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Electrochemical Aptasensor Developed Using Two-Electrode Setup and Three-Electrode Setup: Comprising Their Current Range in Context of Dengue Virus Determination

We present, for the very first time, the fabrication and electrochemical characterization of a paper-based experimental platform for dengue virus analysis. The paper-based device incorporates a screen-printing technology with the help of black carbon conductive ink. The paper-based device utilizes t...

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Detalles Bibliográficos
Autores principales: Hasan, Mohd. Rahil, Sharma, Pradakshina, Shaikh, Shifa, Singh, Saumitra, Pilloton, Roberto, Narang, Jagriti
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9855785/
https://www.ncbi.nlm.nih.gov/pubmed/36671836
http://dx.doi.org/10.3390/bios13010001
Descripción
Sumario:We present, for the very first time, the fabrication and electrochemical characterization of a paper-based experimental platform for dengue virus analysis. The paper-based device incorporates a screen-printing technology with the help of black carbon conductive ink. The paper-based device utilizes two styles of electrode setups, i.e., the two-electrode system and three-electrode system, and both setups effectively detected the dengue virus with an LOD of 0.1 µg/mL; however, these paper electrodes exhibit various current ranges, and the created sensor was encompassed and compared in this research based on current response. It is observed that the three-electrode system has a substantially higher current range, ranging from 55.53 µA to 322.21 µA, as compared to the two-electrode system, which has a current range of 0.85 µA to 4.54 µA. According to this study, the three-electrode system displayed a good range of current amplification that is roughly 50 times higher than the two-electrode system, which had a weak current response. As a result, the three-electrode method has emerged as a viable option for the very sensitive detection of the dengue virus, as well as for the diagnosis of other diseases.