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Urinary Proteome Changes during Pregnancy in Rats

Pregnancy involves a significant number of physiological changes. A normal pregnancy is essential to ensure healthy maternal and fetal development. We sought to explore whether the urinary proteome could reflect the pregnancy process. Urine samples were collected from pregnant and control rats on va...

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Autores principales: Tang, Shuxuan, Gao, Youhe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9856192/
https://www.ncbi.nlm.nih.gov/pubmed/36671419
http://dx.doi.org/10.3390/biom13010034
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author Tang, Shuxuan
Gao, Youhe
author_facet Tang, Shuxuan
Gao, Youhe
author_sort Tang, Shuxuan
collection PubMed
description Pregnancy involves a significant number of physiological changes. A normal pregnancy is essential to ensure healthy maternal and fetal development. We sought to explore whether the urinary proteome could reflect the pregnancy process. Urine samples were collected from pregnant and control rats on various gestational days. The urinary proteome was profiled by liquid chromatography coupled with tandem mass spectrometry (LC–MS/MS), and differential proteins were obtained by comparing to the gestational day 1 of the same group at each time point. Many pathways related to embryo implantation and trophoblast differentiation were enriched in the early days in urine. Liver, kidney, and bone development started early to be enriched in the pregnant group, but not in the control group. Interestingly, the developmental processes of the fetal heart such as heart looping and endocardial cushion formation could be seen in urine of pregnant rats. Moreover, the timings were consistent with those of embryological studies. The timing of the surfactant appearance in urine was right before birth. The differential proteins related to pancreas development appeared in urine at the time during reported time of pancreatic cell proliferation and differentiation. These processes were enriched only in the pregnant group and not in the control group. Furthermore, coagulation-associated pathways were found to be increasingly prominent before labor. Our results indicated that the urine proteome of pregnant rats can reflect the process of pregnancy, even fetal embryonic development. Maternal urinary proteome detection was earlier than the developmental time point of tissue sections observed by microscopy.
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spelling pubmed-98561922023-01-21 Urinary Proteome Changes during Pregnancy in Rats Tang, Shuxuan Gao, Youhe Biomolecules Article Pregnancy involves a significant number of physiological changes. A normal pregnancy is essential to ensure healthy maternal and fetal development. We sought to explore whether the urinary proteome could reflect the pregnancy process. Urine samples were collected from pregnant and control rats on various gestational days. The urinary proteome was profiled by liquid chromatography coupled with tandem mass spectrometry (LC–MS/MS), and differential proteins were obtained by comparing to the gestational day 1 of the same group at each time point. Many pathways related to embryo implantation and trophoblast differentiation were enriched in the early days in urine. Liver, kidney, and bone development started early to be enriched in the pregnant group, but not in the control group. Interestingly, the developmental processes of the fetal heart such as heart looping and endocardial cushion formation could be seen in urine of pregnant rats. Moreover, the timings were consistent with those of embryological studies. The timing of the surfactant appearance in urine was right before birth. The differential proteins related to pancreas development appeared in urine at the time during reported time of pancreatic cell proliferation and differentiation. These processes were enriched only in the pregnant group and not in the control group. Furthermore, coagulation-associated pathways were found to be increasingly prominent before labor. Our results indicated that the urine proteome of pregnant rats can reflect the process of pregnancy, even fetal embryonic development. Maternal urinary proteome detection was earlier than the developmental time point of tissue sections observed by microscopy. MDPI 2022-12-24 /pmc/articles/PMC9856192/ /pubmed/36671419 http://dx.doi.org/10.3390/biom13010034 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tang, Shuxuan
Gao, Youhe
Urinary Proteome Changes during Pregnancy in Rats
title Urinary Proteome Changes during Pregnancy in Rats
title_full Urinary Proteome Changes during Pregnancy in Rats
title_fullStr Urinary Proteome Changes during Pregnancy in Rats
title_full_unstemmed Urinary Proteome Changes during Pregnancy in Rats
title_short Urinary Proteome Changes during Pregnancy in Rats
title_sort urinary proteome changes during pregnancy in rats
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9856192/
https://www.ncbi.nlm.nih.gov/pubmed/36671419
http://dx.doi.org/10.3390/biom13010034
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