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The Antineoplastic Effect of Dimethyl Fumarate on Virus-Negative Merkel Cell Carcinoma Cell Lines: Preliminary Results

SIMPLE SUMMARY: Merkel cell carcinoma (MCC) is an aggressive skin cancer with limited treatment options for the advanced disease once immunotherapy has failed. MCC carcinogenesis has been attributed to the integration of an oncogenic virus or chronic UV radiation. The antineoplastic effects of dimet...

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Autores principales: Gambichler, Thilo, Brüggestrat, Lyn G., Skrygan, Marina, Scheel, Christina H., Susok, Laura, Becker, Jürgen C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9857057/
https://www.ncbi.nlm.nih.gov/pubmed/36672496
http://dx.doi.org/10.3390/cancers15020547
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author Gambichler, Thilo
Brüggestrat, Lyn G.
Skrygan, Marina
Scheel, Christina H.
Susok, Laura
Becker, Jürgen C.
author_facet Gambichler, Thilo
Brüggestrat, Lyn G.
Skrygan, Marina
Scheel, Christina H.
Susok, Laura
Becker, Jürgen C.
author_sort Gambichler, Thilo
collection PubMed
description SIMPLE SUMMARY: Merkel cell carcinoma (MCC) is an aggressive skin cancer with limited treatment options for the advanced disease once immunotherapy has failed. MCC carcinogenesis has been attributed to the integration of an oncogenic virus or chronic UV radiation. The antineoplastic effects of dimethyl fumarate (DMF) have recently been shown in melanoma. Three virus-negative UV-associated MCC cell lines were treated with different doses of DMF. The cytotoxic effects and cell proliferation were assessed by the MTT cytotoxicity assay and BrdU proliferation assay for four different treatment periods. The reductions in cell viability and proliferation were demonstrated for all the cell lines used, with DMF proving to be significantly effective. ABSTRACT: Merkel cell carcinoma (MCC) is a rare, difficult-to-treat skin cancer once immunotherapy has failed. MCC is associated either with the clonal integration of the Merkel cell polyomavirus (MCPyV) or mutagenic UV-radiation. Fumaric acid esters, including dimethyl fumarate (DMF), have been shown to inhibit cell growth in cutaneous melanoma and lymphoma. We aimed to explore the effects of DMF on MCPyV-negative MCC cell lines. Three MCC cell lines (MCC13, MCC14.2, and MCC26) were treated with different doses of DMF. The cytotoxic effects and cell proliferation were assessed by the MTT cytotoxicity assay and BrdU proliferation assay at different time points. A significant reduction in cell viability and proliferation were demonstrated for all the cell lines used, with DMF proving to be effective.
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spelling pubmed-98570572023-01-21 The Antineoplastic Effect of Dimethyl Fumarate on Virus-Negative Merkel Cell Carcinoma Cell Lines: Preliminary Results Gambichler, Thilo Brüggestrat, Lyn G. Skrygan, Marina Scheel, Christina H. Susok, Laura Becker, Jürgen C. Cancers (Basel) Article SIMPLE SUMMARY: Merkel cell carcinoma (MCC) is an aggressive skin cancer with limited treatment options for the advanced disease once immunotherapy has failed. MCC carcinogenesis has been attributed to the integration of an oncogenic virus or chronic UV radiation. The antineoplastic effects of dimethyl fumarate (DMF) have recently been shown in melanoma. Three virus-negative UV-associated MCC cell lines were treated with different doses of DMF. The cytotoxic effects and cell proliferation were assessed by the MTT cytotoxicity assay and BrdU proliferation assay for four different treatment periods. The reductions in cell viability and proliferation were demonstrated for all the cell lines used, with DMF proving to be significantly effective. ABSTRACT: Merkel cell carcinoma (MCC) is a rare, difficult-to-treat skin cancer once immunotherapy has failed. MCC is associated either with the clonal integration of the Merkel cell polyomavirus (MCPyV) or mutagenic UV-radiation. Fumaric acid esters, including dimethyl fumarate (DMF), have been shown to inhibit cell growth in cutaneous melanoma and lymphoma. We aimed to explore the effects of DMF on MCPyV-negative MCC cell lines. Three MCC cell lines (MCC13, MCC14.2, and MCC26) were treated with different doses of DMF. The cytotoxic effects and cell proliferation were assessed by the MTT cytotoxicity assay and BrdU proliferation assay at different time points. A significant reduction in cell viability and proliferation were demonstrated for all the cell lines used, with DMF proving to be effective. MDPI 2023-01-16 /pmc/articles/PMC9857057/ /pubmed/36672496 http://dx.doi.org/10.3390/cancers15020547 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Gambichler, Thilo
Brüggestrat, Lyn G.
Skrygan, Marina
Scheel, Christina H.
Susok, Laura
Becker, Jürgen C.
The Antineoplastic Effect of Dimethyl Fumarate on Virus-Negative Merkel Cell Carcinoma Cell Lines: Preliminary Results
title The Antineoplastic Effect of Dimethyl Fumarate on Virus-Negative Merkel Cell Carcinoma Cell Lines: Preliminary Results
title_full The Antineoplastic Effect of Dimethyl Fumarate on Virus-Negative Merkel Cell Carcinoma Cell Lines: Preliminary Results
title_fullStr The Antineoplastic Effect of Dimethyl Fumarate on Virus-Negative Merkel Cell Carcinoma Cell Lines: Preliminary Results
title_full_unstemmed The Antineoplastic Effect of Dimethyl Fumarate on Virus-Negative Merkel Cell Carcinoma Cell Lines: Preliminary Results
title_short The Antineoplastic Effect of Dimethyl Fumarate on Virus-Negative Merkel Cell Carcinoma Cell Lines: Preliminary Results
title_sort antineoplastic effect of dimethyl fumarate on virus-negative merkel cell carcinoma cell lines: preliminary results
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9857057/
https://www.ncbi.nlm.nih.gov/pubmed/36672496
http://dx.doi.org/10.3390/cancers15020547
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