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Antimicrobial Spectrum of Activity and Mechanism of Action of Linear Alpha-Helical Peptides Inspired by Shrimp Anti-Lipopolysaccharide Factors

Shrimp antilipopolysaccharide factors (ALFs) form a multifunctional and diverse family of antimicrobial host defense peptides (AMPs) composed of seven members (groups A to G), which differ in terms of their primary structure and biochemical properties. They are amphipathic peptides with two conserve...

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Autores principales: Matos, Gabriel Machado, Garcia-Teodoro, Beatriz, Martins, Camila Pimentel, Schmitt, Paulina, Guzmán, Fanny, de Freitas, Ana Claudia Oliveira, Stoco, Patricia Hermes, Ferreira, Fabienne Antunes, Stadnik, Marciel João, Robl, Diogo, Perazzolo, Luciane Maria, Rosa, Rafael Diego
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9856130/
https://www.ncbi.nlm.nih.gov/pubmed/36671535
http://dx.doi.org/10.3390/biom13010150
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author Matos, Gabriel Machado
Garcia-Teodoro, Beatriz
Martins, Camila Pimentel
Schmitt, Paulina
Guzmán, Fanny
de Freitas, Ana Claudia Oliveira
Stoco, Patricia Hermes
Ferreira, Fabienne Antunes
Stadnik, Marciel João
Robl, Diogo
Perazzolo, Luciane Maria
Rosa, Rafael Diego
author_facet Matos, Gabriel Machado
Garcia-Teodoro, Beatriz
Martins, Camila Pimentel
Schmitt, Paulina
Guzmán, Fanny
de Freitas, Ana Claudia Oliveira
Stoco, Patricia Hermes
Ferreira, Fabienne Antunes
Stadnik, Marciel João
Robl, Diogo
Perazzolo, Luciane Maria
Rosa, Rafael Diego
author_sort Matos, Gabriel Machado
collection PubMed
description Shrimp antilipopolysaccharide factors (ALFs) form a multifunctional and diverse family of antimicrobial host defense peptides (AMPs) composed of seven members (groups A to G), which differ in terms of their primary structure and biochemical properties. They are amphipathic peptides with two conserved cysteine residues stabilizing a central β-hairpin that is understood to be the core region for their biological activities. In this study, we synthetized three linear (cysteine-free) peptides based on the amino acid sequence of the central β-hairpin of the newly identified shrimp (Litopenaeus vannamei) ALFs from groups E to G. Unlike whole mature ALFs, the ALF-derived peptides exhibited an α-helix secondary structure. In vitro assays revealed that the synthetic peptides display a broad spectrum of activity against both Gram-positive and Gram-negative bacteria and fungi but not against the protozoan parasites Trypanosoma cruzi and Leishmania (L.) infantum. Remarkably, they displayed synergistic effects and showed the ability to permeabilize bacterial membranes, a mechanism of action of classical AMPs. Having shown low cytotoxicity to THP-1 human cells and being active against clinical multiresistant bacterial isolates, these nature-inspired peptides represent an interesting class of bioactive molecules with biotechnological potential for the development of novel therapeutics in medical sciences.
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spelling pubmed-98561302023-01-21 Antimicrobial Spectrum of Activity and Mechanism of Action of Linear Alpha-Helical Peptides Inspired by Shrimp Anti-Lipopolysaccharide Factors Matos, Gabriel Machado Garcia-Teodoro, Beatriz Martins, Camila Pimentel Schmitt, Paulina Guzmán, Fanny de Freitas, Ana Claudia Oliveira Stoco, Patricia Hermes Ferreira, Fabienne Antunes Stadnik, Marciel João Robl, Diogo Perazzolo, Luciane Maria Rosa, Rafael Diego Biomolecules Article Shrimp antilipopolysaccharide factors (ALFs) form a multifunctional and diverse family of antimicrobial host defense peptides (AMPs) composed of seven members (groups A to G), which differ in terms of their primary structure and biochemical properties. They are amphipathic peptides with two conserved cysteine residues stabilizing a central β-hairpin that is understood to be the core region for their biological activities. In this study, we synthetized three linear (cysteine-free) peptides based on the amino acid sequence of the central β-hairpin of the newly identified shrimp (Litopenaeus vannamei) ALFs from groups E to G. Unlike whole mature ALFs, the ALF-derived peptides exhibited an α-helix secondary structure. In vitro assays revealed that the synthetic peptides display a broad spectrum of activity against both Gram-positive and Gram-negative bacteria and fungi but not against the protozoan parasites Trypanosoma cruzi and Leishmania (L.) infantum. Remarkably, they displayed synergistic effects and showed the ability to permeabilize bacterial membranes, a mechanism of action of classical AMPs. Having shown low cytotoxicity to THP-1 human cells and being active against clinical multiresistant bacterial isolates, these nature-inspired peptides represent an interesting class of bioactive molecules with biotechnological potential for the development of novel therapeutics in medical sciences. MDPI 2023-01-11 /pmc/articles/PMC9856130/ /pubmed/36671535 http://dx.doi.org/10.3390/biom13010150 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Matos, Gabriel Machado
Garcia-Teodoro, Beatriz
Martins, Camila Pimentel
Schmitt, Paulina
Guzmán, Fanny
de Freitas, Ana Claudia Oliveira
Stoco, Patricia Hermes
Ferreira, Fabienne Antunes
Stadnik, Marciel João
Robl, Diogo
Perazzolo, Luciane Maria
Rosa, Rafael Diego
Antimicrobial Spectrum of Activity and Mechanism of Action of Linear Alpha-Helical Peptides Inspired by Shrimp Anti-Lipopolysaccharide Factors
title Antimicrobial Spectrum of Activity and Mechanism of Action of Linear Alpha-Helical Peptides Inspired by Shrimp Anti-Lipopolysaccharide Factors
title_full Antimicrobial Spectrum of Activity and Mechanism of Action of Linear Alpha-Helical Peptides Inspired by Shrimp Anti-Lipopolysaccharide Factors
title_fullStr Antimicrobial Spectrum of Activity and Mechanism of Action of Linear Alpha-Helical Peptides Inspired by Shrimp Anti-Lipopolysaccharide Factors
title_full_unstemmed Antimicrobial Spectrum of Activity and Mechanism of Action of Linear Alpha-Helical Peptides Inspired by Shrimp Anti-Lipopolysaccharide Factors
title_short Antimicrobial Spectrum of Activity and Mechanism of Action of Linear Alpha-Helical Peptides Inspired by Shrimp Anti-Lipopolysaccharide Factors
title_sort antimicrobial spectrum of activity and mechanism of action of linear alpha-helical peptides inspired by shrimp anti-lipopolysaccharide factors
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9856130/
https://www.ncbi.nlm.nih.gov/pubmed/36671535
http://dx.doi.org/10.3390/biom13010150
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