Cargando…

Biofunctionalization of Multiplexed Silicon Photonic Biosensors

Silicon photonic (SiP) sensors offer a promising platform for robust and low-cost decentralized diagnostics due to their high scalability, low limit of detection, and ability to integrate multiple sensors for multiplexed analyte detection. Their CMOS-compatible fabrication enables chip-scale miniatu...

Descripción completa

Detalles Bibliográficos
Autores principales: Puumala, Lauren S., Grist, Samantha M., Morales, Jennifer M., Bickford, Justin R., Chrostowski, Lukas, Shekhar, Sudip, Cheung, Karen C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9855810/
https://www.ncbi.nlm.nih.gov/pubmed/36671887
http://dx.doi.org/10.3390/bios13010053
_version_ 1784873466768392192
author Puumala, Lauren S.
Grist, Samantha M.
Morales, Jennifer M.
Bickford, Justin R.
Chrostowski, Lukas
Shekhar, Sudip
Cheung, Karen C.
author_facet Puumala, Lauren S.
Grist, Samantha M.
Morales, Jennifer M.
Bickford, Justin R.
Chrostowski, Lukas
Shekhar, Sudip
Cheung, Karen C.
author_sort Puumala, Lauren S.
collection PubMed
description Silicon photonic (SiP) sensors offer a promising platform for robust and low-cost decentralized diagnostics due to their high scalability, low limit of detection, and ability to integrate multiple sensors for multiplexed analyte detection. Their CMOS-compatible fabrication enables chip-scale miniaturization, high scalability, and low-cost mass production. Sensitive, specific detection with silicon photonic sensors is afforded through biofunctionalization of the sensor surface; consequently, this functionalization chemistry is inextricably linked to sensor performance. In this review, we first highlight the biofunctionalization needs for SiP biosensors, including sensitivity, specificity, cost, shelf-stability, and replicability and establish a set of performance criteria. We then benchmark biofunctionalization strategies for SiP biosensors against these criteria, organizing the review around three key aspects: bioreceptor selection, immobilization strategies, and patterning techniques. First, we evaluate bioreceptors, including antibodies, aptamers, nucleic acid probes, molecularly imprinted polymers, peptides, glycans, and lectins. We then compare adsorption, bioaffinity, and covalent chemistries for immobilizing bioreceptors on SiP surfaces. Finally, we compare biopatterning techniques for spatially controlling and multiplexing the biofunctionalization of SiP sensors, including microcontact printing, pin- and pipette-based spotting, microfluidic patterning in channels, inkjet printing, and microfluidic probes.
format Online
Article
Text
id pubmed-9855810
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-98558102023-01-21 Biofunctionalization of Multiplexed Silicon Photonic Biosensors Puumala, Lauren S. Grist, Samantha M. Morales, Jennifer M. Bickford, Justin R. Chrostowski, Lukas Shekhar, Sudip Cheung, Karen C. Biosensors (Basel) Review Silicon photonic (SiP) sensors offer a promising platform for robust and low-cost decentralized diagnostics due to their high scalability, low limit of detection, and ability to integrate multiple sensors for multiplexed analyte detection. Their CMOS-compatible fabrication enables chip-scale miniaturization, high scalability, and low-cost mass production. Sensitive, specific detection with silicon photonic sensors is afforded through biofunctionalization of the sensor surface; consequently, this functionalization chemistry is inextricably linked to sensor performance. In this review, we first highlight the biofunctionalization needs for SiP biosensors, including sensitivity, specificity, cost, shelf-stability, and replicability and establish a set of performance criteria. We then benchmark biofunctionalization strategies for SiP biosensors against these criteria, organizing the review around three key aspects: bioreceptor selection, immobilization strategies, and patterning techniques. First, we evaluate bioreceptors, including antibodies, aptamers, nucleic acid probes, molecularly imprinted polymers, peptides, glycans, and lectins. We then compare adsorption, bioaffinity, and covalent chemistries for immobilizing bioreceptors on SiP surfaces. Finally, we compare biopatterning techniques for spatially controlling and multiplexing the biofunctionalization of SiP sensors, including microcontact printing, pin- and pipette-based spotting, microfluidic patterning in channels, inkjet printing, and microfluidic probes. MDPI 2022-12-29 /pmc/articles/PMC9855810/ /pubmed/36671887 http://dx.doi.org/10.3390/bios13010053 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Puumala, Lauren S.
Grist, Samantha M.
Morales, Jennifer M.
Bickford, Justin R.
Chrostowski, Lukas
Shekhar, Sudip
Cheung, Karen C.
Biofunctionalization of Multiplexed Silicon Photonic Biosensors
title Biofunctionalization of Multiplexed Silicon Photonic Biosensors
title_full Biofunctionalization of Multiplexed Silicon Photonic Biosensors
title_fullStr Biofunctionalization of Multiplexed Silicon Photonic Biosensors
title_full_unstemmed Biofunctionalization of Multiplexed Silicon Photonic Biosensors
title_short Biofunctionalization of Multiplexed Silicon Photonic Biosensors
title_sort biofunctionalization of multiplexed silicon photonic biosensors
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9855810/
https://www.ncbi.nlm.nih.gov/pubmed/36671887
http://dx.doi.org/10.3390/bios13010053
work_keys_str_mv AT puumalalaurens biofunctionalizationofmultiplexedsiliconphotonicbiosensors
AT gristsamantham biofunctionalizationofmultiplexedsiliconphotonicbiosensors
AT moralesjenniferm biofunctionalizationofmultiplexedsiliconphotonicbiosensors
AT bickfordjustinr biofunctionalizationofmultiplexedsiliconphotonicbiosensors
AT chrostowskilukas biofunctionalizationofmultiplexedsiliconphotonicbiosensors
AT shekharsudip biofunctionalizationofmultiplexedsiliconphotonicbiosensors
AT cheungkarenc biofunctionalizationofmultiplexedsiliconphotonicbiosensors