Cargando…
An In Vivo Screening Model for Investigation of Pathophysiology of Human Implantation Failure
To improve current infertility treatments, it is important to understand the pathophysiology of implantation failure. However, many molecules are involved in the normal biological process of implantation and the roles of each molecule and the molecular mechanism are not fully understood. This review...
Autores principales: | , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9856033/ https://www.ncbi.nlm.nih.gov/pubmed/36671464 http://dx.doi.org/10.3390/biom13010079 |
_version_ | 1784873522178293760 |
---|---|
author | Nakamura, Hitomi Kimura, Tadashi |
author_facet | Nakamura, Hitomi Kimura, Tadashi |
author_sort | Nakamura, Hitomi |
collection | PubMed |
description | To improve current infertility treatments, it is important to understand the pathophysiology of implantation failure. However, many molecules are involved in the normal biological process of implantation and the roles of each molecule and the molecular mechanism are not fully understood. This review highlights the hemagglutinating virus of Japan (HVJ; Sendai virus) envelope (HVJ-E) vector, which uses inactivated viral particles as a local and transient gene transfer system to the murine uterus during the implantation period in order to investigate the molecular mechanism of implantation. In vivo screening in mice using the HVJ-E vector system suggests that signal transducer and activator of transcription-3 (Stat-3) could be a diagnostic and therapeutic target for women with a history of implantation failure. The HVJ-E vector system hardly induces complete defects in genes; however, it not only suppresses but also transiently overexpresses some genes in the murine uterus. These features may be useful in investigating the pathophysiology of implantation failure in women. |
format | Online Article Text |
id | pubmed-9856033 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-98560332023-01-21 An In Vivo Screening Model for Investigation of Pathophysiology of Human Implantation Failure Nakamura, Hitomi Kimura, Tadashi Biomolecules Review To improve current infertility treatments, it is important to understand the pathophysiology of implantation failure. However, many molecules are involved in the normal biological process of implantation and the roles of each molecule and the molecular mechanism are not fully understood. This review highlights the hemagglutinating virus of Japan (HVJ; Sendai virus) envelope (HVJ-E) vector, which uses inactivated viral particles as a local and transient gene transfer system to the murine uterus during the implantation period in order to investigate the molecular mechanism of implantation. In vivo screening in mice using the HVJ-E vector system suggests that signal transducer and activator of transcription-3 (Stat-3) could be a diagnostic and therapeutic target for women with a history of implantation failure. The HVJ-E vector system hardly induces complete defects in genes; however, it not only suppresses but also transiently overexpresses some genes in the murine uterus. These features may be useful in investigating the pathophysiology of implantation failure in women. MDPI 2022-12-30 /pmc/articles/PMC9856033/ /pubmed/36671464 http://dx.doi.org/10.3390/biom13010079 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Nakamura, Hitomi Kimura, Tadashi An In Vivo Screening Model for Investigation of Pathophysiology of Human Implantation Failure |
title | An In Vivo Screening Model for Investigation of Pathophysiology of Human Implantation Failure |
title_full | An In Vivo Screening Model for Investigation of Pathophysiology of Human Implantation Failure |
title_fullStr | An In Vivo Screening Model for Investigation of Pathophysiology of Human Implantation Failure |
title_full_unstemmed | An In Vivo Screening Model for Investigation of Pathophysiology of Human Implantation Failure |
title_short | An In Vivo Screening Model for Investigation of Pathophysiology of Human Implantation Failure |
title_sort | in vivo screening model for investigation of pathophysiology of human implantation failure |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9856033/ https://www.ncbi.nlm.nih.gov/pubmed/36671464 http://dx.doi.org/10.3390/biom13010079 |
work_keys_str_mv | AT nakamurahitomi aninvivoscreeningmodelforinvestigationofpathophysiologyofhumanimplantationfailure AT kimuratadashi aninvivoscreeningmodelforinvestigationofpathophysiologyofhumanimplantationfailure AT nakamurahitomi invivoscreeningmodelforinvestigationofpathophysiologyofhumanimplantationfailure AT kimuratadashi invivoscreeningmodelforinvestigationofpathophysiologyofhumanimplantationfailure |