Cargando…
SARS-CoV-2 Detection via RT-PCR in Matched Saliva and Nasopharyngeal Samples Reveals High Concordance in Different Commercial Assays
Background: Self-collected saliva samples can increase the diagnostic efficiency and benefit healthcare workers, patient care, and infection control. This study evaluated the performance of self-collected saliva samples compared to nasopharyngeal swabs using three commercial kits for the qualitative...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9857954/ https://www.ncbi.nlm.nih.gov/pubmed/36673140 http://dx.doi.org/10.3390/diagnostics13020329 |
_version_ | 1784873977503547392 |
---|---|
author | de Sousa, Karoline Almeida Felix Nonaka, Carolina Kymie Vasques de Ávila Mendonça, Renata Naves Mascarenhas, Verena Neiva Weber, Thamires Gomes Lopes Regis Silva, Carlos Gustavo Mendes, Ana Verena Almeida Khouri, Ricardo Souza, Bruno Solano Freitas Gurgel Rocha, Clarissa Araújo |
author_facet | de Sousa, Karoline Almeida Felix Nonaka, Carolina Kymie Vasques de Ávila Mendonça, Renata Naves Mascarenhas, Verena Neiva Weber, Thamires Gomes Lopes Regis Silva, Carlos Gustavo Mendes, Ana Verena Almeida Khouri, Ricardo Souza, Bruno Solano Freitas Gurgel Rocha, Clarissa Araújo |
author_sort | de Sousa, Karoline Almeida Felix |
collection | PubMed |
description | Background: Self-collected saliva samples can increase the diagnostic efficiency and benefit healthcare workers, patient care, and infection control. This study evaluated the performance of self-collected saliva samples compared to nasopharyngeal swabs using three commercial kits for the qualitative detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Methods: Matched nasopharyngeal and saliva samples were collected from 103 patients with either asymptomatic or symptomatic COVID-19. Both samples were evaluated using three commercial kits (TaqCheck, Allplex, and TaqPath). To evaluate sample stability, viral RNA extraction was performed in the presence or absence of an RNA-stabilizing solution. Storage conditions, including the duration, temperature, and stability after freezing and thawing of the samples, were also evaluated. Results: All the saliva samples showed 100% concordance with the nasopharyngeal swab results using TaqCheck and Allplex kits, and 93% using TaqPath kit. No difference was observed in the samples that used the RNA-stabilizing solution compared to the group without the solution. The Ct values of the freeze–thawed samples after 30 days were higher than those on day 0; however, the results were consistent the fresh samples. Conclusion: The high concordance of SARS-CoV-2 detection via reverse transcription–polymerase chain reaction (RT-PCR) in matched saliva and nasopharyngeal samples using different commercial assays reinforces the concept that self-collected saliva samples are non-invasive, rapid, and reliable for diagnosing SARS-CoV-2 infection. |
format | Online Article Text |
id | pubmed-9857954 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-98579542023-01-21 SARS-CoV-2 Detection via RT-PCR in Matched Saliva and Nasopharyngeal Samples Reveals High Concordance in Different Commercial Assays de Sousa, Karoline Almeida Felix Nonaka, Carolina Kymie Vasques de Ávila Mendonça, Renata Naves Mascarenhas, Verena Neiva Weber, Thamires Gomes Lopes Regis Silva, Carlos Gustavo Mendes, Ana Verena Almeida Khouri, Ricardo Souza, Bruno Solano Freitas Gurgel Rocha, Clarissa Araújo Diagnostics (Basel) Communication Background: Self-collected saliva samples can increase the diagnostic efficiency and benefit healthcare workers, patient care, and infection control. This study evaluated the performance of self-collected saliva samples compared to nasopharyngeal swabs using three commercial kits for the qualitative detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Methods: Matched nasopharyngeal and saliva samples were collected from 103 patients with either asymptomatic or symptomatic COVID-19. Both samples were evaluated using three commercial kits (TaqCheck, Allplex, and TaqPath). To evaluate sample stability, viral RNA extraction was performed in the presence or absence of an RNA-stabilizing solution. Storage conditions, including the duration, temperature, and stability after freezing and thawing of the samples, were also evaluated. Results: All the saliva samples showed 100% concordance with the nasopharyngeal swab results using TaqCheck and Allplex kits, and 93% using TaqPath kit. No difference was observed in the samples that used the RNA-stabilizing solution compared to the group without the solution. The Ct values of the freeze–thawed samples after 30 days were higher than those on day 0; however, the results were consistent the fresh samples. Conclusion: The high concordance of SARS-CoV-2 detection via reverse transcription–polymerase chain reaction (RT-PCR) in matched saliva and nasopharyngeal samples using different commercial assays reinforces the concept that self-collected saliva samples are non-invasive, rapid, and reliable for diagnosing SARS-CoV-2 infection. MDPI 2023-01-16 /pmc/articles/PMC9857954/ /pubmed/36673140 http://dx.doi.org/10.3390/diagnostics13020329 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Communication de Sousa, Karoline Almeida Felix Nonaka, Carolina Kymie Vasques de Ávila Mendonça, Renata Naves Mascarenhas, Verena Neiva Weber, Thamires Gomes Lopes Regis Silva, Carlos Gustavo Mendes, Ana Verena Almeida Khouri, Ricardo Souza, Bruno Solano Freitas Gurgel Rocha, Clarissa Araújo SARS-CoV-2 Detection via RT-PCR in Matched Saliva and Nasopharyngeal Samples Reveals High Concordance in Different Commercial Assays |
title | SARS-CoV-2 Detection via RT-PCR in Matched Saliva and Nasopharyngeal Samples Reveals High Concordance in Different Commercial Assays |
title_full | SARS-CoV-2 Detection via RT-PCR in Matched Saliva and Nasopharyngeal Samples Reveals High Concordance in Different Commercial Assays |
title_fullStr | SARS-CoV-2 Detection via RT-PCR in Matched Saliva and Nasopharyngeal Samples Reveals High Concordance in Different Commercial Assays |
title_full_unstemmed | SARS-CoV-2 Detection via RT-PCR in Matched Saliva and Nasopharyngeal Samples Reveals High Concordance in Different Commercial Assays |
title_short | SARS-CoV-2 Detection via RT-PCR in Matched Saliva and Nasopharyngeal Samples Reveals High Concordance in Different Commercial Assays |
title_sort | sars-cov-2 detection via rt-pcr in matched saliva and nasopharyngeal samples reveals high concordance in different commercial assays |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9857954/ https://www.ncbi.nlm.nih.gov/pubmed/36673140 http://dx.doi.org/10.3390/diagnostics13020329 |
work_keys_str_mv | AT desousakarolinealmeidafelix sarscov2detectionviartpcrinmatchedsalivaandnasopharyngealsamplesrevealshighconcordanceindifferentcommercialassays AT nonakacarolinakymievasques sarscov2detectionviartpcrinmatchedsalivaandnasopharyngealsamplesrevealshighconcordanceindifferentcommercialassays AT deavilamendoncarenatanaves sarscov2detectionviartpcrinmatchedsalivaandnasopharyngealsamplesrevealshighconcordanceindifferentcommercialassays AT mascarenhasverenaneiva sarscov2detectionviartpcrinmatchedsalivaandnasopharyngealsamplesrevealshighconcordanceindifferentcommercialassays AT weberthamiresgomeslopes sarscov2detectionviartpcrinmatchedsalivaandnasopharyngealsamplesrevealshighconcordanceindifferentcommercialassays AT regissilvacarlosgustavo sarscov2detectionviartpcrinmatchedsalivaandnasopharyngealsamplesrevealshighconcordanceindifferentcommercialassays AT mendesanaverenaalmeida sarscov2detectionviartpcrinmatchedsalivaandnasopharyngealsamplesrevealshighconcordanceindifferentcommercialassays AT khouriricardo sarscov2detectionviartpcrinmatchedsalivaandnasopharyngealsamplesrevealshighconcordanceindifferentcommercialassays AT souzabrunosolanofreitas sarscov2detectionviartpcrinmatchedsalivaandnasopharyngealsamplesrevealshighconcordanceindifferentcommercialassays AT gurgelrochaclarissaaraujo sarscov2detectionviartpcrinmatchedsalivaandnasopharyngealsamplesrevealshighconcordanceindifferentcommercialassays |