Peptide Nanosheet-Inspired Biomimetic Synthesis of CuS Nanoparticles on Ti(3)C(2) Nanosheets for Electrochemical Biosensing of Hydrogen Peroxide

Hydrogen peroxide (H(2)O(2)) is one of the intermediates or final products of biological metabolism and participates in many important biological processes of life activities. The detection of H(2)O(2) is of great significance in clinical disease monitoring, environmental protection, and bioanalysis. In this study, Ti(3)C(2)-based nanohybrids are prepared by the biological modification and self-assembled peptide nanosheets (PNSs)-based biomimetic synthesis of copper sulfide nanoparticles (CuS NPs), which show potential application in the fabrication of low-cost and high-performance electrochemical H(2)O(2) biosensors. The synthesized CuS-PNSs/Ti(3)C(2) nanohybrids exhibit excellent electrochemical performance towards H(2)O(2), in which CuS NPs can catalyze the decomposition of H(2)O(2) and realize the transformation from a chemical signal to an electrical signal to achieve the purpose of H(2)O(2) detection. The prepared CuS-PNSs/Ti(3)C(2)-based electrochemical biosensor platform exhibits a wide detection range (5 μM–15 mM) and a low detection limit (0.226 μM). In addition, it reveals good selectivity and stability and can realize the monitoring of H(2)O(2) in a complex environment. The successful biomimetic synthesis of CuS-PNSs/Ti(3)C(2) hybrid nanomaterials provides a green and friendly strategy for the design and synthesis of functional nanomaterials and also provides a new inspiration for the construction of highly effective electrochemical biosensors for practical detection of H(2)O(2) in various environments.