HIF1A Knockout by Biallelic and Selection-Free CRISPR Gene Editing in Human Primary Endothelial Cells with Ribonucleoprotein Complexes

Primary endothelial cells (ECs), especially human umbilical vein endothelial cells (HUVECs), are broadly used in vascular biology. Gene editing of primary endothelial cells is known to be challenging, due to the low DNA transfection efficiency and the limited proliferation capacity of ECs. We report...

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Detalles Bibliográficos
Autores principales: Brandt, Camilla Blunk, Fonager, Sofie Vestergaard, Haskó, János, Helmig, Rikke Bek, Degn, Søren, Bolund, Lars, Jessen, Niels, Lin, Lin, Luo, Yonglun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9856017/
https://www.ncbi.nlm.nih.gov/pubmed/36671408
http://dx.doi.org/10.3390/biom13010023

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