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New Data on the Features of Skin Barrier in Hidradenitis Suppurativa
Hidradenitis suppurativa (HS) is a Th1/17-driven inflammatory skin disease of the apocrine gland-rich (AGR) skin regions, where keratinocytes seem to be the crucial drivers of the initial pathogenic steps. However, the possible role of permeability barrier alteration in activating keratinocytes duri...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9855647/ https://www.ncbi.nlm.nih.gov/pubmed/36672635 http://dx.doi.org/10.3390/biomedicines11010127 |
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author | Somogyi, Orsolya Dajnoki, Zsolt Szabó, Lilla Gáspár, Krisztián Hendrik, Zoltán Zouboulis, Christos C. Dócs, Klaudia Szücs, Péter Dull, Katalin Törőcsik, Dániel Kapitány, Anikó Szegedi, Andrea |
author_facet | Somogyi, Orsolya Dajnoki, Zsolt Szabó, Lilla Gáspár, Krisztián Hendrik, Zoltán Zouboulis, Christos C. Dócs, Klaudia Szücs, Péter Dull, Katalin Törőcsik, Dániel Kapitány, Anikó Szegedi, Andrea |
author_sort | Somogyi, Orsolya |
collection | PubMed |
description | Hidradenitis suppurativa (HS) is a Th1/17-driven inflammatory skin disease of the apocrine gland-rich (AGR) skin regions, where keratinocytes seem to be the crucial drivers of the initial pathogenic steps. However, the possible role of permeability barrier alteration in activating keratinocytes during HS development has not been clarified. We compared the major permeability barrier elements of non-lesional HS (HS-NL; n = 10) and lesional HS (HS-L; n = 10) skin with healthy AGR regions (n = 10) via RT-qPCR and immunohistochemistry. Stratum corneum components related to cornified envelope formation, corneocyte desquamation and (corneo)desmosome organization were analyzed along with tight junction molecules and barrier alarmins. The permeability barrier function was also investigated with transepidermal water loss (TEWL) measurements (n = 16). Junction structures were also visualized using confocal microscopy. At the gene level, none of the investigated molecules were significantly altered in HS-NL skin, while 11 molecules changed significantly in HS-L skin versus control. At the protein level, the investigated molecules were similarly expressed in HS-NL and AGR skin. In HS-L skin, only slight changes were detected; however, differences did not show a unidirectional alteration, as KRT1 and KLK5 were detected in decreased levels, and KLK7, KRT6 and DSG1 in increased levels. No significant differences in TEWL or the expression of junction structures were assessed. Our findings suggest that the permeability barrier is not significantly damaged in HS skin and permeability barrier alterations are not the driver factors of keratinocyte activation in this disease. |
format | Online Article Text |
id | pubmed-9855647 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-98556472023-01-21 New Data on the Features of Skin Barrier in Hidradenitis Suppurativa Somogyi, Orsolya Dajnoki, Zsolt Szabó, Lilla Gáspár, Krisztián Hendrik, Zoltán Zouboulis, Christos C. Dócs, Klaudia Szücs, Péter Dull, Katalin Törőcsik, Dániel Kapitány, Anikó Szegedi, Andrea Biomedicines Article Hidradenitis suppurativa (HS) is a Th1/17-driven inflammatory skin disease of the apocrine gland-rich (AGR) skin regions, where keratinocytes seem to be the crucial drivers of the initial pathogenic steps. However, the possible role of permeability barrier alteration in activating keratinocytes during HS development has not been clarified. We compared the major permeability barrier elements of non-lesional HS (HS-NL; n = 10) and lesional HS (HS-L; n = 10) skin with healthy AGR regions (n = 10) via RT-qPCR and immunohistochemistry. Stratum corneum components related to cornified envelope formation, corneocyte desquamation and (corneo)desmosome organization were analyzed along with tight junction molecules and barrier alarmins. The permeability barrier function was also investigated with transepidermal water loss (TEWL) measurements (n = 16). Junction structures were also visualized using confocal microscopy. At the gene level, none of the investigated molecules were significantly altered in HS-NL skin, while 11 molecules changed significantly in HS-L skin versus control. At the protein level, the investigated molecules were similarly expressed in HS-NL and AGR skin. In HS-L skin, only slight changes were detected; however, differences did not show a unidirectional alteration, as KRT1 and KLK5 were detected in decreased levels, and KLK7, KRT6 and DSG1 in increased levels. No significant differences in TEWL or the expression of junction structures were assessed. Our findings suggest that the permeability barrier is not significantly damaged in HS skin and permeability barrier alterations are not the driver factors of keratinocyte activation in this disease. MDPI 2023-01-04 /pmc/articles/PMC9855647/ /pubmed/36672635 http://dx.doi.org/10.3390/biomedicines11010127 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Somogyi, Orsolya Dajnoki, Zsolt Szabó, Lilla Gáspár, Krisztián Hendrik, Zoltán Zouboulis, Christos C. Dócs, Klaudia Szücs, Péter Dull, Katalin Törőcsik, Dániel Kapitány, Anikó Szegedi, Andrea New Data on the Features of Skin Barrier in Hidradenitis Suppurativa |
title | New Data on the Features of Skin Barrier in Hidradenitis Suppurativa |
title_full | New Data on the Features of Skin Barrier in Hidradenitis Suppurativa |
title_fullStr | New Data on the Features of Skin Barrier in Hidradenitis Suppurativa |
title_full_unstemmed | New Data on the Features of Skin Barrier in Hidradenitis Suppurativa |
title_short | New Data on the Features of Skin Barrier in Hidradenitis Suppurativa |
title_sort | new data on the features of skin barrier in hidradenitis suppurativa |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9855647/ https://www.ncbi.nlm.nih.gov/pubmed/36672635 http://dx.doi.org/10.3390/biomedicines11010127 |
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