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SARS-CoV-2 Detection via RT-PCR in Matched Saliva and Nasopharyngeal Samples Reveals High Concordance in Different Commercial Assays

Background: Self-collected saliva samples can increase the diagnostic efficiency and benefit healthcare workers, patient care, and infection control. This study evaluated the performance of self-collected saliva samples compared to nasopharyngeal swabs using three commercial kits for the qualitative...

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Autores principales: de Sousa, Karoline Almeida Felix, Nonaka, Carolina Kymie Vasques, de Ávila Mendonça, Renata Naves, Mascarenhas, Verena Neiva, Weber, Thamires Gomes Lopes, Regis Silva, Carlos Gustavo, Mendes, Ana Verena Almeida, Khouri, Ricardo, Souza, Bruno Solano Freitas, Gurgel Rocha, Clarissa Araújo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9857954/
https://www.ncbi.nlm.nih.gov/pubmed/36673140
http://dx.doi.org/10.3390/diagnostics13020329
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author de Sousa, Karoline Almeida Felix
Nonaka, Carolina Kymie Vasques
de Ávila Mendonça, Renata Naves
Mascarenhas, Verena Neiva
Weber, Thamires Gomes Lopes
Regis Silva, Carlos Gustavo
Mendes, Ana Verena Almeida
Khouri, Ricardo
Souza, Bruno Solano Freitas
Gurgel Rocha, Clarissa Araújo
author_facet de Sousa, Karoline Almeida Felix
Nonaka, Carolina Kymie Vasques
de Ávila Mendonça, Renata Naves
Mascarenhas, Verena Neiva
Weber, Thamires Gomes Lopes
Regis Silva, Carlos Gustavo
Mendes, Ana Verena Almeida
Khouri, Ricardo
Souza, Bruno Solano Freitas
Gurgel Rocha, Clarissa Araújo
author_sort de Sousa, Karoline Almeida Felix
collection PubMed
description Background: Self-collected saliva samples can increase the diagnostic efficiency and benefit healthcare workers, patient care, and infection control. This study evaluated the performance of self-collected saliva samples compared to nasopharyngeal swabs using three commercial kits for the qualitative detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Methods: Matched nasopharyngeal and saliva samples were collected from 103 patients with either asymptomatic or symptomatic COVID-19. Both samples were evaluated using three commercial kits (TaqCheck, Allplex, and TaqPath). To evaluate sample stability, viral RNA extraction was performed in the presence or absence of an RNA-stabilizing solution. Storage conditions, including the duration, temperature, and stability after freezing and thawing of the samples, were also evaluated. Results: All the saliva samples showed 100% concordance with the nasopharyngeal swab results using TaqCheck and Allplex kits, and 93% using TaqPath kit. No difference was observed in the samples that used the RNA-stabilizing solution compared to the group without the solution. The Ct values of the freeze–thawed samples after 30 days were higher than those on day 0; however, the results were consistent the fresh samples. Conclusion: The high concordance of SARS-CoV-2 detection via reverse transcription–polymerase chain reaction (RT-PCR) in matched saliva and nasopharyngeal samples using different commercial assays reinforces the concept that self-collected saliva samples are non-invasive, rapid, and reliable for diagnosing SARS-CoV-2 infection.
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spelling pubmed-98579542023-01-21 SARS-CoV-2 Detection via RT-PCR in Matched Saliva and Nasopharyngeal Samples Reveals High Concordance in Different Commercial Assays de Sousa, Karoline Almeida Felix Nonaka, Carolina Kymie Vasques de Ávila Mendonça, Renata Naves Mascarenhas, Verena Neiva Weber, Thamires Gomes Lopes Regis Silva, Carlos Gustavo Mendes, Ana Verena Almeida Khouri, Ricardo Souza, Bruno Solano Freitas Gurgel Rocha, Clarissa Araújo Diagnostics (Basel) Communication Background: Self-collected saliva samples can increase the diagnostic efficiency and benefit healthcare workers, patient care, and infection control. This study evaluated the performance of self-collected saliva samples compared to nasopharyngeal swabs using three commercial kits for the qualitative detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Methods: Matched nasopharyngeal and saliva samples were collected from 103 patients with either asymptomatic or symptomatic COVID-19. Both samples were evaluated using three commercial kits (TaqCheck, Allplex, and TaqPath). To evaluate sample stability, viral RNA extraction was performed in the presence or absence of an RNA-stabilizing solution. Storage conditions, including the duration, temperature, and stability after freezing and thawing of the samples, were also evaluated. Results: All the saliva samples showed 100% concordance with the nasopharyngeal swab results using TaqCheck and Allplex kits, and 93% using TaqPath kit. No difference was observed in the samples that used the RNA-stabilizing solution compared to the group without the solution. The Ct values of the freeze–thawed samples after 30 days were higher than those on day 0; however, the results were consistent the fresh samples. Conclusion: The high concordance of SARS-CoV-2 detection via reverse transcription–polymerase chain reaction (RT-PCR) in matched saliva and nasopharyngeal samples using different commercial assays reinforces the concept that self-collected saliva samples are non-invasive, rapid, and reliable for diagnosing SARS-CoV-2 infection. MDPI 2023-01-16 /pmc/articles/PMC9857954/ /pubmed/36673140 http://dx.doi.org/10.3390/diagnostics13020329 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
de Sousa, Karoline Almeida Felix
Nonaka, Carolina Kymie Vasques
de Ávila Mendonça, Renata Naves
Mascarenhas, Verena Neiva
Weber, Thamires Gomes Lopes
Regis Silva, Carlos Gustavo
Mendes, Ana Verena Almeida
Khouri, Ricardo
Souza, Bruno Solano Freitas
Gurgel Rocha, Clarissa Araújo
SARS-CoV-2 Detection via RT-PCR in Matched Saliva and Nasopharyngeal Samples Reveals High Concordance in Different Commercial Assays
title SARS-CoV-2 Detection via RT-PCR in Matched Saliva and Nasopharyngeal Samples Reveals High Concordance in Different Commercial Assays
title_full SARS-CoV-2 Detection via RT-PCR in Matched Saliva and Nasopharyngeal Samples Reveals High Concordance in Different Commercial Assays
title_fullStr SARS-CoV-2 Detection via RT-PCR in Matched Saliva and Nasopharyngeal Samples Reveals High Concordance in Different Commercial Assays
title_full_unstemmed SARS-CoV-2 Detection via RT-PCR in Matched Saliva and Nasopharyngeal Samples Reveals High Concordance in Different Commercial Assays
title_short SARS-CoV-2 Detection via RT-PCR in Matched Saliva and Nasopharyngeal Samples Reveals High Concordance in Different Commercial Assays
title_sort sars-cov-2 detection via rt-pcr in matched saliva and nasopharyngeal samples reveals high concordance in different commercial assays
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9857954/
https://www.ncbi.nlm.nih.gov/pubmed/36673140
http://dx.doi.org/10.3390/diagnostics13020329
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