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Group I Metabotropic Glutamate Receptors Modulate Motility and Enteric Neural Activity in the Mouse Colon
Glutamate is the major excitatory neurotransmitter in the central nervous system, and there is evidence that Group-I metabotropic glutamate receptors (mGlu1 and mGlu5) have established roles in excitatory neurotransmission and synaptic plasticity. While glutamate is abundantly present in the gut, it...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9856182/ https://www.ncbi.nlm.nih.gov/pubmed/36671524 http://dx.doi.org/10.3390/biom13010139 |
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author | Leembruggen, Anita J. L. Lu, Yuqing Wang, Haozhe Uzungil, Volkan Renoir, Thibault Hannan, Anthony J. Stamp, Lincon A. Hao, Marlene M. Bornstein, Joel C. |
author_facet | Leembruggen, Anita J. L. Lu, Yuqing Wang, Haozhe Uzungil, Volkan Renoir, Thibault Hannan, Anthony J. Stamp, Lincon A. Hao, Marlene M. Bornstein, Joel C. |
author_sort | Leembruggen, Anita J. L. |
collection | PubMed |
description | Glutamate is the major excitatory neurotransmitter in the central nervous system, and there is evidence that Group-I metabotropic glutamate receptors (mGlu1 and mGlu5) have established roles in excitatory neurotransmission and synaptic plasticity. While glutamate is abundantly present in the gut, it plays a smaller role in neurotransmission in the enteric nervous system. In this study, we examined the roles of Group-I mGlu receptors in gastrointestinal function. We investigated the expression of Grm1 (mGlu1) and Grm5 (mGlu5) in the mouse myenteric plexus using RNAscope in situ hybridization. Live calcium imaging and motility analysis were performed on ex vivo preparations of the mouse colon. mGlu5 was found to play a role in excitatory enteric neurotransmission, as electrically-evoked calcium transients were sensitive to the mGlu5 antagonist MPEP. However, inhibition of mGlu5 activity did not affect colonic motor complexes (CMCs). Instead, inhibition of mGlu1 using BAY 36-7620 reduced CMC frequency but did not affect enteric neurotransmission. These data highlight complex roles for Group-I mGlu receptors in myenteric neuron activity and colonic function. |
format | Online Article Text |
id | pubmed-9856182 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-98561822023-01-21 Group I Metabotropic Glutamate Receptors Modulate Motility and Enteric Neural Activity in the Mouse Colon Leembruggen, Anita J. L. Lu, Yuqing Wang, Haozhe Uzungil, Volkan Renoir, Thibault Hannan, Anthony J. Stamp, Lincon A. Hao, Marlene M. Bornstein, Joel C. Biomolecules Article Glutamate is the major excitatory neurotransmitter in the central nervous system, and there is evidence that Group-I metabotropic glutamate receptors (mGlu1 and mGlu5) have established roles in excitatory neurotransmission and synaptic plasticity. While glutamate is abundantly present in the gut, it plays a smaller role in neurotransmission in the enteric nervous system. In this study, we examined the roles of Group-I mGlu receptors in gastrointestinal function. We investigated the expression of Grm1 (mGlu1) and Grm5 (mGlu5) in the mouse myenteric plexus using RNAscope in situ hybridization. Live calcium imaging and motility analysis were performed on ex vivo preparations of the mouse colon. mGlu5 was found to play a role in excitatory enteric neurotransmission, as electrically-evoked calcium transients were sensitive to the mGlu5 antagonist MPEP. However, inhibition of mGlu5 activity did not affect colonic motor complexes (CMCs). Instead, inhibition of mGlu1 using BAY 36-7620 reduced CMC frequency but did not affect enteric neurotransmission. These data highlight complex roles for Group-I mGlu receptors in myenteric neuron activity and colonic function. MDPI 2023-01-09 /pmc/articles/PMC9856182/ /pubmed/36671524 http://dx.doi.org/10.3390/biom13010139 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Leembruggen, Anita J. L. Lu, Yuqing Wang, Haozhe Uzungil, Volkan Renoir, Thibault Hannan, Anthony J. Stamp, Lincon A. Hao, Marlene M. Bornstein, Joel C. Group I Metabotropic Glutamate Receptors Modulate Motility and Enteric Neural Activity in the Mouse Colon |
title | Group I Metabotropic Glutamate Receptors Modulate Motility and Enteric Neural Activity in the Mouse Colon |
title_full | Group I Metabotropic Glutamate Receptors Modulate Motility and Enteric Neural Activity in the Mouse Colon |
title_fullStr | Group I Metabotropic Glutamate Receptors Modulate Motility and Enteric Neural Activity in the Mouse Colon |
title_full_unstemmed | Group I Metabotropic Glutamate Receptors Modulate Motility and Enteric Neural Activity in the Mouse Colon |
title_short | Group I Metabotropic Glutamate Receptors Modulate Motility and Enteric Neural Activity in the Mouse Colon |
title_sort | group i metabotropic glutamate receptors modulate motility and enteric neural activity in the mouse colon |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9856182/ https://www.ncbi.nlm.nih.gov/pubmed/36671524 http://dx.doi.org/10.3390/biom13010139 |
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