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A Combination of Taurine and Caffeine in Stallion Semen Extender Positively Affects the Spermatozoa Parameters

This study was aimed to determine the impact of different taurine and caffeine combinations on the motility, viability, and oxidative markers of chilled stallion spermatozoa. Each stallion semen sample was diluted in a ratio of 1:2, with various taurine and caffeine concentrations (2.5–7.5 mg/mL tau...

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Autores principales: Halo, Marko, Tirpák, Filip, Slanina, Tomáš, Tokárová, Katarína, Massányi, Martin, Dianová, Lucia, Mlyneková, Eva, Greń, Agnieszka, Massányi, Peter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9856288/
https://www.ncbi.nlm.nih.gov/pubmed/36672253
http://dx.doi.org/10.3390/cells12020320
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author Halo, Marko
Tirpák, Filip
Slanina, Tomáš
Tokárová, Katarína
Massányi, Martin
Dianová, Lucia
Mlyneková, Eva
Greń, Agnieszka
Halo, Marko
Massányi, Peter
author_facet Halo, Marko
Tirpák, Filip
Slanina, Tomáš
Tokárová, Katarína
Massányi, Martin
Dianová, Lucia
Mlyneková, Eva
Greń, Agnieszka
Halo, Marko
Massányi, Peter
author_sort Halo, Marko
collection PubMed
description This study was aimed to determine the impact of different taurine and caffeine combinations on the motility, viability, and oxidative markers of chilled stallion spermatozoa. Each stallion semen sample was diluted in a ratio of 1:2, with various taurine and caffeine concentrations (2.5–7.5 mg/mL taurine + 0.625–1.25 mg/mL caffeine) dissolved in a conventional extender. The control samples (CON) were prepared by diluting ejaculate only using the conventional extender. The motility was analyzed using a CASA system at different time intervals (0, 6, 12, 24, and 30 h) and the viability was evaluated using a mitochondrial toxicity test (MTT) performed at the end of the incubation at 5 °C. The liquid part of experimental samples was separated by centrifugation after 30 h of incubation and underwent the evaluation of oxidative stress via the quantification of markers ferric reducing ability of plasma (FRAP) and total oxidant status (TOS). The samples that were treated with a combination of taurine and caffeine significantly improved the motility parameters, mainly after 12, 24, and 30 h of incubation. Samples extended with combination of taurine and caffeine neither compromise viability nor alterations of redox status. The results of this study describe the combination of taurine and caffeine as an optimal supplement for improving the quality of stallion semen during chilled storage.
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spelling pubmed-98562882023-01-21 A Combination of Taurine and Caffeine in Stallion Semen Extender Positively Affects the Spermatozoa Parameters Halo, Marko Tirpák, Filip Slanina, Tomáš Tokárová, Katarína Massányi, Martin Dianová, Lucia Mlyneková, Eva Greń, Agnieszka Halo, Marko Massányi, Peter Cells Article This study was aimed to determine the impact of different taurine and caffeine combinations on the motility, viability, and oxidative markers of chilled stallion spermatozoa. Each stallion semen sample was diluted in a ratio of 1:2, with various taurine and caffeine concentrations (2.5–7.5 mg/mL taurine + 0.625–1.25 mg/mL caffeine) dissolved in a conventional extender. The control samples (CON) were prepared by diluting ejaculate only using the conventional extender. The motility was analyzed using a CASA system at different time intervals (0, 6, 12, 24, and 30 h) and the viability was evaluated using a mitochondrial toxicity test (MTT) performed at the end of the incubation at 5 °C. The liquid part of experimental samples was separated by centrifugation after 30 h of incubation and underwent the evaluation of oxidative stress via the quantification of markers ferric reducing ability of plasma (FRAP) and total oxidant status (TOS). The samples that were treated with a combination of taurine and caffeine significantly improved the motility parameters, mainly after 12, 24, and 30 h of incubation. Samples extended with combination of taurine and caffeine neither compromise viability nor alterations of redox status. The results of this study describe the combination of taurine and caffeine as an optimal supplement for improving the quality of stallion semen during chilled storage. MDPI 2023-01-14 /pmc/articles/PMC9856288/ /pubmed/36672253 http://dx.doi.org/10.3390/cells12020320 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Halo, Marko
Tirpák, Filip
Slanina, Tomáš
Tokárová, Katarína
Massányi, Martin
Dianová, Lucia
Mlyneková, Eva
Greń, Agnieszka
Halo, Marko
Massányi, Peter
A Combination of Taurine and Caffeine in Stallion Semen Extender Positively Affects the Spermatozoa Parameters
title A Combination of Taurine and Caffeine in Stallion Semen Extender Positively Affects the Spermatozoa Parameters
title_full A Combination of Taurine and Caffeine in Stallion Semen Extender Positively Affects the Spermatozoa Parameters
title_fullStr A Combination of Taurine and Caffeine in Stallion Semen Extender Positively Affects the Spermatozoa Parameters
title_full_unstemmed A Combination of Taurine and Caffeine in Stallion Semen Extender Positively Affects the Spermatozoa Parameters
title_short A Combination of Taurine and Caffeine in Stallion Semen Extender Positively Affects the Spermatozoa Parameters
title_sort combination of taurine and caffeine in stallion semen extender positively affects the spermatozoa parameters
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9856288/
https://www.ncbi.nlm.nih.gov/pubmed/36672253
http://dx.doi.org/10.3390/cells12020320
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