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The Expression of Testin, Ki-67 and p16 in Cervical Cancer Diagnostics
Testin is a protein expressed in normal human tissues, being responsible, with other cytoskeleton proteins, for the proper functioning of cell–cell junction areas and focal adhesion plaques. It takes part in the regulation of actin filament changes during cell spreading and motility. Loss of heteroz...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9857082/ https://www.ncbi.nlm.nih.gov/pubmed/36661518 http://dx.doi.org/10.3390/cimb45010032 |
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author | Popiel-Kopaczyk, Aneta Grzegrzolka, Jedrzej Piotrowska, Aleksandra Olbromski, Mateusz Smolarz, Beata Romanowicz, Hanna Rusak, Agnieszka Mrozowska, Monika Dziegiel, Piotr Podhorska-Okolow, Marzenna Kobierzycki, Christopher |
author_facet | Popiel-Kopaczyk, Aneta Grzegrzolka, Jedrzej Piotrowska, Aleksandra Olbromski, Mateusz Smolarz, Beata Romanowicz, Hanna Rusak, Agnieszka Mrozowska, Monika Dziegiel, Piotr Podhorska-Okolow, Marzenna Kobierzycki, Christopher |
author_sort | Popiel-Kopaczyk, Aneta |
collection | PubMed |
description | Testin is a protein expressed in normal human tissues, being responsible, with other cytoskeleton proteins, for the proper functioning of cell–cell junction areas and focal adhesion plaques. It takes part in the regulation of actin filament changes during cell spreading and motility. Loss of heterozygosity in the testin-encoding gene results in altered protein expression in many malignancies, as partly described for cervical cancer. The aim of our study was the assessment of the immunohistochemical (IHC) expression of testin in cervical cancer and its analysis in regard to clinical data as well the expression of the Ki-67 antigen and p16 protein. Moreover, testin expression was assessed by Western blot (WB) in commercially available cell lines. The IHC analysis disclosed that the expression of testin inversely correlated with p16 (r = −0.2104, p < 0.0465) and Ki-67 expression (r = −0.2359, p < 0.0278). Moreover, weaker testin expression was observed in cancer cases vs. control ones (p < 0.0113). The WB analysis of testin expression in the cervical cancer cell lines corresponded to the IHC results and showed a weaker expression compared to that in the control cell line. When we compared the expression of testin in cervical cancer cell lines, we found a weaker expression in HPV-negative cell lines. In summary, we found that the intensity of testin expression and the number of positive cells inversely correlated with the expression of Ki-67 (a marker of proliferation) and p16 (a marker of cell cycle dysregulation). This study shows that the combined assessment of testin, Ki-67 and p16 expression may improve cervical cancer diagnostics. |
format | Online Article Text |
id | pubmed-9857082 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-98570822023-01-21 The Expression of Testin, Ki-67 and p16 in Cervical Cancer Diagnostics Popiel-Kopaczyk, Aneta Grzegrzolka, Jedrzej Piotrowska, Aleksandra Olbromski, Mateusz Smolarz, Beata Romanowicz, Hanna Rusak, Agnieszka Mrozowska, Monika Dziegiel, Piotr Podhorska-Okolow, Marzenna Kobierzycki, Christopher Curr Issues Mol Biol Article Testin is a protein expressed in normal human tissues, being responsible, with other cytoskeleton proteins, for the proper functioning of cell–cell junction areas and focal adhesion plaques. It takes part in the regulation of actin filament changes during cell spreading and motility. Loss of heterozygosity in the testin-encoding gene results in altered protein expression in many malignancies, as partly described for cervical cancer. The aim of our study was the assessment of the immunohistochemical (IHC) expression of testin in cervical cancer and its analysis in regard to clinical data as well the expression of the Ki-67 antigen and p16 protein. Moreover, testin expression was assessed by Western blot (WB) in commercially available cell lines. The IHC analysis disclosed that the expression of testin inversely correlated with p16 (r = −0.2104, p < 0.0465) and Ki-67 expression (r = −0.2359, p < 0.0278). Moreover, weaker testin expression was observed in cancer cases vs. control ones (p < 0.0113). The WB analysis of testin expression in the cervical cancer cell lines corresponded to the IHC results and showed a weaker expression compared to that in the control cell line. When we compared the expression of testin in cervical cancer cell lines, we found a weaker expression in HPV-negative cell lines. In summary, we found that the intensity of testin expression and the number of positive cells inversely correlated with the expression of Ki-67 (a marker of proliferation) and p16 (a marker of cell cycle dysregulation). This study shows that the combined assessment of testin, Ki-67 and p16 expression may improve cervical cancer diagnostics. MDPI 2023-01-05 /pmc/articles/PMC9857082/ /pubmed/36661518 http://dx.doi.org/10.3390/cimb45010032 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Popiel-Kopaczyk, Aneta Grzegrzolka, Jedrzej Piotrowska, Aleksandra Olbromski, Mateusz Smolarz, Beata Romanowicz, Hanna Rusak, Agnieszka Mrozowska, Monika Dziegiel, Piotr Podhorska-Okolow, Marzenna Kobierzycki, Christopher The Expression of Testin, Ki-67 and p16 in Cervical Cancer Diagnostics |
title | The Expression of Testin, Ki-67 and p16 in Cervical Cancer Diagnostics |
title_full | The Expression of Testin, Ki-67 and p16 in Cervical Cancer Diagnostics |
title_fullStr | The Expression of Testin, Ki-67 and p16 in Cervical Cancer Diagnostics |
title_full_unstemmed | The Expression of Testin, Ki-67 and p16 in Cervical Cancer Diagnostics |
title_short | The Expression of Testin, Ki-67 and p16 in Cervical Cancer Diagnostics |
title_sort | expression of testin, ki-67 and p16 in cervical cancer diagnostics |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9857082/ https://www.ncbi.nlm.nih.gov/pubmed/36661518 http://dx.doi.org/10.3390/cimb45010032 |
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