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Biomechanical properties of porcine meniscus as determined via AFM: Effect of region, compartment and anisotropy

The meniscus is a fibrocartilaginous tissue that plays an essential role in load transmission, lubrication, and stabilization of the knee. Loss of meniscus function, through degeneration or trauma, can lead to osteoarthritis in the underlying articular cartilage. To perform its crucial function, the...

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Autores principales: Orton, Kevin, Batchelor, Wyndham, Ziebarth, Noel M., Best, Thomas M., Travascio, Francesco, Jackson, Alicia R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9858324/
https://www.ncbi.nlm.nih.gov/pubmed/36662701
http://dx.doi.org/10.1371/journal.pone.0280616
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author Orton, Kevin
Batchelor, Wyndham
Ziebarth, Noel M.
Best, Thomas M.
Travascio, Francesco
Jackson, Alicia R.
author_facet Orton, Kevin
Batchelor, Wyndham
Ziebarth, Noel M.
Best, Thomas M.
Travascio, Francesco
Jackson, Alicia R.
author_sort Orton, Kevin
collection PubMed
description The meniscus is a fibrocartilaginous tissue that plays an essential role in load transmission, lubrication, and stabilization of the knee. Loss of meniscus function, through degeneration or trauma, can lead to osteoarthritis in the underlying articular cartilage. To perform its crucial function, the meniscus extracellular matrix has a particular organization, including collagen fiber bundles running circumferentially, allowing the tissue to withstand tensile hoop stresses developed during axial loading. Given its critical role in preserving the health of the knee, better understanding structure-function relations of the biomechanical properties of the meniscus is critical. The main objective of this study was to measure the compressive modulus of porcine meniscus using Atomic Force Microscopy (AFM); the effects of three key factors were investigated: direction (axial, circumferential), compartment (medial, lateral) and region (inner, outer). Porcine menisci were prepared in 8 groups (= 2 directions x 2 compartments x 2 regions) with n = 9 per group. A custom AFM was used to obtain force-indentation curves, which were then curve-fit with the Hertz model to determine the tissue’s compressive modulus. The compressive modulus ranged from 0.75 to 4.00 MPa across the 8 groups, with an averaged value of 2.04±0.86MPa. Only direction had a significant effect on meniscus compressive modulus (circumferential > axial, p = 0.024), in agreement with earlier studies demonstrating that mechanical properties in the tissue are anisotropic. This behavior is likely the result of the particular collagen fiber arrangement in the tissue and plays a key role in load transmission capability. This study provides important information on the micromechanical properties of the meniscus, which is crucial for understanding tissue pathophysiology, as well as for developing novel treatments for tissue repair.
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spelling pubmed-98583242023-01-21 Biomechanical properties of porcine meniscus as determined via AFM: Effect of region, compartment and anisotropy Orton, Kevin Batchelor, Wyndham Ziebarth, Noel M. Best, Thomas M. Travascio, Francesco Jackson, Alicia R. PLoS One Research Article The meniscus is a fibrocartilaginous tissue that plays an essential role in load transmission, lubrication, and stabilization of the knee. Loss of meniscus function, through degeneration or trauma, can lead to osteoarthritis in the underlying articular cartilage. To perform its crucial function, the meniscus extracellular matrix has a particular organization, including collagen fiber bundles running circumferentially, allowing the tissue to withstand tensile hoop stresses developed during axial loading. Given its critical role in preserving the health of the knee, better understanding structure-function relations of the biomechanical properties of the meniscus is critical. The main objective of this study was to measure the compressive modulus of porcine meniscus using Atomic Force Microscopy (AFM); the effects of three key factors were investigated: direction (axial, circumferential), compartment (medial, lateral) and region (inner, outer). Porcine menisci were prepared in 8 groups (= 2 directions x 2 compartments x 2 regions) with n = 9 per group. A custom AFM was used to obtain force-indentation curves, which were then curve-fit with the Hertz model to determine the tissue’s compressive modulus. The compressive modulus ranged from 0.75 to 4.00 MPa across the 8 groups, with an averaged value of 2.04±0.86MPa. Only direction had a significant effect on meniscus compressive modulus (circumferential > axial, p = 0.024), in agreement with earlier studies demonstrating that mechanical properties in the tissue are anisotropic. This behavior is likely the result of the particular collagen fiber arrangement in the tissue and plays a key role in load transmission capability. This study provides important information on the micromechanical properties of the meniscus, which is crucial for understanding tissue pathophysiology, as well as for developing novel treatments for tissue repair. Public Library of Science 2023-01-20 /pmc/articles/PMC9858324/ /pubmed/36662701 http://dx.doi.org/10.1371/journal.pone.0280616 Text en © 2023 Orton et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Orton, Kevin
Batchelor, Wyndham
Ziebarth, Noel M.
Best, Thomas M.
Travascio, Francesco
Jackson, Alicia R.
Biomechanical properties of porcine meniscus as determined via AFM: Effect of region, compartment and anisotropy
title Biomechanical properties of porcine meniscus as determined via AFM: Effect of region, compartment and anisotropy
title_full Biomechanical properties of porcine meniscus as determined via AFM: Effect of region, compartment and anisotropy
title_fullStr Biomechanical properties of porcine meniscus as determined via AFM: Effect of region, compartment and anisotropy
title_full_unstemmed Biomechanical properties of porcine meniscus as determined via AFM: Effect of region, compartment and anisotropy
title_short Biomechanical properties of porcine meniscus as determined via AFM: Effect of region, compartment and anisotropy
title_sort biomechanical properties of porcine meniscus as determined via afm: effect of region, compartment and anisotropy
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9858324/
https://www.ncbi.nlm.nih.gov/pubmed/36662701
http://dx.doi.org/10.1371/journal.pone.0280616
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