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Necroptosis Related Genes Predict Prognosis and Therapeutic Potential in Gastric Cancer

The clinical significance of necroptosis in gastric cancer (GC) has yet to be fully elucidated. The purpose of our study was to identify a necroptosis-relevant gene and to establish a prediction model to estimate the prognosis and therapeutic potential in GC. Here, we explored the expression profile...

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Autores principales: Wu, Nandie, Liu, Fangcen, Huang, Ying, Su, Xinyu, Zhang, Yaping, Yu, Lixia, Liu, Baorui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9856014/
https://www.ncbi.nlm.nih.gov/pubmed/36671486
http://dx.doi.org/10.3390/biom13010101
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author Wu, Nandie
Liu, Fangcen
Huang, Ying
Su, Xinyu
Zhang, Yaping
Yu, Lixia
Liu, Baorui
author_facet Wu, Nandie
Liu, Fangcen
Huang, Ying
Su, Xinyu
Zhang, Yaping
Yu, Lixia
Liu, Baorui
author_sort Wu, Nandie
collection PubMed
description The clinical significance of necroptosis in gastric cancer (GC) has yet to be fully elucidated. The purpose of our study was to identify a necroptosis-relevant gene and to establish a prediction model to estimate the prognosis and therapeutic potential in GC. Here, we explored the expression profile of 76 necroptosis-related genes in TCGA-STAD patients. A six-gene risk score prediction model was established via regression analysis of the least absolute shrinkage and selection operator (LASSO) and validated in a separate cohort. Patients were separated into low- or high-risk groups according to the median risk score. We then compared and analyzed the biological process characteristics of two risk groups. Additionally, cell-to-cell communications and metabolic activity were analyzed in a single-cell solution. The in vitro experiments were conducted to explore the biological functions and drug sensitivity of necroptosis-related genes in gastric cancer. Our results identified that compared with the low-risk group, the high-risk group was associated with a higher clinical stage or grade and a worse prognosis. In addition, the low-risk group had higher levels of immunity and immune cell infiltration. Necroptosis was triggered by the TNF pathway in myeloid cells and the glycolysis pathway was altered. Necroptosis-related genes modulated the cell function, including proliferation and migration in vitro. Furthermore, the potential drugs’ sensitivity was higher in the low-risk subgroup. These findings could facilitate a better understanding and improve the treatment potential and prognosis of GC patients.
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spelling pubmed-98560142023-01-21 Necroptosis Related Genes Predict Prognosis and Therapeutic Potential in Gastric Cancer Wu, Nandie Liu, Fangcen Huang, Ying Su, Xinyu Zhang, Yaping Yu, Lixia Liu, Baorui Biomolecules Article The clinical significance of necroptosis in gastric cancer (GC) has yet to be fully elucidated. The purpose of our study was to identify a necroptosis-relevant gene and to establish a prediction model to estimate the prognosis and therapeutic potential in GC. Here, we explored the expression profile of 76 necroptosis-related genes in TCGA-STAD patients. A six-gene risk score prediction model was established via regression analysis of the least absolute shrinkage and selection operator (LASSO) and validated in a separate cohort. Patients were separated into low- or high-risk groups according to the median risk score. We then compared and analyzed the biological process characteristics of two risk groups. Additionally, cell-to-cell communications and metabolic activity were analyzed in a single-cell solution. The in vitro experiments were conducted to explore the biological functions and drug sensitivity of necroptosis-related genes in gastric cancer. Our results identified that compared with the low-risk group, the high-risk group was associated with a higher clinical stage or grade and a worse prognosis. In addition, the low-risk group had higher levels of immunity and immune cell infiltration. Necroptosis was triggered by the TNF pathway in myeloid cells and the glycolysis pathway was altered. Necroptosis-related genes modulated the cell function, including proliferation and migration in vitro. Furthermore, the potential drugs’ sensitivity was higher in the low-risk subgroup. These findings could facilitate a better understanding and improve the treatment potential and prognosis of GC patients. MDPI 2023-01-04 /pmc/articles/PMC9856014/ /pubmed/36671486 http://dx.doi.org/10.3390/biom13010101 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wu, Nandie
Liu, Fangcen
Huang, Ying
Su, Xinyu
Zhang, Yaping
Yu, Lixia
Liu, Baorui
Necroptosis Related Genes Predict Prognosis and Therapeutic Potential in Gastric Cancer
title Necroptosis Related Genes Predict Prognosis and Therapeutic Potential in Gastric Cancer
title_full Necroptosis Related Genes Predict Prognosis and Therapeutic Potential in Gastric Cancer
title_fullStr Necroptosis Related Genes Predict Prognosis and Therapeutic Potential in Gastric Cancer
title_full_unstemmed Necroptosis Related Genes Predict Prognosis and Therapeutic Potential in Gastric Cancer
title_short Necroptosis Related Genes Predict Prognosis and Therapeutic Potential in Gastric Cancer
title_sort necroptosis related genes predict prognosis and therapeutic potential in gastric cancer
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9856014/
https://www.ncbi.nlm.nih.gov/pubmed/36671486
http://dx.doi.org/10.3390/biom13010101
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