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The Generation of Human iPSC Lines from Three Individuals with Dravet Syndrome and Characterization of Neural Differentiation Markers in iPSC-Derived Ventral Forebrain Organoid Model

Dravet syndrome (DRVT) is a rare form of neurodevelopmental disorder with a high risk of sudden unexpected death in epilepsy (SUDEP), caused mainly (>80% cases) by mutations in the SCN1A gene, coding the Nav1.1 protein (alfa-subunit of voltage-sensitive sodium channel). Mutations in SCN1A are lin...

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Autores principales: Zayat, Valery, Kuczynska, Zuzanna, Liput, Michal, Metin, Erkan, Rzonca-Niewczas, Sylwia, Smyk, Marta, Mazurczak, Tomasz, Goszczanska-Ciuchta, Alicja, Leszczynski, Pawel, Hoffman-Zacharska, Dorota, Buzanska, Leonora
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9856691/
https://www.ncbi.nlm.nih.gov/pubmed/36672274
http://dx.doi.org/10.3390/cells12020339
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author Zayat, Valery
Kuczynska, Zuzanna
Liput, Michal
Metin, Erkan
Rzonca-Niewczas, Sylwia
Smyk, Marta
Mazurczak, Tomasz
Goszczanska-Ciuchta, Alicja
Leszczynski, Pawel
Hoffman-Zacharska, Dorota
Buzanska, Leonora
author_facet Zayat, Valery
Kuczynska, Zuzanna
Liput, Michal
Metin, Erkan
Rzonca-Niewczas, Sylwia
Smyk, Marta
Mazurczak, Tomasz
Goszczanska-Ciuchta, Alicja
Leszczynski, Pawel
Hoffman-Zacharska, Dorota
Buzanska, Leonora
author_sort Zayat, Valery
collection PubMed
description Dravet syndrome (DRVT) is a rare form of neurodevelopmental disorder with a high risk of sudden unexpected death in epilepsy (SUDEP), caused mainly (>80% cases) by mutations in the SCN1A gene, coding the Nav1.1 protein (alfa-subunit of voltage-sensitive sodium channel). Mutations in SCN1A are linked to heterogenous epileptic phenotypes of various types, severity, and patient prognosis. Here we generated iPSC lines from fibroblasts obtained from three individuals affected with DRVT carrying distinct mutations in the SCN1A gene (nonsense mutation p.Ser1516*, missense mutation p.Arg1596His, and splicing mutation c.2589+2dupT). The iPSC lines, generated with the non-integrative approach, retained the distinct SCN1A gene mutation of the donor fibroblasts and were characterized by confirming the expression of the pluripotency markers, the three-germ layer differentiation potential, the absence of exogenous vector expression, and a normal karyotype. The generated iPSC lines were used to establish ventral forebrain organoids, the most affected type of neurons in the pathology of DRVT. The DRVT organoid model will provide an additional resource for deciphering the pathology behind Nav1.1 haploinsufficiency and drug screening to remediate the functional deficits associated with the disease.
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spelling pubmed-98566912023-01-21 The Generation of Human iPSC Lines from Three Individuals with Dravet Syndrome and Characterization of Neural Differentiation Markers in iPSC-Derived Ventral Forebrain Organoid Model Zayat, Valery Kuczynska, Zuzanna Liput, Michal Metin, Erkan Rzonca-Niewczas, Sylwia Smyk, Marta Mazurczak, Tomasz Goszczanska-Ciuchta, Alicja Leszczynski, Pawel Hoffman-Zacharska, Dorota Buzanska, Leonora Cells Article Dravet syndrome (DRVT) is a rare form of neurodevelopmental disorder with a high risk of sudden unexpected death in epilepsy (SUDEP), caused mainly (>80% cases) by mutations in the SCN1A gene, coding the Nav1.1 protein (alfa-subunit of voltage-sensitive sodium channel). Mutations in SCN1A are linked to heterogenous epileptic phenotypes of various types, severity, and patient prognosis. Here we generated iPSC lines from fibroblasts obtained from three individuals affected with DRVT carrying distinct mutations in the SCN1A gene (nonsense mutation p.Ser1516*, missense mutation p.Arg1596His, and splicing mutation c.2589+2dupT). The iPSC lines, generated with the non-integrative approach, retained the distinct SCN1A gene mutation of the donor fibroblasts and were characterized by confirming the expression of the pluripotency markers, the three-germ layer differentiation potential, the absence of exogenous vector expression, and a normal karyotype. The generated iPSC lines were used to establish ventral forebrain organoids, the most affected type of neurons in the pathology of DRVT. The DRVT organoid model will provide an additional resource for deciphering the pathology behind Nav1.1 haploinsufficiency and drug screening to remediate the functional deficits associated with the disease. MDPI 2023-01-16 /pmc/articles/PMC9856691/ /pubmed/36672274 http://dx.doi.org/10.3390/cells12020339 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zayat, Valery
Kuczynska, Zuzanna
Liput, Michal
Metin, Erkan
Rzonca-Niewczas, Sylwia
Smyk, Marta
Mazurczak, Tomasz
Goszczanska-Ciuchta, Alicja
Leszczynski, Pawel
Hoffman-Zacharska, Dorota
Buzanska, Leonora
The Generation of Human iPSC Lines from Three Individuals with Dravet Syndrome and Characterization of Neural Differentiation Markers in iPSC-Derived Ventral Forebrain Organoid Model
title The Generation of Human iPSC Lines from Three Individuals with Dravet Syndrome and Characterization of Neural Differentiation Markers in iPSC-Derived Ventral Forebrain Organoid Model
title_full The Generation of Human iPSC Lines from Three Individuals with Dravet Syndrome and Characterization of Neural Differentiation Markers in iPSC-Derived Ventral Forebrain Organoid Model
title_fullStr The Generation of Human iPSC Lines from Three Individuals with Dravet Syndrome and Characterization of Neural Differentiation Markers in iPSC-Derived Ventral Forebrain Organoid Model
title_full_unstemmed The Generation of Human iPSC Lines from Three Individuals with Dravet Syndrome and Characterization of Neural Differentiation Markers in iPSC-Derived Ventral Forebrain Organoid Model
title_short The Generation of Human iPSC Lines from Three Individuals with Dravet Syndrome and Characterization of Neural Differentiation Markers in iPSC-Derived Ventral Forebrain Organoid Model
title_sort generation of human ipsc lines from three individuals with dravet syndrome and characterization of neural differentiation markers in ipsc-derived ventral forebrain organoid model
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9856691/
https://www.ncbi.nlm.nih.gov/pubmed/36672274
http://dx.doi.org/10.3390/cells12020339
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