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Biofunctionalization of Multiplexed Silicon Photonic Biosensors
Silicon photonic (SiP) sensors offer a promising platform for robust and low-cost decentralized diagnostics due to their high scalability, low limit of detection, and ability to integrate multiple sensors for multiplexed analyte detection. Their CMOS-compatible fabrication enables chip-scale miniatu...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9855810/ https://www.ncbi.nlm.nih.gov/pubmed/36671887 http://dx.doi.org/10.3390/bios13010053 |
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author | Puumala, Lauren S. Grist, Samantha M. Morales, Jennifer M. Bickford, Justin R. Chrostowski, Lukas Shekhar, Sudip Cheung, Karen C. |
author_facet | Puumala, Lauren S. Grist, Samantha M. Morales, Jennifer M. Bickford, Justin R. Chrostowski, Lukas Shekhar, Sudip Cheung, Karen C. |
author_sort | Puumala, Lauren S. |
collection | PubMed |
description | Silicon photonic (SiP) sensors offer a promising platform for robust and low-cost decentralized diagnostics due to their high scalability, low limit of detection, and ability to integrate multiple sensors for multiplexed analyte detection. Their CMOS-compatible fabrication enables chip-scale miniaturization, high scalability, and low-cost mass production. Sensitive, specific detection with silicon photonic sensors is afforded through biofunctionalization of the sensor surface; consequently, this functionalization chemistry is inextricably linked to sensor performance. In this review, we first highlight the biofunctionalization needs for SiP biosensors, including sensitivity, specificity, cost, shelf-stability, and replicability and establish a set of performance criteria. We then benchmark biofunctionalization strategies for SiP biosensors against these criteria, organizing the review around three key aspects: bioreceptor selection, immobilization strategies, and patterning techniques. First, we evaluate bioreceptors, including antibodies, aptamers, nucleic acid probes, molecularly imprinted polymers, peptides, glycans, and lectins. We then compare adsorption, bioaffinity, and covalent chemistries for immobilizing bioreceptors on SiP surfaces. Finally, we compare biopatterning techniques for spatially controlling and multiplexing the biofunctionalization of SiP sensors, including microcontact printing, pin- and pipette-based spotting, microfluidic patterning in channels, inkjet printing, and microfluidic probes. |
format | Online Article Text |
id | pubmed-9855810 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-98558102023-01-21 Biofunctionalization of Multiplexed Silicon Photonic Biosensors Puumala, Lauren S. Grist, Samantha M. Morales, Jennifer M. Bickford, Justin R. Chrostowski, Lukas Shekhar, Sudip Cheung, Karen C. Biosensors (Basel) Review Silicon photonic (SiP) sensors offer a promising platform for robust and low-cost decentralized diagnostics due to their high scalability, low limit of detection, and ability to integrate multiple sensors for multiplexed analyte detection. Their CMOS-compatible fabrication enables chip-scale miniaturization, high scalability, and low-cost mass production. Sensitive, specific detection with silicon photonic sensors is afforded through biofunctionalization of the sensor surface; consequently, this functionalization chemistry is inextricably linked to sensor performance. In this review, we first highlight the biofunctionalization needs for SiP biosensors, including sensitivity, specificity, cost, shelf-stability, and replicability and establish a set of performance criteria. We then benchmark biofunctionalization strategies for SiP biosensors against these criteria, organizing the review around three key aspects: bioreceptor selection, immobilization strategies, and patterning techniques. First, we evaluate bioreceptors, including antibodies, aptamers, nucleic acid probes, molecularly imprinted polymers, peptides, glycans, and lectins. We then compare adsorption, bioaffinity, and covalent chemistries for immobilizing bioreceptors on SiP surfaces. Finally, we compare biopatterning techniques for spatially controlling and multiplexing the biofunctionalization of SiP sensors, including microcontact printing, pin- and pipette-based spotting, microfluidic patterning in channels, inkjet printing, and microfluidic probes. MDPI 2022-12-29 /pmc/articles/PMC9855810/ /pubmed/36671887 http://dx.doi.org/10.3390/bios13010053 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Puumala, Lauren S. Grist, Samantha M. Morales, Jennifer M. Bickford, Justin R. Chrostowski, Lukas Shekhar, Sudip Cheung, Karen C. Biofunctionalization of Multiplexed Silicon Photonic Biosensors |
title | Biofunctionalization of Multiplexed Silicon Photonic Biosensors |
title_full | Biofunctionalization of Multiplexed Silicon Photonic Biosensors |
title_fullStr | Biofunctionalization of Multiplexed Silicon Photonic Biosensors |
title_full_unstemmed | Biofunctionalization of Multiplexed Silicon Photonic Biosensors |
title_short | Biofunctionalization of Multiplexed Silicon Photonic Biosensors |
title_sort | biofunctionalization of multiplexed silicon photonic biosensors |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9855810/ https://www.ncbi.nlm.nih.gov/pubmed/36671887 http://dx.doi.org/10.3390/bios13010053 |
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