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In Vitro Antiviral Evaluations of Coldmix(®): An Essential Oil Blend against SARS-CoV-2

Coldmix(®) is a commercially available Eucalyptus aetheroleum and, Abies aetheroleum blend for medicinal applications. In this present study, the in vitro antiviral potential of Coldmix(®), and its major constituents 1,8-cineole and α-pinene were evaluated by using the in vitro ACE2 enzyme inhibitio...

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Autores principales: Başer, Kemal Hüsnü Can, Karadağ, Ayşe Esra, Biltekin, Sevde Nur, Ertürk, Murat, Demirci, Fatih
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9857070/
https://www.ncbi.nlm.nih.gov/pubmed/36661531
http://dx.doi.org/10.3390/cimb45010045
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author Başer, Kemal Hüsnü Can
Karadağ, Ayşe Esra
Biltekin, Sevde Nur
Ertürk, Murat
Demirci, Fatih
author_facet Başer, Kemal Hüsnü Can
Karadağ, Ayşe Esra
Biltekin, Sevde Nur
Ertürk, Murat
Demirci, Fatih
author_sort Başer, Kemal Hüsnü Can
collection PubMed
description Coldmix(®) is a commercially available Eucalyptus aetheroleum and, Abies aetheroleum blend for medicinal applications. In this present study, the in vitro antiviral potential of Coldmix(®), and its major constituents 1,8-cineole and α-pinene were evaluated by using the in vitro ACE2 enzyme inhibition assay as well as the direct contact test against SARS-CoV-2. The observed ACE2 enzyme inhibitory activity of Coldmix(®), 1,8-cineole, and α-pinene were 72%, 88%, and 80%, respectively; whereas in the direct contact test in the vapor phase, the destruction of the virus was 79.9% within 5 min and 93.2% in the 30th min, respectively. In a similar Coldmix(®) vapor phase setup using the in vitro cytotoxicity cell assay, E6 VERO healthy cells were experimentally not affected by toxicity. According to the promising initial antiviral results of Coldmix(®) and the individually tested constituents, detailed further in vivo evaluation using different virus classes is suggested.
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spelling pubmed-98570702023-01-21 In Vitro Antiviral Evaluations of Coldmix(®): An Essential Oil Blend against SARS-CoV-2 Başer, Kemal Hüsnü Can Karadağ, Ayşe Esra Biltekin, Sevde Nur Ertürk, Murat Demirci, Fatih Curr Issues Mol Biol Article Coldmix(®) is a commercially available Eucalyptus aetheroleum and, Abies aetheroleum blend for medicinal applications. In this present study, the in vitro antiviral potential of Coldmix(®), and its major constituents 1,8-cineole and α-pinene were evaluated by using the in vitro ACE2 enzyme inhibition assay as well as the direct contact test against SARS-CoV-2. The observed ACE2 enzyme inhibitory activity of Coldmix(®), 1,8-cineole, and α-pinene were 72%, 88%, and 80%, respectively; whereas in the direct contact test in the vapor phase, the destruction of the virus was 79.9% within 5 min and 93.2% in the 30th min, respectively. In a similar Coldmix(®) vapor phase setup using the in vitro cytotoxicity cell assay, E6 VERO healthy cells were experimentally not affected by toxicity. According to the promising initial antiviral results of Coldmix(®) and the individually tested constituents, detailed further in vivo evaluation using different virus classes is suggested. MDPI 2023-01-11 /pmc/articles/PMC9857070/ /pubmed/36661531 http://dx.doi.org/10.3390/cimb45010045 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Başer, Kemal Hüsnü Can
Karadağ, Ayşe Esra
Biltekin, Sevde Nur
Ertürk, Murat
Demirci, Fatih
In Vitro Antiviral Evaluations of Coldmix(®): An Essential Oil Blend against SARS-CoV-2
title In Vitro Antiviral Evaluations of Coldmix(®): An Essential Oil Blend against SARS-CoV-2
title_full In Vitro Antiviral Evaluations of Coldmix(®): An Essential Oil Blend against SARS-CoV-2
title_fullStr In Vitro Antiviral Evaluations of Coldmix(®): An Essential Oil Blend against SARS-CoV-2
title_full_unstemmed In Vitro Antiviral Evaluations of Coldmix(®): An Essential Oil Blend against SARS-CoV-2
title_short In Vitro Antiviral Evaluations of Coldmix(®): An Essential Oil Blend against SARS-CoV-2
title_sort in vitro antiviral evaluations of coldmix(®): an essential oil blend against sars-cov-2
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9857070/
https://www.ncbi.nlm.nih.gov/pubmed/36661531
http://dx.doi.org/10.3390/cimb45010045
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