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The Antineoplastic Effect of Dimethyl Fumarate on Virus-Negative Merkel Cell Carcinoma Cell Lines: Preliminary Results
SIMPLE SUMMARY: Merkel cell carcinoma (MCC) is an aggressive skin cancer with limited treatment options for the advanced disease once immunotherapy has failed. MCC carcinogenesis has been attributed to the integration of an oncogenic virus or chronic UV radiation. The antineoplastic effects of dimet...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9857057/ https://www.ncbi.nlm.nih.gov/pubmed/36672496 http://dx.doi.org/10.3390/cancers15020547 |
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author | Gambichler, Thilo Brüggestrat, Lyn G. Skrygan, Marina Scheel, Christina H. Susok, Laura Becker, Jürgen C. |
author_facet | Gambichler, Thilo Brüggestrat, Lyn G. Skrygan, Marina Scheel, Christina H. Susok, Laura Becker, Jürgen C. |
author_sort | Gambichler, Thilo |
collection | PubMed |
description | SIMPLE SUMMARY: Merkel cell carcinoma (MCC) is an aggressive skin cancer with limited treatment options for the advanced disease once immunotherapy has failed. MCC carcinogenesis has been attributed to the integration of an oncogenic virus or chronic UV radiation. The antineoplastic effects of dimethyl fumarate (DMF) have recently been shown in melanoma. Three virus-negative UV-associated MCC cell lines were treated with different doses of DMF. The cytotoxic effects and cell proliferation were assessed by the MTT cytotoxicity assay and BrdU proliferation assay for four different treatment periods. The reductions in cell viability and proliferation were demonstrated for all the cell lines used, with DMF proving to be significantly effective. ABSTRACT: Merkel cell carcinoma (MCC) is a rare, difficult-to-treat skin cancer once immunotherapy has failed. MCC is associated either with the clonal integration of the Merkel cell polyomavirus (MCPyV) or mutagenic UV-radiation. Fumaric acid esters, including dimethyl fumarate (DMF), have been shown to inhibit cell growth in cutaneous melanoma and lymphoma. We aimed to explore the effects of DMF on MCPyV-negative MCC cell lines. Three MCC cell lines (MCC13, MCC14.2, and MCC26) were treated with different doses of DMF. The cytotoxic effects and cell proliferation were assessed by the MTT cytotoxicity assay and BrdU proliferation assay at different time points. A significant reduction in cell viability and proliferation were demonstrated for all the cell lines used, with DMF proving to be effective. |
format | Online Article Text |
id | pubmed-9857057 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-98570572023-01-21 The Antineoplastic Effect of Dimethyl Fumarate on Virus-Negative Merkel Cell Carcinoma Cell Lines: Preliminary Results Gambichler, Thilo Brüggestrat, Lyn G. Skrygan, Marina Scheel, Christina H. Susok, Laura Becker, Jürgen C. Cancers (Basel) Article SIMPLE SUMMARY: Merkel cell carcinoma (MCC) is an aggressive skin cancer with limited treatment options for the advanced disease once immunotherapy has failed. MCC carcinogenesis has been attributed to the integration of an oncogenic virus or chronic UV radiation. The antineoplastic effects of dimethyl fumarate (DMF) have recently been shown in melanoma. Three virus-negative UV-associated MCC cell lines were treated with different doses of DMF. The cytotoxic effects and cell proliferation were assessed by the MTT cytotoxicity assay and BrdU proliferation assay for four different treatment periods. The reductions in cell viability and proliferation were demonstrated for all the cell lines used, with DMF proving to be significantly effective. ABSTRACT: Merkel cell carcinoma (MCC) is a rare, difficult-to-treat skin cancer once immunotherapy has failed. MCC is associated either with the clonal integration of the Merkel cell polyomavirus (MCPyV) or mutagenic UV-radiation. Fumaric acid esters, including dimethyl fumarate (DMF), have been shown to inhibit cell growth in cutaneous melanoma and lymphoma. We aimed to explore the effects of DMF on MCPyV-negative MCC cell lines. Three MCC cell lines (MCC13, MCC14.2, and MCC26) were treated with different doses of DMF. The cytotoxic effects and cell proliferation were assessed by the MTT cytotoxicity assay and BrdU proliferation assay at different time points. A significant reduction in cell viability and proliferation were demonstrated for all the cell lines used, with DMF proving to be effective. MDPI 2023-01-16 /pmc/articles/PMC9857057/ /pubmed/36672496 http://dx.doi.org/10.3390/cancers15020547 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Gambichler, Thilo Brüggestrat, Lyn G. Skrygan, Marina Scheel, Christina H. Susok, Laura Becker, Jürgen C. The Antineoplastic Effect of Dimethyl Fumarate on Virus-Negative Merkel Cell Carcinoma Cell Lines: Preliminary Results |
title | The Antineoplastic Effect of Dimethyl Fumarate on Virus-Negative Merkel Cell Carcinoma Cell Lines: Preliminary Results |
title_full | The Antineoplastic Effect of Dimethyl Fumarate on Virus-Negative Merkel Cell Carcinoma Cell Lines: Preliminary Results |
title_fullStr | The Antineoplastic Effect of Dimethyl Fumarate on Virus-Negative Merkel Cell Carcinoma Cell Lines: Preliminary Results |
title_full_unstemmed | The Antineoplastic Effect of Dimethyl Fumarate on Virus-Negative Merkel Cell Carcinoma Cell Lines: Preliminary Results |
title_short | The Antineoplastic Effect of Dimethyl Fumarate on Virus-Negative Merkel Cell Carcinoma Cell Lines: Preliminary Results |
title_sort | antineoplastic effect of dimethyl fumarate on virus-negative merkel cell carcinoma cell lines: preliminary results |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9857057/ https://www.ncbi.nlm.nih.gov/pubmed/36672496 http://dx.doi.org/10.3390/cancers15020547 |
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