with healthy AGR regions (n = 10) via RT-qPCR and immunohistochemistry. Stratum corneum components related to cornified envelope formation, corneocyte desquamation and (corneo)desmosome organization were analyzed along with tight junction molecules and barrier alarmins. The permeability barrier function was also investigated with transepidermal water loss (TEWL) measurements (n = 16). Junction structures were also visualized using confocal microscopy. At the gene level, none of the investigated molecules were significantly altered in HS-NL skin, while 11 molecules changed significantly in HS-L skin versus control. At the protein level, the investigated molecules were similarly expressed in HS-NL and AGR skin. In HS-L skin, only slight changes were detected; however, differences did not show a unidirectional alteration, as KRT1 and KLK5 were detected in decreased levels, and KLK7, KRT6 and DSG1 in increased levels. No significant differences in TEWL or the expression of junction structures were assessed. Our findings suggest that the permeability barrier is not significantly damaged in HS skin and permeability barrier alterations are not the driver factors of keratinocyte activation in this disease.
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spelling pubmed-98556472023-01-21 New Data on the Features of Skin Barrier in Hidradenitis Suppurativa Somogyi, Orsolya Dajnoki, Zsolt Szabó, Lilla Gáspár, Krisztián Hendrik, Zoltán Zouboulis, Christos C. Dócs, Klaudia Szücs, Péter Dull, Katalin Törőcsik, Dániel Kapitány, Anikó Szegedi, Andrea Biomedicines Article Hidradenitis suppurativa (HS) is a Th1/17-driven inflammatory skin disease of the apocrine gland-rich (AGR) skin regions, where keratinocytes seem to be the crucial drivers of the initial pathogenic steps. However, the possible role of permeability barrier alteration in activating keratinocytes during HS development has not been clarified. We compared the major permeability barrier elements of non-lesional HS (HS-NL; n = 10) and lesional HS (HS-L; n = 10) skin with healthy AGR regions (n = 10) via RT-qPCR and immunohistochemistry. Stratum corneum components related to cornified envelope formation, corneocyte desquamation and (corneo)desmosome organization were analyzed along with tight junction molecules and barrier alarmins. The permeability barrier function was also investigated with transepidermal water loss (TEWL) measurements (n = 16). Junction structures were also visualized using confocal microscopy. At the gene level, none of the investigated molecules were significantly altered in HS-NL skin, while 11 molecules changed significantly in HS-L skin versus control. At the protein level, the investigated molecules were similarly expressed in HS-NL and AGR skin. In HS-L skin, only slight changes were detected; however, differences did not show a unidirectional alteration, as KRT1 and KLK5 were detected in decreased levels, and KLK7, KRT6 and DSG1 in increased levels. No significant differences in TEWL or the expression of junction structures were assessed. Our findings suggest that the permeability barrier is not significantly damaged in HS skin and permeability barrier alterations are not the driver factors of keratinocyte activation in this disease. MDPI 2023-01-04 /pmc/articles/PMC9855647/ /pubmed/36672635 http://dx.doi.org/10.3390/biomedicines11010127 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Somogyi, Orsolya
Dajnoki, Zsolt
Szabó, Lilla
Gáspár, Krisztián
Hendrik, Zoltán
Zouboulis, Christos C.
Dócs, Klaudia
Szücs, Péter
Dull, Katalin
Törőcsik, Dániel
Kapitány, Anikó
Szegedi, Andrea
New Data on the Features of Skin Barrier in Hidradenitis Suppurativa
title New Data on the Features of Skin Barrier in Hidradenitis Suppurativa
title_full New Data on the Features of Skin Barrier in Hidradenitis Suppurativa
title_fullStr New Data on the Features of Skin Barrier in Hidradenitis Suppurativa
title_full_unstemmed New Data on the Features of Skin Barrier in Hidradenitis Suppurativa
title_short New Data on the Features of Skin Barrier in Hidradenitis Suppurativa
title_sort new data on the features of skin barrier in hidradenitis suppurativa
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9855647/
https://www.ncbi.nlm.nih.gov/pubmed/36672635
http://dx.doi.org/10.3390/biomedicines11010127
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