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Allele-Specific PCR for PIK3CA Mutation Detection Using Phosphoryl Guanidine Modified Primers

Phosphoryl guanidine (PG) is the novel uncharged modification of internucleotide phosphates of oligonucleotides. Incorporating PG modification into PCR primers leads to increased discrimination between wild-type and mutated DNA, providing extraordinary detection limits in an allele-specific real-tim...

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Detalles Bibliográficos
Autores principales:	Chubarov, Alexey S., Oscorbin, Igor P., Novikova, Lidiya M., Filipenko, Maxim L., Lomzov, Alexander A., Pyshnyi, Dmitrii V.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2023
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9858071/ https://www.ncbi.nlm.nih.gov/pubmed/36673060 http://dx.doi.org/10.3390/diagnostics13020250

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