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Electrochemical Aptasensor Developed Using Two-Electrode Setup and Three-Electrode Setup: Comprising Their Current Range in Context of Dengue Virus Determination

We present, for the very first time, the fabrication and electrochemical characterization of a paper-based experimental platform for dengue virus analysis. The paper-based device incorporates a screen-printing technology with the help of black carbon conductive ink. The paper-based device utilizes t...

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Autores principales: Hasan, Mohd. Rahil, Sharma, Pradakshina, Shaikh, Shifa, Singh, Saumitra, Pilloton, Roberto, Narang, Jagriti
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9855785/
https://www.ncbi.nlm.nih.gov/pubmed/36671836
http://dx.doi.org/10.3390/bios13010001
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author Hasan, Mohd. Rahil
Sharma, Pradakshina
Shaikh, Shifa
Singh, Saumitra
Pilloton, Roberto
Narang, Jagriti
author_facet Hasan, Mohd. Rahil
Sharma, Pradakshina
Shaikh, Shifa
Singh, Saumitra
Pilloton, Roberto
Narang, Jagriti
author_sort Hasan, Mohd. Rahil
collection PubMed
description We present, for the very first time, the fabrication and electrochemical characterization of a paper-based experimental platform for dengue virus analysis. The paper-based device incorporates a screen-printing technology with the help of black carbon conductive ink. The paper-based device utilizes two styles of electrode setups, i.e., the two-electrode system and three-electrode system, and both setups effectively detected the dengue virus with an LOD of 0.1 µg/mL; however, these paper electrodes exhibit various current ranges, and the created sensor was encompassed and compared in this research based on current response. It is observed that the three-electrode system has a substantially higher current range, ranging from 55.53 µA to 322.21 µA, as compared to the two-electrode system, which has a current range of 0.85 µA to 4.54 µA. According to this study, the three-electrode system displayed a good range of current amplification that is roughly 50 times higher than the two-electrode system, which had a weak current response. As a result, the three-electrode method has emerged as a viable option for the very sensitive detection of the dengue virus, as well as for the diagnosis of other diseases.
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spelling pubmed-98557852023-01-21 Electrochemical Aptasensor Developed Using Two-Electrode Setup and Three-Electrode Setup: Comprising Their Current Range in Context of Dengue Virus Determination Hasan, Mohd. Rahil Sharma, Pradakshina Shaikh, Shifa Singh, Saumitra Pilloton, Roberto Narang, Jagriti Biosensors (Basel) Communication We present, for the very first time, the fabrication and electrochemical characterization of a paper-based experimental platform for dengue virus analysis. The paper-based device incorporates a screen-printing technology with the help of black carbon conductive ink. The paper-based device utilizes two styles of electrode setups, i.e., the two-electrode system and three-electrode system, and both setups effectively detected the dengue virus with an LOD of 0.1 µg/mL; however, these paper electrodes exhibit various current ranges, and the created sensor was encompassed and compared in this research based on current response. It is observed that the three-electrode system has a substantially higher current range, ranging from 55.53 µA to 322.21 µA, as compared to the two-electrode system, which has a current range of 0.85 µA to 4.54 µA. According to this study, the three-electrode system displayed a good range of current amplification that is roughly 50 times higher than the two-electrode system, which had a weak current response. As a result, the three-electrode method has emerged as a viable option for the very sensitive detection of the dengue virus, as well as for the diagnosis of other diseases. MDPI 2022-12-20 /pmc/articles/PMC9855785/ /pubmed/36671836 http://dx.doi.org/10.3390/bios13010001 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Hasan, Mohd. Rahil
Sharma, Pradakshina
Shaikh, Shifa
Singh, Saumitra
Pilloton, Roberto
Narang, Jagriti
Electrochemical Aptasensor Developed Using Two-Electrode Setup and Three-Electrode Setup: Comprising Their Current Range in Context of Dengue Virus Determination
title Electrochemical Aptasensor Developed Using Two-Electrode Setup and Three-Electrode Setup: Comprising Their Current Range in Context of Dengue Virus Determination
title_full Electrochemical Aptasensor Developed Using Two-Electrode Setup and Three-Electrode Setup: Comprising Their Current Range in Context of Dengue Virus Determination
title_fullStr Electrochemical Aptasensor Developed Using Two-Electrode Setup and Three-Electrode Setup: Comprising Their Current Range in Context of Dengue Virus Determination
title_full_unstemmed Electrochemical Aptasensor Developed Using Two-Electrode Setup and Three-Electrode Setup: Comprising Their Current Range in Context of Dengue Virus Determination
title_short Electrochemical Aptasensor Developed Using Two-Electrode Setup and Three-Electrode Setup: Comprising Their Current Range in Context of Dengue Virus Determination
title_sort electrochemical aptasensor developed using two-electrode setup and three-electrode setup: comprising their current range in context of dengue virus determination
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9855785/
https://www.ncbi.nlm.nih.gov/pubmed/36671836
http://dx.doi.org/10.3390/bios13010001
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