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Detoxification of Fumonisins by Three Novel Transaminases with Diverse Enzymatic Characteristics Coupled with Carboxylesterase
Fumonisin (FB) is one of the most common mycotoxins contaminating feed and food, causing severe public health threat to human and animals worldwide. Until now, only several transaminases were found to reduce FB toxicity, thus, more fumonisin detoxification transaminases with excellent catalytic prop...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9858248/ https://www.ncbi.nlm.nih.gov/pubmed/36673508 http://dx.doi.org/10.3390/foods12020416 |
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author | Wang, Yue Sun, Junhao Zhang, Mengwei Pan, Kungang Liu, Tianhui Zhang, Tongcun Luo, Xuegang Zhao, Junqi Li, Zhongyuan |
author_facet | Wang, Yue Sun, Junhao Zhang, Mengwei Pan, Kungang Liu, Tianhui Zhang, Tongcun Luo, Xuegang Zhao, Junqi Li, Zhongyuan |
author_sort | Wang, Yue |
collection | PubMed |
description | Fumonisin (FB) is one of the most common mycotoxins contaminating feed and food, causing severe public health threat to human and animals worldwide. Until now, only several transaminases were found to reduce FB toxicity, thus, more fumonisin detoxification transaminases with excellent catalytic properties required urgent exploration for complex application conditions. Herein, through gene mining and enzymatic characterization, three novel fumonisin detoxification transaminases—FumTSTA, FumUPTA, FumPHTA—were identified, sharing only 61–74% sequence identity with reported fumonisin detoxification transaminases. Moreover, the recombinant proteins shared diverse pH reaction ranges, good pH stability and thermostability, and the recombinant protein yields were also improved by condition optimum. Furthermore, the final products were analyzed by liquid chromatography-mass spectrometry. This study provides ideal candidates for fumonisin detoxification and meets diverse required demands in food and feed industries. |
format | Online Article Text |
id | pubmed-9858248 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-98582482023-01-21 Detoxification of Fumonisins by Three Novel Transaminases with Diverse Enzymatic Characteristics Coupled with Carboxylesterase Wang, Yue Sun, Junhao Zhang, Mengwei Pan, Kungang Liu, Tianhui Zhang, Tongcun Luo, Xuegang Zhao, Junqi Li, Zhongyuan Foods Article Fumonisin (FB) is one of the most common mycotoxins contaminating feed and food, causing severe public health threat to human and animals worldwide. Until now, only several transaminases were found to reduce FB toxicity, thus, more fumonisin detoxification transaminases with excellent catalytic properties required urgent exploration for complex application conditions. Herein, through gene mining and enzymatic characterization, three novel fumonisin detoxification transaminases—FumTSTA, FumUPTA, FumPHTA—were identified, sharing only 61–74% sequence identity with reported fumonisin detoxification transaminases. Moreover, the recombinant proteins shared diverse pH reaction ranges, good pH stability and thermostability, and the recombinant protein yields were also improved by condition optimum. Furthermore, the final products were analyzed by liquid chromatography-mass spectrometry. This study provides ideal candidates for fumonisin detoxification and meets diverse required demands in food and feed industries. MDPI 2023-01-16 /pmc/articles/PMC9858248/ /pubmed/36673508 http://dx.doi.org/10.3390/foods12020416 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Wang, Yue Sun, Junhao Zhang, Mengwei Pan, Kungang Liu, Tianhui Zhang, Tongcun Luo, Xuegang Zhao, Junqi Li, Zhongyuan Detoxification of Fumonisins by Three Novel Transaminases with Diverse Enzymatic Characteristics Coupled with Carboxylesterase |
title | Detoxification of Fumonisins by Three Novel Transaminases with Diverse Enzymatic Characteristics Coupled with Carboxylesterase |
title_full | Detoxification of Fumonisins by Three Novel Transaminases with Diverse Enzymatic Characteristics Coupled with Carboxylesterase |
title_fullStr | Detoxification of Fumonisins by Three Novel Transaminases with Diverse Enzymatic Characteristics Coupled with Carboxylesterase |
title_full_unstemmed | Detoxification of Fumonisins by Three Novel Transaminases with Diverse Enzymatic Characteristics Coupled with Carboxylesterase |
title_short | Detoxification of Fumonisins by Three Novel Transaminases with Diverse Enzymatic Characteristics Coupled with Carboxylesterase |
title_sort | detoxification of fumonisins by three novel transaminases with diverse enzymatic characteristics coupled with carboxylesterase |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9858248/ https://www.ncbi.nlm.nih.gov/pubmed/36673508 http://dx.doi.org/10.3390/foods12020416 |
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